3D‐Bioassembly of VH‐Spheroids for Cartilage Regeneration: in Vitro Evaluation of Chondrogenesis, Fusion and Lateral Integration (Adv. Mater. Interfaces 31/2022)

Abstract

Self-assembled vitreous humor (VH) spheroids fulfill key requirements as reproducible building blocks for 3D-bioassembly of cartilage tissues. As described by Gabriella C. J. Lindberg and colleagues in article number 2200882, these VH hydrogels promote both cellular migration and neo-tissue formation in the active interface region of the assembloids, driving successful lateral fusion and integration between mature cartilage spheres while enabling fabrication of larger, clinically relevant, tissue constructs.

A photocrosslinked methacrylated carboxymethyl chitosan/oxidized locust bean gum double network hydrogel for cartilage repair

“…50 GAG is the main ECM glycoprotein of the articular cartilage, and it is a protein that has been modified by large polyanionic carbohydrates (mainly chondroitin sulfate). 51 It binds to hyaluronic acid and draws cations along with osmotically obligated water into the ECM, forming a hydrated gel which gives cartilage its compressive strength. 52 By determining the expression of Col-2 and GAG from chondrocytes co-cultured with the specimens, we are able to further investigate whether the MCOAC hydrogels have better chondrocyte-compatibility.…”