3D-bioprinted double-crosslinked angiogenic alginate/chondroitin sulfate patch for diabetic wound healing

Liao, Weifang; Duan, Xunxin; Xie, Fusheng; Zheng, Dongxi; Pu, Yang; Wang, Xiangguo; Hu, Zhijian

doi:10.1016/j.ijbiomac.2023.123952

Cited by 22 publications

(8 citation statements)

References 37 publications

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“…Wound healing was attempted through fibrosis of the dermal layers, with a shooting fibrotic index of 102.4 ± 5.63 (MT-stained collagen fibers; figure 8(A)). Observations were generally consistent with morphological and morphometric assessments of untreated diabetic wounds [81].…”

Section: Wound Closuresupporting

confidence: 81%

Bioinspired 3D-printed scaffold embedding DDAB-nano ZnO/nanofibrous microspheres for regenerative diabetic wound healing

Metwally,

El-Habashy,

El-Hosseiny

et al. 2023

Biofabrication

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There is a constant demand for novel materials/biomedical devices to accelerate the healing of hard-to-heal wounds. Herein, an innovative 3D-printed bioinspired construct was developed as an antibacterial/regenerative scaffold for diabetic wound healing. Hyaluronic/chitosan (HA/CS) ink was used to fabricate a bilayer scaffold comprising a dense plain hydrogel layer topping an antibacterial/regenerative nanofibrous layer obtained by incorporating the hydrogel with polylactic acid nanofibrous microspheres (MS). These were embedded with nano ZnO (ZNP) or didecyldimethylammonium bromide (DDAB)-treated ZNP (D-ZNP) to generate the antibacterial/healing nano/micro hybrid biomaterials, Z-MS@scaffold and DZ-MS@scaffold. Plain and composite scaffolds incorporating blank MS (blank MS@scaffold) or MS-free ZNP@scaffold and D-ZNP@scaffold were used for comparison. 3D printed bilayer constructs with customizable porosity were obtained as verified by SEM. The DZ-MS@scaffold exhibited the largest total pore area as well as the highest water-uptake capacity and in vitro antibacterial activity. Treatment of Staphylococcus aureus-infected full thickness diabetic wounds in rats indicated superiority of DZ-MS@scaffold as evidenced by multiple assessments. The scaffold afforded 95% wound-closure, infection suppression, effective regulation of healing-associated biomarkers as well as regeneration of skin structure in 14 d. On the other hand, healing of non-diabetic acute wounds was effectively accelerated by the simpler less porous Z-MS@scaffold. Information is provided for the first-time on the 3D printing of nanofibrous scaffolds using non-electrospun injectable bioactive nano/micro particulate constructs, an innovative ZNP-functionalized 3D-printed formulation and the distinct bioactivity of D-ZNP as a powerful antibacterial/wound healing promotor. In addition, findings underscored the crucial role of nanofibrous-MS carrier in enhancing the physicochemical, antibacterial, and wound regenerative properties of DDAB-nano ZnO. In conclusion, innovative 3D-printed DZ-MS@scaffold merging the MS-boosted multiple functionalities of ZNP and DDAB, the structural characteristics of nanofibrous MS in addition to those of the 3D-printed bilayer scaffold, provide a versatile bioactive material platform for diabetic wound healing and other biomedical applications.

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Section: Wound Closuresupporting

confidence: 81%

Bioinspired 3D-printed scaffold embedding DDAB-nano ZnO/nanofibrous microspheres for regenerative diabetic wound healing

Metwally,

El-Habashy,

El-Hosseiny

et al. 2023

Biofabrication

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“…The hydrogel patches were first physical crosslinked using CaCl 2 and then exposed to UV light for photocrosslinking. The VEGF-loaded patch prolonged the growth factor release, exhibiting excellent angiogenic ability and promoting wound healing [ 215 ].…”

Section: Methods Of Preparation For Wound Dressingsmentioning

confidence: 99%

Natural and Synthetic Polymeric Biomaterials for Application in Wound Management

Prete,

Dattilo,

Patitucci

et al. 2023

JFB

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Biomaterials are at the forefront of the future, finding a variety of applications in the biomedical field, especially in wound healing, thanks to their biocompatible and biodegradable properties. Wounds spontaneously try to heal through a series of interconnected processes involving several initiators and mediators such as cytokines, macrophages, and fibroblasts. The combination of biopolymers with wound healing properties may provide opportunities to synthesize matrices that stimulate and trigger target cell responses crucial to the healing process. This review outlines the optimal management and care required for wound treatment with a special focus on biopolymers, drug-delivery systems, and nanotechnologies used for enhanced wound healing applications. Researchers have utilized a range of techniques to produce wound dressings, leading to products with different characteristics. Each method comes with its unique strengths and limitations, which are important to consider. The future trajectory in wound dressing advancement should prioritize economical and eco-friendly methodologies, along with improving the efficacy of constituent materials. The aim of this work is to give researchers the possibility to evaluate the proper materials for wound dressing preparation and to better understand the optimal synthesis conditions as well as the most effective bioactive molecules to load.

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“…Sodium alginate (SA), a cost-effective marine-derived polysaccharide, characterized by excellent biocompatibility, enhanced aqueous solubility and minimal toxicity, has been widely utilized in 3D printing of wound dressings since it can rapidly crosslink with divalent cations (e.g., Ca 2+ , Mn 2+ , Ba 2+ , Cu 2+ ) and absorbs excess wound fluid while it maintains a physiological moist wound environment [13,77,79,80]. On the other hand, shape infidelity, excessive swelling, rapid degradation rate, low mechanical properties, etc., could hinder its use in the 3D printing of wound dressings [77,79,80]. Nevertheless, modification and/or enhancement of SA by combination with different organic/inorganic materials (e.g., various polymers such as gellan gum [13], collagen, gelatin, cellulose, etc., and/or nanoparticles) can improve its performance [77,79].…”

Section: D-printed Hydrogelsmentioning

confidence: 99%

Recent Developments in 3D-(Bio)printed Hydrogels as Wound Dressings

Kammona,

Tsanaktsidou,

Kiparissides

2024

Gels

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Wound healing is a physiological process occurring after the onset of a skin lesion aiming to reconstruct the dermal barrier between the external environment and the body. Depending on the nature and duration of the healing process, wounds are classified as acute (e.g., trauma, surgical wounds) and chronic (e.g., diabetic ulcers) wounds. The latter take several months to heal or do not heal (non-healing chronic wounds), are usually prone to microbial infection and represent an important source of morbidity since they affect millions of people worldwide. Typical wound treatments comprise surgical (e.g., debridement, skin grafts/flaps) and non-surgical (e.g., topical formulations, wound dressings) methods. Modern experimental approaches include among others three dimensional (3D)-(bio)printed wound dressings. The present paper reviews recently developed 3D (bio)printed hydrogels for wound healing applications, especially focusing on the results of their in vitro and in vivo assessment. The advanced hydrogel constructs were printed using different types of bioinks (e.g., natural and/or synthetic polymers and their mixtures with biological materials) and printing methods (e.g., extrusion, digital light processing, coaxial microfluidic bioprinting, etc.) and incorporated various bioactive agents (e.g., growth factors, antibiotics, antibacterial agents, nanoparticles, etc.) and/or cells (e.g., dermal fibroblasts, keratinocytes, mesenchymal stem cells, endothelial cells, etc.).

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3D-bioprinted double-crosslinked angiogenic alginate/chondroitin sulfate patch for diabetic wound healing

Cited by 22 publications

References 37 publications

Bioinspired 3D-printed scaffold embedding DDAB-nano ZnO/nanofibrous microspheres for regenerative diabetic wound healing

Bioinspired 3D-printed scaffold embedding DDAB-nano ZnO/nanofibrous microspheres for regenerative diabetic wound healing

Natural and Synthetic Polymeric Biomaterials for Application in Wound Management

Recent Developments in 3D-(Bio)printed Hydrogels as Wound Dressings

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