3D Bioprinting as a Designer Organoid to Assess Pathological Processes in Translational Medicine

Rameshwar, Pranela; Savanur, Vibha Harindra; Etchegaray, Jean‐Pierre; Guvendiren, Murat

doi:10.2217/3dp-2021-0006

Cited by 3 publications

(4 citation statements)

References 59 publications

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“…The 3D bioprinting process is composed of cells printed in basic matrix bioinks, such as hydrogels. Bioinks are printable biomaterials used in 3D printing that are important for precision medicine due to the use of tissue organoids with diverse applications, such as drug screening (testing the efficacy and toxicity of drugs) or patient-derived xenografts [ 263 ]. Hence, the 3D bioprinting can be used to develop more physiologically relevant in vitro models for drug discovery and development [ 264 , 265 ].…”

Section: Bioengineered Organoids and 3d Bioprintingmentioning

confidence: 99%

Revolutionizing Disease Modeling: The Emergence of Organoids in Cellular Systems

Silva-Pedrosa

Salgado

Ferreira

2023

Cells

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Cellular models have created opportunities to explore the characteristics of human diseases through well-established protocols, while avoiding the ethical restrictions associated with post-mortem studies and the costs associated with researching animal models. The capability of cell reprogramming, such as induced pluripotent stem cells (iPSCs) technology, solved the complications associated with human embryonic stem cells (hESC) usage. Moreover, iPSCs made significant contributions for human medicine, such as in diagnosis, therapeutic and regenerative medicine. The two-dimensional (2D) models allowed for monolayer cellular culture in vitro; however, they were surpassed by the three-dimensional (3D) cell culture system. The 3D cell culture provides higher cell–cell contact and a multi-layered cell culture, which more closely respects cellular morphology and polarity. It is more tightly able to resemble conditions in vivo and a closer approach to the architecture of human tissues, such as human organoids. Organoids are 3D cellular structures that mimic the architecture and function of native tissues. They are generated in vitro from stem cells or differentiated cells, such as epithelial or neural cells, and are used to study organ development, disease modeling, and drug discovery. Organoids have become a powerful tool for understanding the cellular and molecular mechanisms underlying human physiology, providing new insights into the pathogenesis of cancer, metabolic diseases, and brain disorders. Although organoid technology is up-and-coming, it also has some limitations that require improvements.

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Section: Bioengineered Organoids and 3d Bioprintingmentioning

confidence: 99%

Revolutionizing Disease Modeling: The Emergence of Organoids in Cellular Systems

Silva-Pedrosa

Salgado

Ferreira

2023

Cells

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“…3-dimensional (3D) cultures is an advantage to include with 2D studies. 3D structures could be established by bioprinting or tissue organoids [ 26 ]. The BM, unlike other organs, is generally difficult to establish as an organoid or to be used in explant cultures.…”

mentioning

confidence: 99%

“…The advantage of 3D bioprinting is to design a structure that recapitulates the marrow [ 25 ]. The bioprinting can be established as a tissue with microenvironmental cells and molecules specific to the organ [ 26 ]. This would require coordinating how multiple cells and molecules are printed within a matrix that allows the survival of all cell types [ 26 ].…”

mentioning

confidence: 99%

“…The bioprinting can be established as a tissue with microenvironmental cells and molecules specific to the organ [ 26 ]. This would require coordinating how multiple cells and molecules are printed within a matrix that allows the survival of all cell types [ 26 ]. More importantly, 3D bioprinting could also mimic age since the stiffness of a microenvironment can be adjusted to the patient’s age.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Leveraging the scientific findings to develop therapeutic strategies for dormant breast cancer cells

Ferrer¹,

Kenfack²,

Petryna³

et al. 2022

Oncoscience

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Breast cancer (BC) metastasis can occur decades before clinical diagnosis. During this time, the cancer cells (BCCs) can remain dormant for decades. This type of dormancy also occurs during remission where the dormant BCCs adapt cycling quiescence within the tissue microenvironment. BC shows preference for the bone marrow (BM), resulting in poor prognosis. The BM provides a challenge due to the complex niche between the peripheral interface and endosteum. The process of dormancy begins upon entry into the marrow with the changes facilitated through crosstalk between the cancer cells and tissue niche. More importantly, dormancy can occur at any time during the disease process, including the time during treatment. This perspective discusses the challenges posed by the marrow microenvironment to develop treatment. The article discusses the complex mechanisms at each compartment within the marrow niche and the added negative issue of toxicity to the endogenous stem cells.

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Engineered organoids for biomedical applications

Roberto de Barros,

Wang,

Maity

et al. 2023

Advanced Drug Delivery Reviews

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3D Bioprinting as a Designer Organoid to Assess Pathological Processes in Translational Medicine

Cited by 3 publications

References 59 publications

Revolutionizing Disease Modeling: The Emergence of Organoids in Cellular Systems

Revolutionizing Disease Modeling: The Emergence of Organoids in Cellular Systems

Leveraging the scientific findings to develop therapeutic strategies for dormant breast cancer cells

Engineered organoids for biomedical applications

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