3D bioprinting of cells, tissues and organs

Dey, Madhuri; Özbolat, İbrahim T.

doi:10.1038/s41598-020-70086-y

Cited by 207 publications

(146 citation statements)

References 24 publications

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“…In recent years, considerable attention has been focused on the utilization of 3D bioprinting for building functional tissues, organs, and therapeutics. 22 3D bioprinting allows the control of biomaterials, biomolecules, and cells in a layer-by-layer process to create a 3D tissue construct with geometrical complexities. 30 …”

Section: Advances In 3d Bioprintingmentioning

confidence: 99%

“…Recently, 3D bioprinting has been advanced to utilize cells as building blocks to construct living tissues and organs from the ground directly. 21 , 22 The encapsulated live cells in pre-gel solutions have been utilized as bioinks to 3D print the desired tissue structures. 23 Therefore, 3D bioprinting has recently begun to be used for engineering advanced in vitro models as well.…”

Section: Introductionmentioning

confidence: 99%

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Application of 3D bioprinting in the prevention and the therapy for human diseases

Kim

Kwon

et al. 2021

Sig Transduct Target Ther

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Rapid development of vaccines and therapeutics is necessary to tackle the emergence of new pathogens and infectious diseases. To speed up the drug discovery process, the conventional development pipeline can be retooled by introducing advanced in vitro models as alternatives to conventional infectious disease models and by employing advanced technology for the production of medicine and cell/drug delivery systems. In this regard, layer-by-layer construction with a 3D bioprinting system or other technologies provides a beneficial method for developing highly biomimetic and reliable in vitro models for infectious disease research. In addition, the high flexibility and versatility of 3D bioprinting offer advantages in the effective production of vaccines, therapeutics, and relevant delivery systems. Herein, we discuss the potential of 3D bioprinting technologies for the control of infectious diseases. We also suggest that 3D bioprinting in infectious disease research and drug development could be a significant platform technology for the rapid and automated production of tissue/organ models and medicines in the near future.

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Section: Advances In 3d Bioprintingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of 3D bioprinting in the prevention and the therapy for human diseases

Kim

Kwon

et al. 2021

Sig Transduct Target Ther

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“…One way to generate 3D cell-scaffold constructs is to use 3D bioprinting technology, i.e., additive biofabrication [ 143 , 144 , 145 ]. A suitable biomaterial can be printed simultaneously, and will serve as the scaffold for the printed cells [ 146 ]. However, to 3D print muscle analogs characterized by high cell alignment and synchronous contraction, some critical barriers have to be understood and overcome.…”

Section: Bioreactors and Scaffoldingmentioning

confidence: 99%

Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

Djisalov

Knežić

Podunavac

et al. 2021

Biology

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Meat cultivation via cellular agriculture holds great promise as a method for future food production. In theory, it is an ideal way of meat production, humane to the animals and sustainable for the environment, while keeping the same taste and nutritional values as traditional meat and having additional benefits such as controlled fat content and absence of antibiotics and hormones used in the traditional meat industry. However, in practice, there is still a number of challenges, such as those associated with the upscale of cultured meat (CM). CM food safety monitoring is a necessary factor when envisioning both the regulatory compliance and consumer acceptance. To achieve this, a multidisciplinary approach is necessary. This includes extensive development of the sensitive and specific analytical devices i.e., sensors to enable reliable food safety monitoring throughout the whole future food supply chain. In addition, advanced monitoring options can help in the further optimization of the meat cultivation which may reduce the currently still high costs of production. This review presents an overview of the sensor monitoring options for the most relevant parameters of importance for meat cultivation. Examples of the various types of sensors that can potentially be used in CM production are provided and the options for their integration into bioreactors, as well as suggestions on further improvements and more advanced integration approaches. In favor of the multidisciplinary approach, we also include an overview of the bioreactor types, scaffolding options as well as imaging techniques relevant for CM research. Furthermore, we briefly present the current status of the CM research and related regulation, societal aspects and challenges to its upscaling and commercialization.

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“…Finally, one could also expect interesting results from bioprinting approaches, which allow on-demand generation of pieces of cell sheets and tissues, with flexible and precise time and dimension control [ 65 ]. Based on the large panel of available bioinks, bioprinting opens the door to a large number of possibilities recapitulating complex microenvironments, including precise regulation of the composition of various cytokines, inflammatory protein, and OS-linked species.…”

Section: New Concepts In Oxidative Stressmentioning

confidence: 99%

Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives

Chazelas

Steichen

Favreau

et al. 2021

IJMS

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Ischemia reperfusion injury is a complex process consisting of a seemingly chaotic but actually organized and compartmentalized shutdown of cell function, of which oxidative stress is a key component. Studying oxidative stress, which results in an imbalance between reactive oxygen species (ROS) production and antioxidant defense activity, is a multi-faceted issue, particularly considering the double function of ROS, assuming roles as physiological intracellular signals and as mediators of cellular component damage. Herein, we propose a comprehensive overview of the tools available to explore oxidative stress, particularly in the study of ischemia reperfusion. Applying chemistry as well as biology, we present the different models currently developed to study oxidative stress, spanning the vitro and the silico, discussing the advantages and the drawbacks of each set-up, including the issues relating to the use of in vitro hypoxia as a surrogate for ischemia. Having identified the limitations of historical models, we shall study new paradigms, including the use of stem cell-derived organoids, as a bridge between the in vitro and the in vivo comprising 3D intercellular interactions in vivo and versatile pathway investigations in vitro. We shall conclude this review by distancing ourselves from “wet” biology and reviewing the in silico, computer-based, mathematical modeling, and numerical simulation options: (a) molecular modeling with quantum chemistry and molecular dynamic algorithms, which facilitates the study of molecule-to-molecule interactions, and the integration of a compound in a dynamic environment (the plasma membrane...); (b) integrative systemic models, which can include many facets of complex mechanisms such as oxidative stress or ischemia reperfusion and help to formulate integrated predictions and to enhance understanding of dynamic interaction between pathways.

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3D bioprinting of cells, tissues and organs

Cited by 207 publications

References 24 publications

Application of 3D bioprinting in the prevention and the therapy for human diseases

Application of 3D bioprinting in the prevention and the therapy for human diseases

Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives

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