3D bioprinting of graphene oxide-incorporated cell-laden bone mimicking scaffolds for promoting scaffold fidelity, osteogenic differentiation and mineralization

Zhang, Jianhua; Eyisoylu, Hande; Qin, Xiao‐Hua; Rubert, Marina; Müller, Ralph

doi:10.1016/j.actbio.2020.12.026

Cited by 126 publications

(112 citation statements)

References 70 publications

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“…As GO fillers impart good mechanical performance and osteoconductivity, they have attracted considerable attention for their application as nanocomposites for bone tissue engineering [ 25 , 26 ]. Angiogenesis precedes osteogenesis, and a large number of osteoprogenitors are recruited and distributed around H-type blood vessels.…”

Section: Resultsmentioning

confidence: 99%

Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid

Liu

Wei

et al. 2022

Bioactive Materials

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Graphene oxide (GO) exhibits good mechanical and physicochemical characteristics and has extensive application prospects in bone tissue engineering. However, its effect on angiogenesis is unclear, and its potential toxic effects are heavily disputed. Herein, we found that nanographene oxide (NGO) synthesized by one-step water electrolytic oxidation is smaller and shows superior biocompatibility. Moreover, NGO significantly enhanced angiogenesis in calvarial bone defect areas in vivo, providing a good microenvironment for bone regeneration. Endothelial tip cell differentiation is an important step in the initiation of angiogenesis. We verified that NGO activates endothelial tip cells by coupling with lysophosphatidic acid (LPA) in serum via strong hydrogen bonding interactions, which has not been reported. In addition, the mechanism by which NGO promotes angiogenesis was systematically studied. NGO-coupled LPA activates LPAR6 and facilitates the formation of migratory tip cells via Hippo/Yes-associated protein (YAP) independent of reactive oxygen species (ROS) stimulation or additional complex modifications. These results provide an effective strategy for the application of electrochemically derived NGO and more insight into NGO-mediated angiogenesis.

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Section: Resultsmentioning

confidence: 99%

Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid

Liu

Wei

et al. 2022

Bioactive Materials

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“…Bioactive inks containing 0.5 mg/mL graphene oxide illustrated the highest ALP activity, calcium deposition, as well as expression of multiple osteogenic markers in addition to acceptable printability and shape fidelity. A group of researchers investigated the effect of different concentrations of graphene oxide to alginate-gelatin bioactive ink on osteogenic differentiation of hMSCs as well as printability and shape fidelity [ 85 ]. The cell-laden scaffolds with 1 mg/mL graphene oxide concentration in bioreactor illustrated better gene expression and ECM mineralization after 42 days than alginate-gelatin scaffolds ( Figure 5 B).…”

Section: Bioactive Inksmentioning

confidence: 99%

“…Compared to that of CTR (blank control) and TCP groups, Micro-CT analysis of defect space exhibited a distinctly greater level of bone regeneration in SPS group (reproduced content is open access) [ 82 ]. ( B ) Light microscopy images of 3D bioprinted cell-laden GO scaffolds cultured in osteogenic media for 1, 7, and 42 days (reproduced content is open access) [ 85 ].…”

Section: Bioactive Inksmentioning

confidence: 99%

Bioactive Inks Development for Osteochondral Tissue Engineering: A Mini-Review

Bakhtiary

Liu

Ghorbani

2021

Gels

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Nowadays, a prevalent joint disease affecting both cartilage and subchondral bone is osteoarthritis. Osteochondral tissue, a complex tissue unit, exhibited limited self-renewal potential. Furthermore, its gradient properties, including mechanical property, bio-compositions, and cellular behaviors, present a challenge in repairing and regenerating damaged osteochondral tissues. Here, tissue engineering and translational medicine development using bioprinting technology provided a promising strategy for osteochondral tissue repair. In this regard, personalized stratified scaffolds, which play an influential role in osteochondral regeneration, can provide potential treatment options in early-stage osteoarthritis to delay or avoid the use of joint replacements. Accordingly, bioactive scaffolds with possible integration with surrounding tissue and controlling inflammatory responses have promising future tissue engineering perspectives. This minireview focuses on introducing biologically active inks for bioprinting the hierarchical scaffolds, containing growth factors and bioactive materials for 3D printing of regenerative osteochondral substitutes.

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“…A human MSC-laden GO/ALG/GLT composite bioink was prepared to form 3D bone-mimicking scaffolds using a 3D bioprinting technique. The GO composite bioinks containing higher GO concentrations (0.5, 1, and 2 mg/mL) improved the bioprintability, scaffold fidelity, compressive modulus, cell proliferation, osteogenic differentiation, and extracellular matrix mineralization compared to the pure ALG/GLT system, while the bioink with GO concentration 1 mg/mL was the optimal filler [156]. A scaffold composed of mesoporous bioactive glasses and GO was investigated for local angiogenesis and bone healing.…”

Section: Graphene-based Materialsmentioning

confidence: 99%

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

Jampílek

Plachá

2021

Pharmaceutics

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Since the worldwide incidence of bone disorders and cartilage damage has been increasing and traditional therapy has reached its limits, nanomaterials can provide a new strategy in the regeneration of bones and cartilage. The nanoscale modifies the properties of materials, and many of the recently prepared nanocomposites can be used in tissue engineering as scaffolds for the development of biomimetic materials involved in the repair and healing of damaged tissues and organs. In addition, some nanomaterials represent a noteworthy alternative for treatment and alleviating inflammation or infections caused by microbial pathogens. On the other hand, some nanomaterials induce inflammation processes, especially by the generation of reactive oxygen species. Therefore, it is necessary to know and understand their effects in living systems and use surface modifications to prevent these negative effects. This contribution is focused on nanostructured scaffolds, providing a closer structural support approximation to native tissue architecture for cells and regulating cell proliferation, differentiation, and migration, which results in cartilage and bone healing and regeneration.

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3D bioprinting of graphene oxide-incorporated cell-laden bone mimicking scaffolds for promoting scaffold fidelity, osteogenic differentiation and mineralization

Cited by 126 publications

References 70 publications

Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid

Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid

Bioactive Inks Development for Osteochondral Tissue Engineering: A Mini-Review

Advances in Use of Nanomaterials for Musculoskeletal Regeneration

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