3D Cell-Printed Hypoxic Cancer-on-a-Chip for Recapitulating Pathologic Progression of Solid Cancer

Park, Wonbin; Bae, Mihyeon; Hwang, Minseon; Jang, Jinah; Cho, Dong‐Woo; Yi, Hee

doi:10.3791/61945

Cited by 7 publications

(6 citation statements)

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“…The chip would be able to induce hypoxia and stimulate malignancy with the expression of cancer markers. Thus, this chip helps to create a solid-cancer-mimetic microphysiology to bridge the gap between the in vitro and in vivo models for cancer research [191]. A 3D-printed polymeric lab-on-a-chip was developed to tune the intrinsic functionalities by using DLP technology.…”

Section: Cancer-on-a-chip and 3d Printingmentioning

confidence: 99%

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Safhi

2022

Pharmaceuticals

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Three-dimensional (3D) printing is a technique where the products are printed layer-by-layer via a series of cross-sectional slices with the exact deposition of different cell types and biomaterials based on computer-aided design software. Three-dimensional printing can be divided into several approaches, such as extrusion-based printing, laser-induced forward transfer-based printing systems, and so on. Bio-ink is a crucial tool necessary for the fabrication of the 3D construct of living tissue in order to mimic the native tissue/cells using 3D printing technology. The formation of 3D software helps in the development of novel drug delivery systems with drug screening potential, as well as 3D constructs of tumor models. Additionally, several complex structures of inner tissues like stroma and channels of different sizes are printed through 3D printing techniques. Three-dimensional printing technology could also be used to develop therapy training simulators for educational purposes so that learners can practice complex surgical procedures. The fabrication of implantable medical devices using 3D printing technology with less risk of infections is receiving increased attention recently. A Cancer-on-a-chip is a microfluidic device that recreates tumor physiology and allows for a continuous supply of nutrients or therapeutic compounds. In this review, based on the recent literature, we have discussed various printing methods for 3D printing and types of bio-inks, and provided information on how 3D printing plays a crucial role in cancer management.

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Section: Cancer-on-a-chip and 3d Printingmentioning

confidence: 99%

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Safhi

2022

Pharmaceuticals

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“…Compared to single ECMs (e.g. collagen or fibronectin), utilizing dECMs derived from normal or diseased tissue could contribute to mimicking a reliable microenvironment because they could reflect the structural and compositional disorganization of ECM during disease progression ( 150 , 151 ). Interestingly, Alfano et al ( 152 ) showed different intestinal models using three types of dECM substrates derived from healthy, perilesional, and colorectal carcinoma (CRC) human tissue.…”

Section: The Usefulness Of Microenvironmental Cues In Gut Modelsmentioning

confidence: 99%

Recent advances in biofabricated gut models to understand the gut-brain axis in neurological diseases

Han

Jang²

2022

Front. Med. Technol.

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Increasing evidence has accumulated that gut microbiome dysbiosis could be linked to neurological diseases, including both neurodegenerative and psychiatric diseases. With the high prevalence of neurological diseases, there is an urgent need to elucidate the underlying mechanisms between the microbiome, gut, and brain. However, the standardized aniikmal models for these studies have critical disadvantages for their translation into clinical application, such as limited physiological relevance due to interspecies differences and difficulty interpreting causality from complex systemic interactions. Therefore, alternative in vitro gut–brain axis models are highly required to understand their related pathophysiology and set novel therapeutic strategies. In this review, we outline state-of-the-art biofabrication technologies for modeling in vitro human intestines. Existing 3D gut models are categorized according to their topographical and anatomical similarities to the native gut. In addition, we deliberate future research directions to develop more functional in vitro intestinal models to study the gut–brain axis in neurological diseases rather than simply recreating the morphology.

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“…In addition, a 3D culture enables the observation of the spatiotemporal dynamics of nature, allowing the reproduction of metabolic exchanges and regulation of cell fate. For example, hypoxia is a crucial driver of cancer development and induces genomic stability and tumorigenesis [ 21 ]. In an attempt to generate central hypoxia, Yi et al reproduced a radial oxygen gradient using a 3D gas permeable barrier with ECM containing brain cancer cells and endothelial cells, which is poorly reflected in a 2D culture platform [ 22 ].…”

Section: Requirements For Advanced Pharmaceutical Techniquesmentioning

confidence: 99%

3D Printing of Pharmaceutical Application: Drug Screening and Drug Delivery

et al. 2021

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Advances in three-dimensional (3D) printing techniques and the development of tailored biomaterials have facilitated the precise fabrication of biological components and complex 3D geometrics over the past few decades. Moreover, the notable growth of 3D printing has facilitated pharmaceutical applications, enabling the development of customized drug screening and drug delivery systems for individual patients, breaking away from conventional approaches that primarily rely on transgenic animal experiments and mass production. This review provides an extensive overview of 3D printing research applied to drug screening and drug delivery systems that represent pharmaceutical applications. We classify several elements required by each application for advanced pharmaceutical techniques and briefly describe state-of-the-art 3D printing technology consisting of cells, bioinks, and printing strategies that satisfy requirements. Furthermore, we discuss the limitations of traditional approaches by providing concrete examples of drug screening (organoid, organ-on-a-chip, and tissue/organ equivalent) and drug delivery systems (oral/vaginal/rectal and transdermal/surgical drug delivery), followed by the introduction of recent pharmaceutical investigations using 3D printing-based strategies to overcome these challenges.

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3D Cell-Printed Hypoxic Cancer-on-a-Chip for Recapitulating Pathologic Progression of Solid Cancer

Cited by 7 publications

References 0 publications

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Recent advances in biofabricated gut models to understand the gut-brain axis in neurological diseases

3D Printing of Pharmaceutical Application: Drug Screening and Drug Delivery

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