3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

Abstract

The development of artificial tissue/organs with the functional maturity of their native equivalents is one of the long-awaited panaceas for the medical and pharmaceutical industries. Advanced 3D cell-printing technology and various functional bioinks are promising technologies in the field of tissue engineering that have enabled the fabrication of complex 3D living tissue/organs. Various requirements for these tissues, including a complex and large-volume structure, tissue-specific microenvironments, and functional vasculatures, have been addressed to develop engineered tissue/organs with native relevance. Functional tissue/organ constructs have been developed that satisfy such criteria and may facilitate both in vivo replenishment of damaged tissue and the development of reliable in vitro testing platforms for drug development. This review describes key developments in technologies and materials for engineering 3D cell-printed constructs for therapeutic and drug testing applications.

Effect of Photobiomodulation in Suppression of Oxidative Stress on Retinal Pigment Epithelium

“…Recently, various kinds of in vitro models have been widely used in the pharmaceutical industry for the drug development and research field for understanding the pathogenesis of the disease [ 36 , 37 ]. In particular, advanced tissue-specific ECM materials have been accelerated to develop an organ model by recapitulating the microenvironment of the target organ.…”

Maturation and Protection Effect of Retinal Tissue-Derived Bioink for 3D Cell Printing Technology

“…Recently, 3D printing and tissue-specific bioink have been widely used in tissue engineering, including regeneration medicine and drug-testing models [ 21 ]. The advanced 3D printing system could simultaneously print various materials, including synthetic and natural polymers.…”

“…Recently, 3D cell printing technology has been widely used to fabricate printed cell constructs for use in regeneration medicine and to establish in vitro models due to its ability to precisely deposit biomaterials and living cells according to their native equivalents [ 21 , 22 , 23 ]. Furthermore, advancements in bioink technology have also contributed to the recapitulation of the natural environment of the tissues [ 24 , 25 , 26 ].…”

Fluidic circuit board with modular sensor and valves enables stand-alone, tubeless microfluidic flow control in organs-on-chips

“…4 Organs-on-chips allow biologists to perform assays of tissue functionality that are impossible to perform in common cell culture hardware such as well plates. 5,6 There is a wide range of applications of organs-on-chips in fields like pharmacology, toxicology, stem cell biology and biomedical science. 7…”