3D In Vivo Optical Skin Imaging for Intense Pulsed Light and Fractional Ablative Resurfacing of Photodamaged Skin

Clementoni, Matteo Tretti; Lavagno, Rosalia; Catenacci, Maximilian; Kantor, Roman; Mariotto, Guido; Shvets, I. V.

doi:10.1016/j.fsc.2011.07.014

Cited by 9 publications

(10 citation statements)

References 27 publications

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“…Skin topography and distribution of melanin were measured in vivo with the Antera 3D imaging system (Miravex) consisting of a handheld imaging device and a connected laptop with preinstalled proprietary software . The imaging technique of the Antera 3D allows three‐dimensional imaging of partially diffusive and diffusive surfaces.…”

Section: Methodsmentioning

confidence: 99%

Fractional carbon dioxide laser resurfacing of rhytides and photoageing: a prospective study using profilometric analysis

et al. 2014

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Section: Methodsmentioning

confidence: 99%

Fractional carbon dioxide laser resurfacing of rhytides and photoageing: a prospective study using profilometric analysis

et al. 2014

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“…Thermal denaturation take place at 63-64 • C, and can occur over a range of temperatures and pulse durations with observable changes in staining and structural collagen fibers (Goldberg, 2008). There are clinical reports of improvement and some histological studies (Clementoni et al, 2011;El-Domyati et al, 2011;Omi, 2012;Kim et al, 2012) but to date, no data are clear on the effects of IPL near infrared on human skin cells and the associated mechanism.…”

Section: Background and Objectivesmentioning

confidence: 99%

Increased fibroblast proliferation and activity after applying intense pulsed light 800–1200nm

Cuerda-Galindo

Dı́az-Gil

Palomar-Gallego

et al. 2015

Annals of Anatomy - Anatomischer Anzeiger

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“…Until now, the proposed theory for rejuvenation with IPL systems is based on water heating and fiber degradation and that more heating and protein denaturalization leads to better clinical results [9,10]. With our study in vitro, we can hypothesize that biochemical mechanism can be, despite water heating, the direct stimuli of fibroblast by light which result in an increase of mRNA and synthesis of collagen and ECM proteins.…”

Section: Discussionmentioning

confidence: 73%

“…Thermal denaturation take place at 63 to 64°C and can occur over a range of temperatures and pulse durations with observable changes in staining and structural collagen fibers [8]. There are clinical reports of improvement and some histological studies using different wavelengths [10,11] although some authors argue that collagen denaturation is insufficient as a sole and direct corollary for effective treatment [12]. To date, scarce data are reported on the effects of IPL on human skin cells and the associated mechanism [13].…”

Section: Introductionmentioning

confidence: 99%

Intense pulsed light induces synthesis of dermal extracellular proteins in vitro

et al. 2015

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Intense pulsed light (IPL) devices have been shown to be highly effective for the skin rejuvenation. In our study, we try to elucidate effects of IPL in fibroblast proliferation, in gene expression, and in extracellular matrix protein production. 1BR3G human skin fibroblasts were used to test the effects of an IPL device (MiniSilk FT, Deka®). Fibroblasts were divided into three groups: group 1 was irradiated with filter 800-1200 nm (frequency 10 Hz, 15 s, fluence 60.1 J/cm) twice; group 2 was irradiated with filter 550-1200 nm (double pulse 5 ms + 5 ms, delay 10 ms, fluence 13 J/cm2) twice; and group 3 was irradiated with filter 550-1200 nm (frequency 10 Hz, 15 s, fluence 60.1 J/cm2) twice. To determine changes in gene expression, messenger RNA (mRNA) levels for collagen types I and III and metalloproteinase 1 (MMP-1) were performed 48 h after irradiation. To determine changes in hyaluronic acid, versican, and decorin, mRNA and ELISA tests were performed after 48 h of treatment. In addition to this, a Picro-Sirius red staining for collagen was made. The study showed an increase of mRNA and hyaluronic acid, decorin, and versican production. With RT-PCR assays, an increase mRNA for collagen type I, type III, and MMP-1 was observed. Collagen and hyaluronic synthesis was increased in all groups with no differences among them, while decorin and versican synthesis was higher in those groups irradiated with 550-1200-nm filters with no dependence of type pulse or total energy dose. IPL applied in vitro cultured cells increases fibroblasts activity. Synthesis of extracellular proteins seems to be produced more specifically in determined wavelengths, which could demonstrate a biochemical mechanism light depending.

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3D In Vivo Optical Skin Imaging for Intense Pulsed Light and Fractional Ablative Resurfacing of Photodamaged Skin

Cited by 9 publications

References 27 publications

Fractional carbon dioxide laser resurfacing of rhytides and photoageing: a prospective study using profilometric analysis

Fractional carbon dioxide laser resurfacing of rhytides and photoageing: a prospective study using profilometric analysis

Increased fibroblast proliferation and activity after applying intense pulsed light 800–1200nm

Intense pulsed light induces synthesis of dermal extracellular proteins in vitro

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