3D neural tissue models: From spheroids to bioprinting

Zhuang, Pei; Sun, Alfred Xuyang; An, Jia; Chua, Chee Kai; Chew, Sing Yian

doi:10.1016/j.biomaterials.2017.10.002

Cited by 234 publications

(210 citation statements)

References 227 publications

(312 reference statements)

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“…Lasers are increasingly used in MJ, examples are laser jetting (Zenou, Sa'ar, and Kotler 2015) and laser-assisted bioprinting (Yu et al 2019;Zhuang et al 2018). A typical laser-assisted bioprinting system consists of a pulsed laser beam with a focusing device, a donor slide and a collector slide facing the ribbon.…”

Section: Editorialmentioning

confidence: 99%

Editorial

Chua

2020

Virtual and Physical Prototyping

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Section: Editorialmentioning

confidence: 99%

Editorial

Chua

2020

Virtual and Physical Prototyping

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“…[30,31] One appropriate application of these spheroids is organoids. [32][33][34][35][36][37] To investigate the possibility of resemble organoids by our method, we used human umbilical vein endothelial cell (HUVECs) and mesenchymal stem cell (MSC) to establish a complicated coculture microenvironment. The main purpose is to realize osteogenesis and angiogenesis in this artificial sphere shaped organoids.…”

Section: Doi: 101002/smll201802630mentioning

confidence: 99%

Airflow‐Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle

Zhao

Chen

Shao

et al. 2018

Small

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Hydrogel microspheroids are widely used in tissue engineering, such as injection therapy and 3D cell culture, and among which, heterogeneous microspheroids are drawing much attention as a promising tool to carry multiple cell types in separated phases. However, it is still a big challenge to fabricate heterogeneous microspheroids that can reconstruct built-up tissues' microarchitecture with excellent resolution and spatial organization in limited sizes. Here, a novel airflow-assisted 3D bioprinting method is reported, which can print versatile spiral microarchitectures inside the microspheroids, permitting one-step bioprinting of fascinating hydrogel structures, such as the spherical helix, rose, and saddle. A microfluidic nozzle is developed to improve the capability of intricate cell encapsulation with heterotypic contact. Complex structures, such as a rose, Tai chi pattern, and single cell line can be easily printed in spheroids. The theoretical model during printing is established and process parameters are systematically investigated. As a demonstration, a human multicellular organoid of spirally vascularized ossification is reconstructed with this method, which shows that it is a powerful tool to build mini tissues on microspheroids.

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“…These failures occur despite many candidate therapeutics having efficacy in various mouse models. As such, there has been a growing interest in developing human in vitro models for studying neurodegeneration [3][4][5] and reducing the attrition rate in therapeutic development.…”

Section: Introductionmentioning

confidence: 99%

“…Matrigel is also the sole ECM utilized for more recently developed hPSC-derived brain organoids [8,9] , where the ECM scaffold supports the self-organization of the neuroepithelium to induce neuroepithelial buds and facilitates growth by providing a physical structure for cells to attach to and grow [10] . Other natural and synthetic materials have been developed for extended culture of hPSC-derived neural progenitor cells (NPCs) and neurons, including silk, collagen, hyaluronic acid (HA), elastin-like peptides, and polyethylene glycol (PEG) [5,[11][12][13][14][15] . As these materials all allow for diffusion of essential nutrients and morphogens throughout the tissue constructs, they can be used to maintain NPCs and neuronal cultures for extended studies of differentiation and maturation, including axon formation, growth, and pruning [12,16,17] .…”

Section: Introductionmentioning

confidence: 99%

Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks

O’Grady

Balotin

Bosworth

et al. 2019

Preprint

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In vitro models of the human central nervous system (CNS), particularly those derived from induced pluripotent stem cells (iPSCs), are becoming increasingly recognized as useful complements to animal models for studying neurological diseases and developing therapeutic strategies. However, current 3D CNS models suffer from deficits that limit their research utility. Notably, it remains difficult to drive iPSC-derived neurons to a mature and synaptically connected state. Moreover, the most common extracellular matrices (ECMs) used to fabricate 3D CNS models are either difficult to pattern into complex structures due to their mechanical properties or lack appropriate bioinstructive cues. Here, we describe the functionalization of gelatin methacrylate (GelMA) with an N-cadherin extracellular peptide epitope to create a biomaterial termed GelMA-Cad. After photopolymerization, GelMA-Cad forms soft hydrogels that can maintain patterned architectures. The N-cadherin functionality promotes survival and maturation of iPSC-derived glutamatergic neurons into synaptically connected networks as determined by viral tracing and electrophysiology. Immunostaining reveals a pronounced increase in presynaptic and postsynaptic marker expression in GelMA-Cad relative to Matrigel, as well as extensive co-localization of these markers, thus highlighting the biological activity of the N-cadherin peptide. Overall, given its ability to enhance iPSC-derived neuron maturity and connectivity, GelMA-Cad should be broadly useful for in vitro studies of neural circuitry in health and disease.

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3D neural tissue models: From spheroids to bioprinting

Cited by 234 publications

References 227 publications

Editorial

Editorial

Airflow‐Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle

Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks

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