3D-printable chitosan/silk fibroin/cellulose nanoparticle scaffolds for bone regeneration via M2 macrophage polarization

Patel, Dinesh Kumar; Dutta, Sayan Deb; Hexiu, Jin; Ganguly, Keya

doi:10.1016/j.carbpol.2021.119077

Cited by 75 publications

(38 citation statements)

References 80 publications

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“…For example, reduced scale honeycomb-like TiO2 structures were shown to facilitate macrophage filopodia formation to reinforce its polarization toward M2, which further triggered osteogenic differentiation of bone mesenchymal stem cells (BMSCs). [14] Many hydrogels, such as GelMA hydrogel, [15] carboxymethyl chitosan/poly(ethylene glycol) diacrylate hydrogel, [16] glycopeptide hydrogel, [17] and chitosan hydrogel, [18] have also been modified and used to promote bone repair through facilitating M2 macrophage polarization. However, as the pro-regenerative role of M2 macrophages was overly emphasized in these efforts, the positive effect of M1 macrophages in terms of damage-associated debris clearance, immune cell recruitment, and angiogenic induction was underestimated, which may severely compromise the effectiveness of such immunomodulatory bone healing strategies.…”

Section: Introductionmentioning

confidence: 99%

Remotely Temporal Scheduled Macrophage Phenotypic Transition Enables Optimized Immunomodulatory Bone Regeneration

Huang

et al. 2022

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Precise timing of macrophage polarization plays a pivotal role in immunomodulation of tissue regeneration, yet most studies mainly focus on M2 macrophages for their anti‐inflammatory and regenerative effects while the essential proinflammatory role of the M1 phenotype on the early inflammation stage is largely underestimated. Herein, a superparamagnetic hydrogel capable of timely controlling macrophage polarization is constructed by grafting superparamagnetic nanoparticles on collagen nanofibers. The magnetic responsive hydrogel network enables efficient polarization of encapsulated macrophage to the M2 phenotype through the podosome/Rho/ROCK mechanical pathway in response to static magnetic field (MF) as needed. Taking advantage of remote accessibility of magnetic field together with the superparamagnetic hydrogels, a temporal engineered M1 to M2 transition course preserving the essential role of M1 at the early stage of tissue healing, as well as enhancing the prohealing effect of M2 at the middle/late stages is established via delayed MF switch. Such precise timing of macrophage polarization matching the regenerative process of injured tissue eventually leads to optimized immunomodulatory bone healing in vivo. Overall, this study offers a remotely time‐scheduled approach for macrophage polarization, which enables precise manipulation of inflammation progression during tissue healing.

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Section: Introductionmentioning

confidence: 99%

Remotely Temporal Scheduled Macrophage Phenotypic Transition Enables Optimized Immunomodulatory Bone Regeneration

Huang

et al. 2022

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“…This was also reflected in the porosity of the printed structures. We obtained negligible differences in the pore uniformity (Figure S10c) of pure 6-Alg/4-Gel and its composite bioinks after 3D printing and CaCl 2 cross-linking, demonstrating that all formulated bioinks had excellent print stability, which is crucial for tissue engineering. , …”

Section: Resultsmentioning

confidence: 99%

Bioengineered Lab-Grown Meat-like Constructs through 3D Bioprinting of Antioxidative Protein Hydrolysates

Dutta

Ganguly

Jeong

et al. 2022

ACS Appl. Mater. Interfaces

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Lab-grown bovine meat analogues are emerging alternatives to animal sacrifices for cultured meat production. The most challenging aspect of the production process is the rapid proliferation of cells and establishment of the desired 3D structure for mass production. In this study, we developed a direct ink writing-based 3D-bioprinted meat culture platform composed of 6% (w/v) alginate and 4% (w/v) gelatin (Alg/Gel)-based hydrogel scaffolds supplemented with naturally derived protein hydrolysates (PHs; 10%) from highly nutritive plants (soybean, pigeon pea, and wheat), and some selected edible insects (beetles, crickets, and mealworms) on in vitro proliferation of bovine myosatellite cells (bMSCs) extracted from fresh meat samples. The developed bioink exhibited excellent shear-thinning behavior (n < 1) and mechanical stability during 3D bioprinting. Commercial proteases (Alcalase, Neutrase, and Flavourzyme) were used for protein hydrolysis. The resulting hydrolysates exhibited lower-molecular-weight bands (12–50 kDa) than those of crude isolates (55–160 kDa), as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The degree of hydrolysis was higher in the presence of Alcalase for both plant (34%) and insect (62%) PHs than other enzymes. The 3D-printed hydrogel scaffolds displayed excellent bioactivity and stability after 7 days of incubation. The developed prototype structure (pepperoni meat, 20 × 20 × 5 mm) provided a highly stable, nutritious, and mechanically strong structure that supported the rapid proliferation of myoblasts in a low-serum environment during the entire culture period. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay enhanced the free radical reduction of Alcalase- and Neutrase-treated PHs. Furthermore, the bioprinted bMSCs displayed early myogenesis (desmin and Pax7) in the presence of PHs, suggesting its role in bMSC differentiation. In conclusion, we developed a 3D bioprinted and bioactive meat culture platform using Alg/Gel/PHs as a printable and edible component for the mass production of cultured meat.

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“…Another novel method for fabricating artificial scaffolds is 3D printing. Its use involves bio-printers, needles, bio-inks, and design software [ 79 ]. A 3D model was generated using computer-aided design (CAD) software for conversion to STL format.…”

Section: Biofabrication Approaches Used To Develop Artificial Scaffoldsmentioning

confidence: 99%

“…Advantages: probably the most attractive technique in terms of micro-architecture, allows the use of a wide range of biomaterials, and excellent controllability over structural properties such as porosity, pore size, and pore interconnectivity [ 79 ];…”

Section: Biofabrication Approaches Used To Develop Artificial Scaffoldsmentioning

confidence: 99%

Artificial Scaffolds in Cardiac Tissue Engineering

Roacho-Pérez

Garza-Treviño

Moncada‐Saucedo

et al. 2022

Life

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Cardiovascular diseases are a leading cause of death worldwide. Current treatments directed at heart repair have several disadvantages, such as a lack of donors for heart transplantation or non-bioactive inert materials for replacing damaged tissue. Because of the natural lack of regeneration of cardiomyocytes, new treatment strategies involve stimulating heart tissue regeneration. The basic three elements of cardiac tissue engineering (cells, growth factors, and scaffolds) are described in this review, with a highlight on the role of artificial scaffolds. Scaffolds for cardiac tissue engineering are tridimensional porous structures that imitate the extracellular heart matrix, with the ability to promote cell adhesion, migration, differentiation, and proliferation. In the heart, there is an important requirement to provide scaffold cellular attachment, but scaffolds also need to permit mechanical contractility and electrical conductivity. For researchers working in cardiac tissue engineering, there is an important need to choose an adequate artificial scaffold biofabrication technique, as well as the ideal biocompatible biodegradable biomaterial for scaffold construction. Finally, there are many suitable options for researchers to obtain scaffolds that promote cell–electrical interactions and tissue repair, reaching the goal of cardiac tissue engineering.

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3D-printable chitosan/silk fibroin/cellulose nanoparticle scaffolds for bone regeneration via M2 macrophage polarization

Cited by 75 publications

References 80 publications

Remotely Temporal Scheduled Macrophage Phenotypic Transition Enables Optimized Immunomodulatory Bone Regeneration

Remotely Temporal Scheduled Macrophage Phenotypic Transition Enables Optimized Immunomodulatory Bone Regeneration

Bioengineered Lab-Grown Meat-like Constructs through 3D Bioprinting of Antioxidative Protein Hydrolysates

Artificial Scaffolds in Cardiac Tissue Engineering

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