3D-printed bioanalytical devices

Bishop, Gregory W.; Satterwhite-Warden, Jennifer E.; Kadimisetty, Karteek; Rusling, James F.

doi:10.1088/0957-4484/27/28/284002

Cited by 58 publications

(40 citation statements)

References 49 publications

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“…The possibility of 3D printing (also known as additive manufacturing (AM)) bespoke biologically receptive parts, has driven the increasing application of AM technologies in biological systems. Techniques such as fused deposition modeling (FDM), photopolymerization processes such as stereolithography (SL) and PolyJet printing, in addition to powder‐based particle consolidation laser sintering (LS) technology have all previously been utilized to provide devices/scaffolds for bioengineering . However, the numerous advantages of AM, including design freedom and rapid production without molds or tooling, are currently offset by a lack of fully characterized biocompatible materials …”

Section: Introductionmentioning

confidence: 99%

Feasibility and Biocompatibility of 3D‐Printed Photopolymerized and Laser Sintered Polymers for Neuronal, Myogenic, and Hepatic Cell Types

Rimington

Capel

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et al. 2018

Macromolecular Bioscience

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The integration of additive manufacturing (AM) technology within biological systems holds significant potential, specifically when refining the methods utilized for the creation of in vitro models. Therefore, examination of cellular interaction with the physical/physicochemical properties of 3D-printed polymers is critically important. In this work, skeletal muscle (C C ), neuronal (SH-SY5Y) and hepatic (HepG2) cell lines are utilized to ascertain critical evidence of cellular behavior in response to 3D-printed candidate polymers: Clear-FL (stereolithography, SL), PA-12 (laser sintering, LS), and VeroClear (PolyJet). This research outlines initial critical evidence for a framework of polymer/AM process selection when 3D printing biologically receptive scaffolds, derived from industry standard, commercially available AM instrumentation. C C , SH-SY5Y, and HepG2 cells favor LS polymer PA-12 for applications in which cellular adherence is necessitated. However, cell type specific responses are evident when cultured in the chemical leachate of photopolymers (Clear-FL and VeroClear). With the increasing prevalence of 3D-printed biointerfaces, the development of rigorous cell type specific biocompatibility data is imperative. Supplementing the currently limited database of functional 3D-printed biomaterials affords the opportunity for experiment-specific AM process and polymer selection, dependent on biological application and intricacy of design features required.

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Section: Introductionmentioning

confidence: 99%

Feasibility and Biocompatibility of 3D‐Printed Photopolymerized and Laser Sintered Polymers for Neuronal, Myogenic, and Hepatic Cell Types

Rimington

Capel

Player

et al. 2018

Macromolecular Bioscience

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“…It has been used to print fluidic devices that detect pathogenic bacteria, biomedical markers, food allergens, and heavy metals, as well as in nanoparticle and chemical synthesis. 5,6 3-D printed flow cells were designed for microdroplet generation, electrochemical sensing, and microfluidic devices. 7 We used desktop 3-D printers to develop devices for flow injection amperometry, flow cells to measure ECL, 8 and microfluidic immunoarrays to detect cancer biomarker proteins.…”

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confidence: 99%

Automated 3-D Printed Arrays to Evaluate Genotoxic Chemistry: E-Cigarettes and Water Samples

2017

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A novel, automated, low cost, three-dimensional (3-D) printed microfluidic array was developed to detect DNA damage from metabolites of chemicals in environmental samples. The electrochemiluminescent (ECL) detection platform incorporates layer-by-layer (LbL) assembled films of microsomal enzymes, DNA and an ECL-emitting ruthenium metallopolymer in ∼10 nm deep microwells. Liquid samples are introduced into the array, metabolized by the human enzymes, products react with DNA if possible, and DNA damage is detected by ECL with a camera. Measurements of relative DNA damage by the array assess the genotoxic potential of the samples. The array analyzes three samples simultaneously in 5 min. Measurement of cigarette and e-cigarette smoke extracts and polluted water samples was used to establish proof of concept. Potentially genotoxic reactions from e- cigarette vapor similar to smoke from conventional cigarettes were demonstrated. Untreated wastewater showed a high genotoxic potential compared to negligible values for treated wastewater from a pollution control treatment plant. Reactivity of chemicals known to produce high rates of metabolite-related DNA damage were measured, and array results for environmental samples were expressed in terms of equivalent responses from these standards to assess severity of possible DNA damage. Genotoxic assessment of wastewater samples during processing also highlighted future on-site monitoring applications.

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“…The manufacturing of microfluidic devices by 3D‐printing technologies has been extensively explored mainly for bioanalytical analysis , due to their capacity of processing small volumes of sample and the possibility to design personalized devices for each application . Despite the remarkable advantages of these devices, their performance depends on a suitable flow control system, which can be a syringe pump, a pressure controller, valves, capillary or vacuum.…”

Section: Introductionmentioning

confidence: 99%

3D‐printed Portable Platform for Mechanized Handling and Injection of Microvolumes Coupled to Electrochemical Detection

et al. 2019

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We present a low‐cost mechanized system fabricated using fused deposition modelling 3D‐printing technology to manipulate microvolumes and perform injections on an electrochemical cell in wall‐jet configuration. As a proof‐of‐concept, the amperometric detection of paracetamol (model analyte) on a screen‐printed electrode using 0.5 μL aliquots resulted in highly reproducible responses (RSD <3 %). Moreover, handling of microliter aliquots of butylhydroxytoluene (phenolic antioxidant) and 2,2‐diphenyl‐2‐picrylhydrazyl (DPPH) to promote the radical‐scavenging reaction to determine antioxidant capacity by electrochemical detection of residual DPPH was demonstrated (time‐controlled reaction). A final application of the system was devoted to the analysis of cocaine and a common adulterant found in seized samples. The mechanized 3D‐printed analytical platform is capable to execute diverse sample preparation steps on board by handling microliter aliquots and subsequent electrochemical detection. 3D‐printing technology enabled the fabrication of a versatile and low‐cost (

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3D-printed bioanalytical devices

Cited by 58 publications

References 49 publications

Feasibility and Biocompatibility of 3D‐Printed Photopolymerized and Laser Sintered Polymers for Neuronal, Myogenic, and Hepatic Cell Types

Feasibility and Biocompatibility of 3D‐Printed Photopolymerized and Laser Sintered Polymers for Neuronal, Myogenic, and Hepatic Cell Types

Automated 3-D Printed Arrays to Evaluate Genotoxic Chemistry: E-Cigarettes and Water Samples

3D‐printed Portable Platform for Mechanized Handling and Injection of Microvolumes Coupled to Electrochemical Detection

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