3D printed collagen structures at low concentrations supported by jammed microgels

Zhang, Yifan; Ellison, Sarah; Duraivel, S.; Morley, Cameron; Taylor, Curtis R.; Angelini, Thomas E.

doi:10.1016/j.bprint.2020.e00121

Cited by 40 publications

(29 citation statements)

References 32 publications

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“…[27] Finally, values such as thixotropic response time, which are not captured in theoretical simulations, may play a key role in printing outcomes. [12,23,34] While outside the scope of this work, future studies may explore more complex models that incorporate some of these features.…”

Section: Impact Of Print Speed On Printed Filamentsmentioning

confidence: 99%

“…Carbopol refers to a group of commercially available polyacrylic acid-based microgel formulations (i.e., granular suspension media with spherical microgels of diameter < 7 μm), [6] which are widely utilized in suspension bath printing. [12,[19][20][21][22][23]27,[30][31][32] Agarose based granular suspension baths, consisting of agarose particles (i.e., irregular, spikey morphology of diameter ≈ 50 μm) suspended in an aqueous fluid, are also popular in suspension bath printing due to their low-cost, ease of use, and rapid recovery of viscosity during printing. [6,33,34] Gelatin methacrylamide (GelMA) was selected as a model non-Newtownian ink for assessment with each suspension bath, due to its commercial availability, cytocompatibility, cell degradability, and wide use in bioprinting.…”

Section: Introductionmentioning

confidence: 99%

“…[7][8][9][10][11] This method expands the potential for 3D bioprinting, but it also adds additional parameters to an already complex process, further complicating print parameter characterization and optimization. [2,[8][9][10][11][12][13] While many predictive models are available for extrusion printing, few models have been previously reported for suspension bath printing. [14][15][16][17][18] Most published approaches have relied on laborious and inefficient guess-and-check methods to determine optimal printing parameters, limiting overall progress and translation across research groups.…”

Section: Introductionmentioning

confidence: 99%

“…[ 7–11 ] This method expands the potential for 3D bioprinting, but it also adds additional parameters to an already complex process, further complicating print parameter characterization and optimization. [ 2,8–13 ]…”

Section: Introductionmentioning

confidence: 99%

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Computational Modeling and Experimental Characterization of Extrusion Printing into Suspension Baths

Prendergast

Burdick

2021

Adv Healthcare Materials

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The extrusion printing of inks into suspension baths is an exciting tool, as it allows the printing of diverse and soft hydrogel inks into 3D space without the need for layer-by-layer fabrication. However, this printing process is complex and there have been limited studies to experimentally and computationally characterize the process. In this work, hydrogel inks (i.e., gelatin methacrylamide (GelMA)), suspension baths (i.e., agarose, Carbopol), and the printing process are examined via rheological, computational, and experimental analyses. Rheological data on various hydrogel inks and suspension baths is utilized to develop computational printing simulations based on Carreau constitutive viscosity models of the printing of inks within suspension baths. These results are then compared to experimental outcomes using custom print designs where features such as needle translation speed, defined in this work as print speed, are varied and printed filament resolution is quantified. Results are then used to identify print parameters for the printing of a GelMA ink into a unique guest-host hyaluronic acid suspension bath. This work emphasizes the importance of key rheological properties and print parameters for suspension bath printing and provides a computational model and experimental tools that can be used to inform the selection of print settings.

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Section: Impact Of Print Speed On Printed Filamentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Computational Modeling and Experimental Characterization of Extrusion Printing into Suspension Baths

Prendergast

Burdick

2021

Adv Healthcare Materials

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“…Zhang et al recently utilized jammed HMPs made from Carbomer 980 as a supporting bath and 3D printed an ECM solution made from bovine Type I collagen into the void spaces between the HMPs in order to support the growth of cells. The jammed HMP material could be used for bioprinting of collagen 3D structures with minimal intermixing of the collagen and HMP materials, thus allowing for potential use in TE [ 116 ]. In a different approach, bioprinted granular hydrogels were shown to be able to support chondrogenic differentiation of hMSCs.…”

Section: Microspheres Forming Granular Hydrogels As 3d Tissue Engineering (Te) Constructsmentioning

confidence: 99%

A Review of the Use of Microparticles for Cartilage Tissue Engineering

Kulchar

Denzer

Chavre

et al. 2021

IJMS

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Tissue and organ failure has induced immense economic and healthcare concerns across the world. Tissue engineering is an interdisciplinary biomedical approach which aims to address the issues intrinsic to organ donation by providing an alternative strategy to tissue and organ transplantation. This review is specifically focused on cartilage tissue. Cartilage defects cannot readily regenerate, and thus research into tissue engineering approaches is relevant as a potential treatment option. Cells, scaffolds, and growth factors are three components that can be utilized to regenerate new tissue, and in particular recent advances in microparticle technology have excellent potential to revolutionize cartilage tissue regeneration. First, microspheres can be used for drug delivery by injecting them into the cartilage tissue or joint space to reduce pain and stimulate regeneration. They can also be used as controlled release systems within tissue engineering constructs. Additionally, microcarriers can act as a surface for stem cells or chondrocytes to adhere to and expand, generating large amounts of cells, which are necessary for clinically relevant cell therapies. Finally, a newer application of microparticles is to form them together into granular hydrogels to act as scaffolds for tissue engineering or to use in bioprinting. Tissue engineering has the potential to revolutionize the space of cartilage regeneration, but additional research is needed to allow for clinical translation. Microparticles are a key enabling technology in this regard.

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