Driven by the increasing interest in nanotechnology, materials that spontaneously form ordered nanostructures are becoming an important area of investigation. The development of new polymer-based biomaterials relies on knowledge of the underlying relations between the properties and the structure of these materials at multiple length scales. On the basis of sources, biomaterial polymer films are categorized into two different classes. In the first class, polymers are made from protein, natural rubber, cellulose, etc. these are also called natural polymers. The second category is largest one; it carries the artificial polymer or synthesized polymer, where natural polymers are chemically treated or synthesized in lab. Among these, chitosan is widely used linear polysaccharide biopolymer derived from chitin by deacetylation, chitosan has numerous advantages as a biomaterial; Chitosan is a unique biopolymer in the respect that it is abundant, cationic, lowtoxic, non-immunogenic, biodegradable, and due to its positive charges at