3D printing of Haversian bone–mimicking scaffolds for multicellular delivery in bone regeneration

Zhang, Meng; Lin, Rongcai; Wang, Xin; Xue, Jie; Deng, Chengbin; Feng, Chun; Zhuang, Hui; Ma, Jingge; Chen, Qin; Wan, Li; Chang, Jiang; Wu, Chengtie

doi:10.1126/sciadv.aaz6725

Cited by 263 publications

(221 citation statements)

References 40 publications

Supporting

Mentioning

215

Contrasting

Unclassified

Order By: Relevance

“…Typically, 3D-printed scaffolds are fabricated using fused deposition modeling (FDM) 3D printing methods. Recently, digital light processing (DLP) 3D printing has been used to produce bone scaffolds from a mixture of osteoceramic powder and polymeric binder [22,23]. Most previous studies did not attempt to completely remove the polymer binder due to concerns regarding the mechanical stability of the scaffold.…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Ceramic Bone Scaffolds with Variable Pore Architectures

Lim

Hong

Byeon

et al. 2020

IJMS

View full text Add to dashboard Cite

This study evaluated the mechanical properties and bone regeneration ability of 3D-printed pure hydroxyapatite (HA)/tricalcium phosphate (TCP) pure ceramic scaffolds with variable pore architectures. A digital light processing (DLP) 3D printer was used to construct block-type scaffolds containing only HA and TCP after the polymer binder was completely removed by heat treatment. The compressive strength and porosity of the blocks with various structures were measured; scaffolds with different pore sizes were implanted in rabbit calvarial models. The animals were observed for eight weeks, and six animals were euthanized in the fourth and eighth weeks. Then, the specimens were evaluated using radiological and histological analyses. Larger scaffold pore sizes resulted in enhanced bone formation after four weeks (p < 0.05). However, in the eighth week, a correlation between pore size and bone formation was not observed (p > 0.05). The findings showed that various pore architectures of HA/TCP scaffolds can be achieved using DLP 3D printing, which can be a valuable tool for optimizing bone-scaffold properties for specific clinical treatments. As the pore size only influenced bone regeneration in the initial stage, further studies are required for pore-size optimization to balance the initial bone regeneration and mechanical strength of the scaffold.

show abstract

Section: Introductionmentioning

confidence: 99%

3D-Printed Ceramic Bone Scaffolds with Variable Pore Architectures

Lim

Hong

Byeon

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Bone bioactive materials（BBMs）are supposed to be able to promote osteogenesis by inducing MSCs to differentiate into osteoblasts [ 20 , 21 ]. Thus, both in vitro osteogenic differentiation of MSCs [ [22] , [23] , [24] , [25] ] and in vivo osteogenesis are necessary to evaluate the performance of BBMs [ [26] , [27] , [28] , [29] , [30] , [31] ]. The research paradigm in detecting the fate of MSCs regulated by BBMs is evolving from low-throughput experimental design to high-throughput experimental design.…”

Section: Introductionmentioning

confidence: 99%

Development of methods for detecting the fate of mesenchymal stem cells regulated by bone bioactive materials

Jiang

Liu

Wang

et al. 2021

Bioactive Materials

View full text Add to dashboard Cite

The fate of mesenchymal stem cells (MSCs) is regulated by biological, physical and chemical signals. Developments in biotechnology and materials science promoted the occurrence of bioactive materials which can provide physical and chemical signals for MSCs to regulate their fate. In order to design and synthesize materials that can precisely regulate the fate of MSCs, the relationship between the properties of materials and the fate of mesenchymal stem cells need to be clarified, in which the detection of the fate of mesenchymal stem cells plays an important role. In the past 30 years, a series of detection technologies have been developed to detect the fate of MSCs regulated by bioactive materials, among which high-throughput technology has shown great advantages due to its ability to detect large amounts of data at one time. In this review, the latest research progresses of detecting the fate of MSCs regulated by bone bioactive materials (BBMs) are systematically reviewed from traditional technology to high-throughput technology which is emphasized especially. Moreover, current problems and the future development direction of detection technologies of the MSCs fate regulated by BBMs are prospected. The aim of this review is to provide a detection technical framework for researchers to establish the relationship between the properties of BMMs and the fate of MSCs, so as to help researchers to design and synthesize BBMs better which can precisely regulate the fate of MSCs.

show abstract

“…[310] Likewise, Haversian systemmimicking 3D scaffolds induced osteogenic, angiogenic and neurogenic differentiation in vitro and accelerated the ingrowth of blood vessels and new bone formation in vivo ( Figure 9B). [311] Similar macroscale architectural designs have been used for bone regeneration and nerve regeneration. Honeycomb β-tricalcium phosphate scaffolds with channel-like macropores and unidirectional interconnections demonstrated excellent osteogenesis in vivo.…”

Section: Architectural Challengesmentioning

confidence: 99%

“…[ 310 ] Likewise, Haversian system‐mimicking 3D scaffolds induced osteogenic, angiogenic and neurogenic differentiation in vitro and accelerated the ingrowth of blood vessels and new bone formation in vivo (Figure 9B). [ 311 ]…”

Section: A Materials System For Simultaneous Bone and Nerve Repairmentioning

confidence: 99%

Simultaneous Regeneration of Bone and Nerves Through Materials and Architectural Design: Are We There Yet?

Wan

Qin

Shen

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

Bilateral communication between bone and nerves is crucial for optimal repair of large bone defects as well as repair of peripheral nerves within the skeleton. However, simultaneous regeneration of bone and nerves is an issue that has long been overlooked in prior literature. Incongruous or even contradictory requirements or regeneration environments for bone and nerves make simultaneous regeneration of multiple tissues challenging. The present review commences with introducing natural crosstalk between bone and nerves, highlighting the rationale for simultaneous regeneration of bone and nerves. These issues will pave the way for the development of inspirational materials design concepts that capitalize on the principles of osteoconduction, osteoinduction, neuroconduction, neuroinduction, and neuroattraction. Potential strategies based on selection of appropriate scaffolds, acellular biological factors and architectural design will be addressed.

show abstract

3D printing of Haversian bone–mimicking scaffolds for multicellular delivery in bone regeneration

Cited by 263 publications

References 40 publications

3D-Printed Ceramic Bone Scaffolds with Variable Pore Architectures

3D-Printed Ceramic Bone Scaffolds with Variable Pore Architectures

Development of methods for detecting the fate of mesenchymal stem cells regulated by bone bioactive materials

Simultaneous Regeneration of Bone and Nerves Through Materials and Architectural Design: Are We There Yet?

Contact Info

Product

Resources

About