3D printing of PLA/n-HA composite scaffolds with customized mechanical properties and biological functions for bone tissue engineering

Wang, Wenzhao; Zhang, Boqing; Li, Mingxin; Li, Jun; Zhang, Chengyun; Han, Yanlong; Wang, Li; Wang, Kefeng; Zhou, Changchun; Liu, Lei; Fan, Yujiang; Zhang, Xingdong

doi:10.1016/j.compositesb.2021.109192

Cited by 227 publications

(112 citation statements)

References 57 publications

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…Composite scaffolds of PLA and n-HA were used with different percentages to induce osteogenesis in a rabbit model. The result indicated that the PLA/15% n-HA composite scaffold could maintain biological activity as well as suitable mechanical properties in the defect of the rabbit model [40]. Furthermore, designing a prosthetic device solely out of polymeric materials may appear to be feasible (because of their low elastic modulus), but their weak strength renders them unsuitable.…”

Section: Compositesmentioning

confidence: 99%

Active Materials for 3D Printing in Small Animals: Current Modalities and Future Directions for Orthopedic Applications

Memarian

Pishavar

Zanotti

et al. 2022

IJMS

View full text Add to dashboard Cite

The successful clinical application of bone tissue engineering requires customized implants based on the receiver’s bone anatomy and defect characteristics. Three-dimensional (3D) printing in small animal orthopedics has recently emerged as a valuable approach in fabricating individualized implants for receiver-specific needs. In veterinary medicine, because of the wide range of dimensions and anatomical variances, receiver-specific diagnosis and therapy are even more critical. The ability to generate 3D anatomical models and customize orthopedic instruments, implants, and scaffolds are advantages of 3D printing in small animal orthopedics. Furthermore, this technology provides veterinary medicine with a powerful tool that improves performance, precision, and cost-effectiveness. Nonetheless, the individualized 3D-printed implants have benefited several complex orthopedic procedures in small animals, including joint replacement surgeries, critical size bone defects, tibial tuberosity advancement, patellar groove replacement, limb-sparing surgeries, and other complex orthopedic procedures. The main purpose of this review is to discuss the application of 3D printing in small animal orthopedics based on already published papers as well as the techniques and materials used to fabricate 3D-printed objects. Finally, the advantages, current limitations, and future directions of 3D printing in small animal orthopedics have been addressed.

show abstract

Section: Compositesmentioning

confidence: 99%

Active Materials for 3D Printing in Small Animals: Current Modalities and Future Directions for Orthopedic Applications

Memarian

Pishavar

Zanotti

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…BMSCs have a strong ability of regeneration and differentiation. Cell therapies using BMSCs are currently involved in more than 900 clinical trials ( Kirsch et al, 2019 ; Wang et al, 2021 ). The fate of BMSCs is influenced by the microenvironment provided by the injection of hydrogels after transplantation.…”

Section: Discussionmentioning

confidence: 99%

“…In the repair of segmental bone defect, the two ends of the defect are completely disconnected, and the bone marrow cavity is completely exposed, so the biocompatibility of the repair material is highly required. Therefore, in the previous study, the lacunar bone defect model was used in the in vivo experiment of the composite scaffolds, and the repair of segmental bone defects by composite scaffolds is prone to infection and osteonecrosis ( Wang et al, 2021 ; Zhang et al, 2021 ). In the segmental bone repair model in this study, the biocompatibility advantage of the GelMA was demonstrated without bone infection.…”

Section: Discussionmentioning

confidence: 99%

Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration

Wang

et al. 2021

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Large-segment bone defect caused by trauma or tumor is one of the most challenging problems in orthopedic clinics. Biomimetic materials for bone tissue engineering have developed dramatically in the past few decades. The organic combination of biomimetic materials and stem cells offers new strategies for tissue repair, and the fate of stem cells is closely related to their extracellular matrix (ECM) properties. In this study, a photocrosslinked biomimetic methacrylated gelatin (Bio-GelMA) hydrogel scaffold was prepared to simulate the physical structure and chemical composition of the natural bone extracellular matrix, providing a three-dimensional (3D) template and extracellular matrix microenvironment. Bone marrow mesenchymal stem cells (BMSCS) were encapsulated in Bio-GelMA scaffolds to examine the therapeutic effects of ECM-loaded cells in a 3D environment simulated for segmental bone defects. In vitro results showed that Bio-GelMA had good biocompatibility and sufficient mechanical properties (14.22kPa). A rat segmental bone defect model was constructed in vivo. The GelMA-BMSC suspension was added into the PDMS mold with the size of the bone defect and photocured as a scaffold. BMSC-loaded Bio-GelMA resulted in maximum and robust new bone formation compared with hydrogels alone and stem cell group. In conclusion, the bio-GelMA scaffold can be used as a cell carrier of BMSC to promote the repair of segmental bone defects and has great potential in future clinical applications.

show abstract

“…The use of additive manufacturing techniques, also known as 3D printing, for the fabrication of customised bone tissue engineering architectures has developed very rapidly [ 1 , 2 , 3 ]. These fabrication techniques allow high reproducibility and repeatability, and they can process a wide range of natural and synthetic polymeric and composite materials, allowing for the fabrication of interconnected porous structures [ 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Novel 3D Bioglass Scaffolds for Bone Tissue Regeneration

et al. 2022

View full text Add to dashboard Cite

The design of scaffolds with optimal biomechanical properties for load-bearing applications is an important topic of research. Most studies have addressed this problem by focusing on the material composition and not on the coupled effect between the material composition and the scaffold architecture. Polymer–bioglass scaffolds have been investigated due to the excellent bioactivity properties of bioglass, which release ions that activate osteogenesis. However, material preparation methods usually require the use of organic solvents that induce surface modifications on the bioglass particles, compromising the adhesion with the polymeric material thus compromising mechanical properties. In this paper, we used a simple melt blending approach to produce polycaprolactone/bioglass pellets to construct scaffolds with pore size gradient. The results show that the addition of bioglass particles improved the mechanical properties of the scaffolds and, due to the selected architecture, all scaffolds presented mechanical properties in the cortical bone region. Moreover, the addition of bioglass indicated a positive long-term effect on the biological performance of the scaffolds. The pore size gradient also induced a cell spreading gradient.

show abstract

3D printing of PLA/n-HA composite scaffolds with customized mechanical properties and biological functions for bone tissue engineering

Cited by 227 publications

References 57 publications

Active Materials for 3D Printing in Small Animals: Current Modalities and Future Directions for Orthopedic Applications

Active Materials for 3D Printing in Small Animals: Current Modalities and Future Directions for Orthopedic Applications

Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration

Novel 3D Bioglass Scaffolds for Bone Tissue Regeneration

Contact Info

Product

Resources

About