3D Structure and Processing Methods Direct the Biological Attributes of ECM-Based Cardiac Scaffolds

Efraim, Yael; Schoen, Beth; Zahran, Sharbel; Davidov, Tzila; Vasilyev, Gleb; Baruch, Limor; Zussman, Eyal; Machluf, Marcelle

doi:10.1038/s41598-019-41831-9

Cited by 40 publications

(37 citation statements)

References 65 publications

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“…23,24 Amide I (1654 cm −1 ), amide II (1548cm −1 ), and amide III (1238cm −1 )-referred to as the collagen fingerprint-and glycosaminoglycan (1048 cm −1 ) peaks were also observed. 25,26 TXA-dECM bio-inks had the largest peaks, and the intensities decreased in the order TXA-> SDC-> SDS-dECM bio-inks. Figure 5(b) and (c) show the DSC results for the crosslinked dECM bio-inks.…”

Section: Analysis Of Intermolecular Bondingmentioning

confidence: 96%

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

2021

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Decellularized extracellular matrix-based bio-inks (dECM bio-inks) for bioprinting technology have recently gained attention owing to their excellent ability to confer tissue-specific functions and 3D-printing capability. Although decellularization has led to a major advancement in bio-ink development, the effects of detergent type, the most important factor in decellularization, are still unclear. In this study, the effects of various detergent types on bio-ink performance were investigated. Porcine liver-derived dECM bio-inks prepared using widely used detergents, including sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), Triton X-100 (TX), and TX with ammonium hydroxide (TXA), were characterized in detail. SDS and SDC severely damaged glycosaminoglycan and elastin proteins, TX showed the lowest rate of decellularization, and TXA-based dECM bio-ink possessed the highest ECM content among all bio-inks. Differences in biochemical composition directly affected bio-ink performance, with TXA-dECM bio-ink showing the best performance with respect to gelation kinetics, intermolecular bonding, mechanical properties, and 2D/3D printability. More importantly, cytocompatibility tests using primary mouse hepatocytes also showed that the TXA-dECM bio-ink improved albumin secretion and cytochrome P450 activity by approximately 2.12- and 1.67-fold, respectively, compared with the observed values for other bio-inks. Our results indicate that the detergent type has a great influence on dECM damage and that the higher the dECM content, the better the performance of the bio-ink for 3D bioprinting.

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Section: Analysis Of Intermolecular Bondingmentioning

confidence: 96%

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

2021

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“…A scaffold containing the abundant ECM protein collagen maintained cardiomyocyte contractile function for a period of 17 days with high levels of desmin expression. [ 102 ] ECM decellularized from the porcine ventricular tissue has been reported to provide natural cardiac ECM compositionally [9d,103] . Besides ECM proteins, the use of other naturally derived proteins, such as keratin [ 104 ] and SF [ 105 ] that are easy to obtain, have likewise been investigated.…”

Section: Tissue Regeneration Based On 3d Electrospun Scaffoldsmentioning

confidence: 99%

3D Electrospun Synthetic Extracellular Matrix for Tissue Regeneration

Toftdal

Friec

et al. 2021

Small Science

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Tissue regeneration is a rapidly evolving and interdisciplinary field at the intersection of life science, biology, material science, and engineering. Centrally, it involves functional cell-free or cell-laden constructs or biomaterial scaffolds that are fabricated utilizing various strategies. [1] Cell-free biomaterial scaffolds implanted directly at the sites of injury may mechanically support local cells to promote local tissue repair. [2] Furthermore, they can be functionalized both on the surface or in the interior with bioactive materials to stimulate regeneration of functional tissues. [3] In cell therapy applications, cell-laden constructs may enhance both survival and differentiation of the therapeutic cells compared with cell transplantation alone. The grafted cells may then ideally replace lost tissue and/or exert beneficial effects on the host tissue. [4] Among different biomaterial constructs, 3D fibrous scaffolds recreate a 3D microenvironment, which closely resembles the native extracellular matrix (ECM), including both structural and biochemical properties that guide cell survival and differentiation. [5] Scaffolds with fibrous networks possess unique characteristics, including sufficiently high interconnected porosity, high specific surface area, tunable mechanical properties, as well as optimal morphological features. The high porosity of the fibrous scaffolds facilitates mass transfer for effective nutrient supply, oxygen diffusion, metabolic waste removal, and enhancement of intercellular communications, consequently allowing high cell viability and function throughout the entire scaffold. [6] In addition, compared with 2D culture, the 3D cell culture provides a more realistic biochemical and biomechanical microenvironment, [7] creating an optimal environment for cell migration, proliferation, and differentiation. Hence, nanofibrous scaffolds with appropriate biomechanical properties are highly suitable for tissue engineering. [8] Electrospinning, as a relatively simple and versatile fiber preparation technique, has been developed to create fiber-based constructs in combination with cells, bioactive molecules, proteins, and biocompatible nanomaterials. [9] Many studies have demonstrated that the electrospinning technology has the potential for significant progress within the field of tissue regeneration. Chen et al. [10] summarized the methods for producing electrospun 1D nanofiber bundles, 2D

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“…The dECM consists of a variety of biologically active proteins and natural bioactive polymers of peptidoglycans and glycoproteins, 61‐64 which can be used as bioinks. dECM scaffolds mimic tissue‐specific components in 3D printed tissue products 65,66 . The use of decellularized scaffolds for cardiac tissue engineering application has been progressed in the past decade 22,67,68 .…”

Section: Bioinksmentioning

confidence: 99%

Progress in cardiovascular bioprinting

Quadri

Soman²,

Vijayavenkataraman

2021

Artificial Organs

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Cardiovascular disease has been the leading cause of death globally for the past 15 years. Following a major cardiac disease episode, the ideal treatment would be the replacement of the damaged tissue, due to the limited regenerative capacity of cardiac tissues. However, we suffer from a chronic organ donor shortage which causes approximately 20 people to die each day waiting to receive an organ. Bioprinting of tissues and organs can potentially alleviate this burden by fabricating low cost tissue and organ replacements for cardiac patients. Clinical adoption of bioprinting in cardiovascular medicine is currently limited by the lack of systematic demonstration of its effectiveness, high costs, and the complexity of the workflow. Here, we give a concise review of progress in cardiovascular bioprinting and its components. We further discuss the challenges and future prospects of cardiovascular bioprinting in clinical applications.

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3D Structure and Processing Methods Direct the Biological Attributes of ECM-Based Cardiac Scaffolds

Cited by 40 publications

References 65 publications

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

3D Electrospun Synthetic Extracellular Matrix for Tissue Regeneration

Progress in cardiovascular bioprinting

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