The article is devoted to substantiating use effectiveness of Anti Gravity fitness exercises in PE practice for relatively healthy female students. The studies were attended by students (n=25) of age 19 years practicing fitness aerobics in PE classes at university. The study period was being conducted during 10 months. Moreover, girls from group №1 (n=13) continued fitness aerobics classes. Besides, girls from group №2 (n=12) practiced Anti Gravity fitness together with fitness aerobics classes. The battery tests were used assessing physical fitness indicators: BMI, dynamometry, step ergometry, Flamingo balance test, Unterberg test, Hirtz test and others. By the way, statistical reliability was calculated using the Mann-Whitney U-test. The results had revealed the presence of significant (P<0,05) superiority particulars of indices of the static body balance and muscle strength of the hands in favor of female students practicing Anti Gravity fitness. Also, significant (P<0,05) superiority of these students was found in terms of characterizing the development of body flexibility. However, complex use of Anti Gravity fitness had been substantiated together with other types of health and sports fitness in РЕ practice of female students without diseases.