3β-Hydroxy-Δ5-steroidal congeners from a column fraction of Dendronephthya puetteri attenuate LPS-induced inflammatory responses in RAW 264.7 macrophages and zebrafish embryo model

Abstract

Bioactive compounds from marine organisms and their action mechanisms have provided new insights into medicinal and natural product research. Here, we report the identification of 3b-hydroxy-D5-steroidal congeners from a purified column fraction (DPCMH24) of the soft coral Dendronephthya puetteri harvested from Jeju, South Korea. DPCMH24 exerted strong anti-inflammatory effects through a dosedependent decrease in the levels of nitric oxide (NO) in LPS-induced RAW 264.7 macrophages (IC 50 value ¼ 6.54 AE 0.38 mg mL À1 ). Further, DPCMH24 attenuated the levels of PGE 2 and the proinflammatory cytokines, TNF-a, IL-1b, and IL-6. The above effects were mediated via the inhibition of nuclear factor kB activation and mitogen-activated protein kinase pathways. In vivo evaluation indicated that DPCMH24 reduced NO, iNOS, COX-2, ROS production and cell death in LPS-induced zebrafish embryos, confirming its anti-inflammatory potential. The constituent compounds were identified by GC-MS/MS analysis. These findings suggest that the steroidal congeners from D. puetteri may offer ample therapeutic potential against LPS-induced inflammation.

Particulate Matter-Induced Inflammation/Oxidative Stress in Macrophages: Fucosterol from Padina boryana as a Potent Protector, Activated via NF-κB/MAPK Pathways and Nrf2/HO-1 Involvement

“…The scan range was 50–500 m/z. Helium was used as the carrier gas with a constant flow rate of 0.73 mL/min [ 55 ].…”

Antioxidant Potential of Sulfated Polysaccharides from Padina boryana; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

“…The bands were developed and photographed via a FUSION SOLO Vilber Lourmat system. ImageJ program was assisted in the quantification of the band intensities [55,56].…”

Natural Products from Octocorals of the Genus Dendronephthya (Family Nephtheidae)

“…Moreover, this congener reduced the level of PGE 2 TNF-α, IL-1β, and IL-6. The anti-inflammatory effects of this sterol-rich mixture was confirmed in an LPS-stimulated in vivo zebrafish model by the downregulation of NO, iNOS, COX-2, ROS production and cell death [85,86], and this sterol rich congener showed cytotoxicity toward HL-60 and MCF-7 cells with IC 50 values of 25.27 and 22.81 µg/mL, respectively [87]. tory effects of this sterol-rich mixture was confirmed in an LPS-stimulated in vivo zebrafish model by the downregulation of NO, iNOS, COX-2, ROS production and cell death [85,86], and this sterol rich congener showed cytotoxicity toward HL-60 and MCF-7 cells with IC50 values of 25.27 and 22.81 µ g/mL, respectively [87].…”

“…It is interesting to note that compound 94 (capnell-9-ene-8β,10α-diol) inhibited the interaction of oncogenic transcription factor Myc (a family of regulator genes and proto-oncogenes that code for transcription factors) with its partner protein Max (inhibition = 77%) in yeast [91]. tory effects of this sterol-rich mixture was confirmed in an LPS-stimulated in vivo zebrafish model by the downregulation of NO, iNOS, COX-2, ROS production and cell death [85,86], and this sterol rich congener showed cytotoxicity toward HL-60 and MCF-7 cells with IC50 values of 25.27 and 22.81 µ g/mL, respectively [87].…”

“…In 2018, Jeon’s group reported the isolation of a 3β-hydroxy-Δ 5 -steroidal congener, consisting of six sterols, cholesterol ( 26 ) [ 30 ] ( Figure 4 ), cholesta-5,22-dien-3β-ol ( 34 ) [ 40 ], ergosta-5,22-dien-3β-ol ( 36 ) [ 41 ], stigmasta-5-en-3β-ol ( 39 ) [ 48 ], 22,23-methylenecholesterol ( 40 ) [ 49 ] ( Figure 7 ), and cholesta-5,24-dien-3β-ol ( 88 ) [ 84 ] ( Figure 14 ), from D. puetteri , collected from the Jeju Island, South Korea [ 85 ]. The structures for all sterols 26 , 34 , 36 , 39 , 40 , and 88 were determined by GC-MS/MS analysis [ 85 ]. In lipopolysaccharides (LPS)-stimulated RAW264.7 cells, this mixture inhibited nitric oxide (NO) production with an IC 50 value of 6.54 µg/mL.…”