4. Kestose: a trisaccharide formed from sucrose by yeast invertase

Albon, N.; Bell, David; Blanchard, P. H.; Gross, D.; Rundell, J. T.

doi:10.1039/jr9530000024

Cited by 63 publications

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“…The analysis of these fructooligosaccharides indicated that they were composed of 6-kestose (61.5%), neokestose (29.7%), and 1-kestose (8.8%). It was reported in the literature that the production of these fructooligosaccharides also occurred during the hydrolysis of sucrose by invertase (24)(25)(26)(27)(28)(29)(30). This enzyme was responsible for fructosyl transfer to the primary hydroxyl groups of sucrose.…”

Section: Resultsmentioning

confidence: 96%

Production of Fructose and Ethanol from Sugar Beet Molasses Using Saccharomyces cerevisiae ATCC 36858

Atiyeh

2002

Biotechnology Progress

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The production of enriched fructose syrups and ethanol from beet molasses using Saccharomyces cerevisiae ATCC 36858 was studied. In batch experiments with a total sugar concentration between 94.9 and 312.4 g/L, the fructose yield was above 93% of the theoretical value. The ethanol yield and volumetric productivity in the beet molasses media with sugar concentration below 276.2 g/L were in the range of 59-76% of theoretical value and between 0.48 and 2.97 g of ethanol/(L x h), respectively. The fructose fraction in the carbohydrates content of the produced syrups was more than 95% when the total initial sugar concentration in the medium was below 242.0 g/L. Some oligosaccharides and glycerol were also produced in all tested media. Raffinose and the produced oligosaccharides were completely consumed by the end of the fermentation process when the total initial sugar concentration was below 190.1 g/L. The glycerol concentration was below 16.1 g/L. The results could be useful for a potential industrial production of ethanol and high-fructose syrup from sugar beet molasses.

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Section: Resultsmentioning

confidence: 96%

Production of Fructose and Ethanol from Sugar Beet Molasses Using Saccharomyces cerevisiae ATCC 36858

Atiyeh

2002

Biotechnology Progress

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“…from grasses (Bacon & Edelman, 1950; Albon et al Whilst a range of evidence has been presented 1953; Bacon & Bell, 1953;Gross, Blanchard & Bell, which supports the view that sucrose is the precursor 1954; Allen & Bacon, 1956;Edeiman, 1956; Gross, of fructan in grass tissue (summarized in Cairns, 1962;Schaffler & Morel du Boil, 1972;Straathof, 1989), the enzymology of the process and the Kieboom & van Bekkum, 1986;Pollock c/a/., 1989). physiological significance of SST retnains incom-It follows that the measured properties of SST from pletely understood (Cairns, Winters & Pollock, mixed enzyme reactions containing both of these activities are untrustworthv and difficult to interpret.…”

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confidence: 99%

The interpretation of in vitro measurements of fructosyl transferase activity: an analysis of patterns of fructosyl transfer by fungal invertase

Cairns

Ashton

1991

New Phytologist

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SUMMARYA commercially available invertase preparation from yeast was analysed by native gel electrophoresis and shown to contain the large and small forms of yeast invertase but no specific fructosyl transferase. Both invertase isoforms catalysed the synthesis of the trisaccharides isokestose, neokestose and kestose from 600 mol m"^ sucrose at pH 5.The hydrolytic and fructosyl transferase properties of the unfractionated invertase preparation were analysed under conditions similar to those used for the analysis of sucrose : sucrose fructosyl transferase (SST) from grasses. The sucrose concentration kinetics of the transferase gave a K^ of between 224-308 mol m"^ which is similar to that reported for SST from graminaceous and other plant species. The sucrose K^ of the hydrolase was estimated at between 11-34 mol m"^.The transferase and hydrolase activities of yeast invertase could be distinguished on the basis of their responses to reaction temperature and pH, and in these respects, the data reproduced the results of grass SST studies. These observed differences in properties can be explained in terms of changes in reaction sucrose concentration and the differential kinetic properties of the hydrolase and transferase activities.The two activities could not be distinguished on the basis of their temperature-stability characteristics or their responses to pyridoxal HCl. Pyridoxal HCl was shown to cause acidification of enzyme reactions and the direct effects of this compound on the transferase and hydrolase activities were thus difficult to interpret.The results are discussed with respect to the evidence for separate SST and invertase activities in mixed enzyme systems derived from grass tissues.

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“…were detected on the paper in the presence of borate either with urea-phosphoric acid (Wise et al 1955) or x-naphtholphosphoric acid (Albon, Bell, Blanchard, Gross & Rundell, 1953). lonophoretic mobilities in a high-voltage field (Gross, 1953) were kindly determined by Dr D. Gross. Modified diazouracil test. The diazouracil test, originally designed by Raybin (1933) for use with 40-50 mg. of sugar, was adapted for use with 0 5 mg. of sucrose or raffinose.…”

Section: Methodsmentioning

confidence: 99%

The mechanism of polysaccharide production from sucrose. 4. Isolation and probable structures of oligosaccharides formed from sucrose by a levansucrase system

Feingold

Avigad

Hestrin

1956

Biochemical Journal

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4. Kestose: a trisaccharide formed from sucrose by yeast invertase

Cited by 63 publications

References 0 publications

Production of Fructose and Ethanol from Sugar Beet Molasses Using Saccharomyces cerevisiae ATCC 36858

Production of Fructose and Ethanol from Sugar Beet Molasses Using Saccharomyces cerevisiae ATCC 36858

The interpretation of in vitro measurements of fructosyl transferase activity: an analysis of patterns of fructosyl transfer by fungal invertase

The mechanism of polysaccharide production from sucrose. 4. Isolation and probable structures of oligosaccharides formed from sucrose by a levansucrase system

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