[40] Interactions of retinoids with phospholipid membranes: Optical spectroscopy

Stillwell, William; Wassall, Stephen R.

doi:10.1016/0076-6879(90)89311-5

Cited by 17 publications

(13 citation statements)

References 17 publications

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“…In the current study, we investigated membrane fluidity at 29 and 55°C, which monitor the gel and liquid crystalline phase of the membrane, respectively as seen from the DSC studies. To achieve this, the bandwidth of CH 2 antisymmetric stretching vibrational modes of DPPC liposomes (Toyran and Severcan 2003;Severcan et al 2005) and absorbance values at 440 nm (Severcan et al 2000(Severcan et al , 1995Chong and Colbow 1976;Stillwell and Wassall 1990;Viti et al 1985 ;Ohki and Duzgunes 1979) were studied. All experiments were repeated five times and similar trends were observed at each repeat.…”

Section: Resultsmentioning

confidence: 99%

Concentration Dependent Different Action of Tamoxifen on Membrane Fluidity

Kazancı

Severcan

2007

Bioscience Reports

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Tamoxifen (TAM) is a non-steroidal antiestrogen drug, which is widely used to prevent and treat breast, liver, pancreas and brain cancers. The present work investigates, in detail, the concentration dependent behavior of TAM (varying from 1 mol% to 45 mol%) on membrane fluidity. The differential scanning calorimetry (DSC) studies showed that tamoxifen eliminates the pre-transition and decreases the main phase transition to lower temperatures. Using visible spectroscopy at 440 nm and Fourier transform infrared (FTIR) spectroscopy it was found that membrane dynamics decreases for 1 and 3 mol% tamoxifen in both the gel and liquid crystalline phases. Above these concentrations up to 18-24 mol%, it increases and reaches its maximum values. As tamoxifen concentration was further increased, the membrane dynamics is found to be gradually decreased, although TAM still has fluidifying effect in comparison to pure phospholipid membrane. These findings are important for the effective use of tamoxifen in the cancer therapy to eliminate its dose dependent side effects reported in the literature.

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Section: Resultsmentioning

confidence: 99%

Concentration Dependent Different Action of Tamoxifen on Membrane Fluidity

Kazancı

Severcan

2007

Bioscience Reports

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“…6 and 7). It is considered that this protein is a carrier of retinoid such as retinoic acid and retinol [27] which affect the permeability of the plasma membrane while interacting with phospholipids, resulting in greater input of ions from the outside [28]. This phenomenon may be related to cryocapacitation due to access of ions such as Ca 2+ which turns out to be acrosome and hypermotility reaction [14].…”

Section: Resultsmentioning

confidence: 99%

“…While combinations with spermatozoa from the high freezability males showed lower HSP90a reduction and no change in the concentration of L-PGDS in the same period, which can ensure greater protection to thermal and oxidative stress by HSP90a [5] and smaller amounts of retinoic acid and retinol that promote changes in the membrane permeability [28]. In addition, the seminal plasma of males of high freezability had greater concentration of the NPC2 protein of 19 kDa which was reduced by incubating with high freezability spermatozoa, which in turn could be associated with a better prevention mechanism of cryocapacitation of these sperm by effect of plasma [38].…”

Section: Resultsmentioning

confidence: 99%

“…These two latter molecules are involved in the early capacitation [14], because they change the permeability of the plasma membrane [28]. Additionally, L-PGDS is present in the acrosomal membrane of freshly ejaculated spermatozoa and then disappears within the sperm acrosome reaction [25], and the in vitro incubation of sperm with this protein increases their binding capacity to the zona pellucida [29].…”

Section: Introductionmentioning

confidence: 99%

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Relationship between HSP90a, NPC2 and L-PGDS proteins to boar semen freezability

Valencia

Gómez

López

et al. 2017

J Animal Sci Biotechnol

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BackgroundThe purpose of this study was to determine the association of three proteins involved in sperm function on the freezability of porcine semen: the heat shock protein 90 alpha (HSP90a), the Niemann-Pick disease type C2 protein (NPC2), and lipocalin-type prostaglandin D synthase (L-PGDS).Six adult boars (each boar was ejaculated three times, 18 in total) were classified by freezability based on the percentage of functionally competent sperm. The male semen with highest freezability (MHF) and the male semen with lowest freezability (MLF) were centrifuged immediately after collection to separate seminal plasma and spermatozoa to make four possible combinations of these two components and to incubate them for 3 h, adjusting the temperature to 17 °C, to freeze them afterwards. The quantification of proteins was performed in two stages: at zero and at 3 h after incubation of the four combinations.ResultsThe spermatozoa × incubation time (IT) interaction only had effect (P < 0.01) on HSP90a levels; this protein increased in seminal plasma, after 3 h of incubation, in larger quantity (P < 0.05) in combinations with MLF spermatozoa. In relation with the NPC2 protein, two isoforms of 16 and 19 kDa were identified. The 19 kDa isoform was affected (P < 0.01) only by the seminal plasma × IT interaction, with superior values (P < 0.01) both at zero and three hours of incubation, in the combinations with MHF seminal plasma; and 16 kDa isoform was affected (P < 0.01) only by the IT with reduction after 3 h of incubation. The levels of L-PGDS was affected (P < 0.01) only by the spermatozoa × IT interaction, which reduced (P < 0.01) in combinations with MLF spermatozoa after 3 h of incubation.ConclusionsIt is possible to consider that the three proteins evaluated were associated with freezability of boar semen due, especially, to the fact that mixtures with MLF spermatozoa showed greater increase levels of the HSP90a protein and reduction of L-PGDS in plasma. In addition, the seminal plasma of MHF had higher concentration of the NPC2 of 19 kDa protein, which was reduced by incubating with MHF spermatozoa.

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“…Major plant sterols all satisfy these conditions and are accordingly able to stabilize lipid bilayer (85), to order phospholipid acyl chain, and consequently to reduce membrane water per-FIGURE 6. Free sterols promote various spatial organization of membrane.…”

Section: Free and Conjugated Phytosterols Exhibit Various Abilities Tmentioning

confidence: 99%

Differential Effect of Plant Lipids on Membrane Organization

Grosjean

Mongrand

Beney

et al. 2015

Journal of Biological Chemistry

105

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Background: The effect of the wide variety of plant lipids on membrane organization is still poorly understood. Results: The local amounts of phytosphingolipids and free and conjugated phytosterols control the relative proportion and spatial distribution of ordered domains. Conclusion: Plant lipids differently modulate, alone or in combination, membrane heterogeneity. Significance: Our results highlight how plant lipid diversity might drive the plasma membrane subcompartmentalization.

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[40] Interactions of retinoids with phospholipid membranes: Optical spectroscopy

Cited by 17 publications

References 17 publications

Concentration Dependent Different Action of Tamoxifen on Membrane Fluidity

Concentration Dependent Different Action of Tamoxifen on Membrane Fluidity

Relationship between HSP90a, NPC2 and L-PGDS proteins to boar semen freezability

Differential Effect of Plant Lipids on Membrane Organization

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