[42] Purification of calf liver tRNA's

Pearson, R.L.; Weiss, Joseph F.; Holladay, D.W.; Kelmers, A.D.

doi:10.1016/0076-6879(74)29047-5

Cited by 6 publications

(4 citation statements)

References 7 publications

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“…tRNA"' was purified by consecutive column chromatography on BD-cellulose (benzoylated DEAE-cellulose) and DEAE-Sephadex, as described for tRNA"' (Kryukov et al, 1972), and then on hydroxyapatite by the method of Pearson & Kelmers (1966) and on RPC-5 system as described by Pearson et al (1970). The tRNA "'V finally obtained was about 90 % pure.…”

Section: Methodsmentioning

confidence: 99%

The separation of oligonucleotides of baker's-yeast valine transfer ribonucleic acid 2b by high-voltage electrophoresis on DEAE-paper and by thin-layer chromatography

Gorbulev¹,

Barciszewski²,

Axelrod³

1977

Biochemical Journal

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Section: Methodsmentioning

confidence: 99%

The separation of oligonucleotides of baker's-yeast valine transfer ribonucleic acid 2b by high-voltage electrophoresis on DEAE-paper and by thin-layer chromatography

Gorbulev¹,

Barciszewski²,

Axelrod³

1977

Biochemical Journal

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“…Although minimal additional purification was achieved on RPC-5 reverse phase columns (Pearson et al, 1971), FUra-substituted Val tRNA J was resolved into two varients, denoted (A) and B Aminoacyl-tRNA synthetases were purified to remove tRNA and nucleases by the procedure of Muench and Berg (1966) This fraction was then assayed for valine tRNA synthetase activity (Scott, 1968) Time points (0 to 60 min) were tested to determine the steady-state inter val for the reaction.…”

Section: Rpc-5mentioning

confidence: 99%

19F NMR studies of the solution structure and dynamics of 5-fluorouracil-substituted valine tRNA from Escherichia coli

Hardin

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“…The antibody used in this study was produced in rabbits against mouse heart MnSOD. The MnSOD was purified according to methods of Pearson et al (1980). The unpurified antisera was treated with ammonium sulfate to 50% saturation.…”

Section: Differentiation Experimentsmentioning

confidence: 99%

Superoxide dismutase activities of differentiating clones from an immortal cell line

Oberley

Ridnour

Sierra-Rivera

et al. 1989

Journal Cellular Physiology

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We have studied superoxide dismutase (SOD) levels in the X-REF-23 rat embryo fibroblast cell line. X-REF-23 is an immortal cell line that maintains a nontransformed phenotype throughout its known lifespan. Low-passage X-REF-23 cells undergo spontaneous differentiation into muscle and adipose cells, while high-passage X-REF-23 cells undergo little or no differentiation. SOD activities were measured in subclones of X-REF-23, which differentiate into muscle (AMC subclone) or adipose (AMB-J) cells, as well as the parental nondifferentiating X-REF-23 cells. Total SOD activity increased in all three cell lines with time in culture. Cu-ZnSOD was induced in the AMB-J and the X-REF-23 cells with time in culture, whereas the AMC cells showed no induction. MnSOD activity was induced during the time period in which differentiation occurred in the two differentiating clones. In contrast, MnSOD was not induced in this time period in the nondifferentiating X-REF-23 cell line. However, MnSOD activity was induced in the latter cell line at a much later time. Levels of immunoreactive MnSOD correlated quite well with MnSOD activity in all three cell lines. The nondifferentiating X-REF-23 cells, but not the two differentiating cells lines, showed a large increase in cell organelles with time in culture. In particular, an increase in very small mitochondria was observed; these mitochondria often showed evidence of disorganization.

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[42] Purification of calf liver tRNA's

Cited by 6 publications

References 7 publications

The separation of oligonucleotides of baker's-yeast valine transfer ribonucleic acid 2b by high-voltage electrophoresis on DEAE-paper and by thin-layer chromatography

The separation of oligonucleotides of baker's-yeast valine transfer ribonucleic acid 2b by high-voltage electrophoresis on DEAE-paper and by thin-layer chromatography

19F NMR studies of the solution structure and dynamics of 5-fluorouracil-substituted valine tRNA from Escherichia coli

Superoxide dismutase activities of differentiating clones from an immortal cell line

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