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Чехонин, В. П.; Gurina, O. I.; Dmitrieva, T. B.; Shepeleva, I. I.; Ryabukhin, I. A.; Babich, G N

doi:10.1023/a:1013090214269

Cited by 6 publications

(4 citation statements)

References 7 publications

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“…3, c). Since cells stained with the used antibodies morphologically resembled astrocytes, we performed double immunofl uorescent staining with monoclonal antibodies to BSAT1 and polyclonal antibodies to GFAP obtained previously [3]. We found that immunofl uorescence determined by antibodies to BSAT1 and GFAP is localized in the same cells (Fig.…”

Section: Resultsmentioning

confidence: 83%

Cloning and Expression of Brain-Specific Anion Transporter BSAT1 and Isolation of Monoclonal Antibodies to Its Extracellular Fragment

et al. 2012

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Brain-specific anion transporter BSAT1 extracellular fragment (451-557) cDNA was cloned in a vector for prokaryotic expression, a producer E. coli strain was obtained, and recombinant extracellular fragment BSAT1(451-557)was purified and used for immunization of BALB/c mice. Splenic cells from mice with verified immune response were used for hybridoma generation. Several hybridoma clones producing monoclonal antibodies to BSAT1 extracellular fragment were selected. Antibody specificity was confirmed by ELISA, immunoblotting with recombinant BSAT1(451-557), and immunofluorescent BSAT1 assay on rat brain sections and cultured HEK293 cells. It was demonstrated that the obtained antibodies specifically bind native rat and human BSAT1 and can be used in both fundamental studies of structures forming the blood-brain barrier and development of targeted transport of diagnostic preparations and drugs across the blood-brain barrier.

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Section: Resultsmentioning

confidence: 83%

Cloning and Expression of Brain-Specific Anion Transporter BSAT1 and Isolation of Monoclonal Antibodies to Its Extracellular Fragment

et al. 2012

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“…Monoclonal cells producing anti-GFAP antibodies were obtained by the standard method [8] with modifications [3]. PEG-DMSO was used as a coupling agent (Sigma).…”

Section: Methodsmentioning

confidence: 99%

“…Total protein content was measured in the reaction with bicinchoninic acid [3] using BSA Protein Assay Reagent Kit 23227 (Pierce). The sample (preparation of immunoliposomes, 0.1 ml) was mixed with 2 ml working solution and incubated at 37 o C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Polyethylene glycol-conjugated immunoliposomes specific for olfactory ensheathing glial cells

et al. 2008

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A novel immunoliposome delivery system was developed for directed transport into cultured olfactory epithelium cells. Monoclonal antibodies against glial fibrillary acidic protein (GFAP) served as a vector. Fluorescence microscopy showed that the target cells are specifically stained with Dil dye incorporated into liposomal membranes. This transport system holds promise for the delivery of bioactive substances to olfactory epithelial cells and modulation of their capacity to stimulate axonal regeneration.

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“…Hybridoma producing these antibodies was created as a result of immunization of mice with preparations of cerebral capillary fractions isolated using a previously described and modified method [6]. We obtained monoclonal antibodies to glial fibrillary acidic protein (GFAP), the main marker of fibrillar astrocytes [2].…”

mentioning

confidence: 99%

Combined immunoperoxidase analysis for visualization of cells of the blood-brain barrier

et al. 2006

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A method for double immunoperoxidase staining of the blood-brain barrier cell elements was developed using 2mB6 monoclonal antibodies specifically visualizing brain capillary antigen and antibodies to glial fibrillary acidic protein (GFAP). The method is based on consecutive visualization of antigen structures in one section: first using a substrate mixture containing diaminobenzidine (brown coloring) and then a mixture with diaminobenzidine and CoCl(2) (blue coloring). This method visualizes cerebral capillary cells and fibrillar astrocytes interacting with them. The method can be used in all variants of double immunoperoxidase studies and for immunodetection of several antigens in immunoblotting analysis.

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Cited by 6 publications

References 7 publications

Cloning and Expression of Brain-Specific Anion Transporter BSAT1 and Isolation of Monoclonal Antibodies to Its Extracellular Fragment

Cloning and Expression of Brain-Specific Anion Transporter BSAT1 and Isolation of Monoclonal Antibodies to Its Extracellular Fragment

Polyethylene glycol-conjugated immunoliposomes specific for olfactory ensheathing glial cells

Combined immunoperoxidase analysis for visualization of cells of the blood-brain barrier

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