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Niwa, Kyosuke; Aruga, Yusho

doi:10.1023/a:1022998823920

Cited by 44 publications

(1 citation statement)

References 13 publications

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“…ITS sequences are not available as classification markers for Porphyra subspecies or ecotypes (Niwa and Aruga 2003;Xie et al 2007b). It has been reported that the IGS region is the predominant marker in classification and identification, while the ITS sequence can be used for study in close genocline species (Baldwin and Markos, 1998;Li et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Sequence analysis of rDNA intergenic spacer (IGS) of Porphyra haitanensis

Shen

et al. 2009

J Appl Phycol

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The intergenic spacer region (IGS) has been used for the first time to analyze the genetic variability of Porphyra haitanensis from different areas. In order to determine that whether the IGS sequences could be used for classification and identification in intraspecies of Porphyra, the partial IGS sequences of cultivated strains of P. haitanensis (isolated from Putian-Fujian Province, Shantou-Guangdong Province and Ningbo-Zhejiang Province), were amplified, sequenced and analyzed. The sequence analysis indicated that the partial IGS sequences from the three stains were the external transcribed spacers (ETS) of 3′ end of the IGS gene. In the three stains, the length of IGS sequences ranged from 1,085 to 1,100 bp and the G + C content varied from 50.88% to 51.27%. There were 55 variable sites which occupied approximately 5% of the ETS sequences. Similarity analysis and multisequencing alignment of sequences indicated that the partial IGS sequences of the three stains of P. haitanensis had notable variabilities. Therefore, the IGS sequence could be used as the critical genetic marker in intraspecies of P. haitanensis. Furthermore, IGS sequence analysis will be a powerful tool for genetic diversity and classification in intraspecies of other Porphyra species.

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Section: Discussionmentioning

confidence: 99%