[43] Malondialdehyde determination as index of lipid Peroxidation

Draper, H. H.; Hadley, M.

doi:10.1016/0076-6879(90)86135-i

Cited by 3,621 publications

(1,824 citation statements)

References 22 publications

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“…The protein content of supernatant was determined by protein assay reagent (BioRad Laboratories, Hercules, CA, USA; 500-0.006). Lipid peroxidation was evaluated by the thiobarbituric acid reactive substances procedure (Draper and Hadley 1990). Briefly, 210 μl of the resulting supernatant were mixed with 420 μl of 28% trichloroacetic acid solutions in an Eppendorf tube.…”

Section: Methodsmentioning

confidence: 99%

“…These age-related molecules include senescence-associated β-galactosidase (SA-β-Gal) (Dimri et al 1995), lipofuscin granules (Nakano et al 1995), lipid peroxidation (Draper and Hadley 1990;Almeida et al 1998), and protein oxidation (Welis-Knecht et al 1993). SA-β-Gal is a eukaryotic hydrolase that is localized in the lysosome (Kurz et al 2000), and SA-β-Gal expression increases with age (Dimri et al 1995;Kishi et al 2003;Genade et al 2005;Hsu et al 2008;Hsieh and Hsu 2011).…”

Section: Introductionmentioning

confidence: 99%

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The use of honeybees reared in a thermostatic chamber for aging studies

Hsu

Chan

2011

AGE

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Honeybees (Apis mellifera) are an attractive model system for studying aging. However, the aging level of worker honeybees from the field hive is in dispute. To eliminate the influence of task performance and confirm the relationship between chronological age and aging, we reared newly emerged workers in a thermostat at 34°C throughout their lives. A survivorship curve was obtained, indicating that workers can be reared away from the field hive, and the only difference between these workers is age. To confirm that these workers can be used for aging studies, we assayed age-related molecules in the trophocytes and fat cells of young and old workers. Old workers expressed more senescence-associated β-galactosidase, lipofuscin granules, lipid peroxidation, and protein oxidation than young workers. Furthermore, cellular energy metabolism molecules were also assayed. Old workers exhibited less ATP concentration, β-oxidation, and microtubule-associated protein light chain 3 (LC3) than young workers. These results demonstrate that honeybees reared in a thermostatic chamber can be used for aging studies and cellular energy metabolism in the trophocytes and fat cells of workers changes with advancing age.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The use of honeybees reared in a thermostatic chamber for aging studies

Hsu

Chan

2011

AGE

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“…The level of thiobarbituric acid reactive substance, malonaldehyde (MDA) as an index of lipid peroxidation was evaluated in the testicular tissue and pituitary gland samples using the double heating method of Draper and Hadley [34] . The MDA concentrations in the testes and pituitary glands were calculated by the absorbance coefficient of MDA-TBA complex 1.56 暳 10 5 /cm and expressed in nmol/mg of protein.…”

Section: Evaluation Of the Effect Of Treatments On Testicular Tissuementioning

confidence: 99%

Alleviating effects of melatonin on oxidative changes in the testes and pituitary glands evoked by subacute chlorpyrifos administration in Wistar rats

Umosen

Ambali

Ayo

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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“…24 The principle of the method is the spectrophotometric measurement of the color generated by the reaction of thiobarbituric acid (TBA) with MDA. For this purpose 2.5 mL of 100 g/L trichloroacetic acid solution was added to 0.5 mL supernatant in each centrifuge tube and the tubes placed in a boiling water bath for 15 min.…”

Section: Malondialdehyde Assaymentioning

confidence: 99%

Pentoxifylline reduces biochemical markers of ischemia-reperfusion induced spinal cord injury in rabbits

et al. 2002

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Study design: Occlusion of the infrarenal abdominal aorta with administration of pentoxifylline was applied to adult rabbits, followed by removal of aortic clamp and reperfusion. Tissue levels of cytokines, lipid peroxides, and antioxidant enzymes were assayed and compared within groups. Objectives: To examine the e ect of pentoxifylline (PTX) on cytokine levels, lipid peroxidation, and antioxidant enzymes in a rabbit model of spinal cord ischemia-reperfusion injury induced by aortic occlusion. Setting: Isparta, Turkey. Methods: Rabbits were randomly allocated into four groups of sham laparotomy (SHAM), sham laparotomy with PTX administration (SHAM+PTX), aortic occlusion and reperfusion (AOR), aortic occlusion and reperfusion with PTX administration (AOR+PTX). An intravenous bolus of 50 mg/kg PTX was given just before aortic cross clamping. An atraumatic microvascular clamp was then placed on the abdominal aorta immediately distal to the left renal artery for 30 min. PTX was infused at a rate of 0.5 mg/kg/min during the aortic occlusion. Animals were subjected to 120 min of reperfusion after removal of the aortic clamp. All animals were sacri®ced at the end of reperfusion. The lumbosacral segments of spinal cords were quickly harvested and stored at 7788C for biochemical assays of IL-6, TNF-a, MDA, SOD, and CAT levels. Di erences among groups were analyzed by one-way analysis of variance followed by a post hoc Tukey's honestly signi®cant di erence test. Results: No di erences in mean levels of IL-6, TNF-a, MDA, SOD, and CAT were noted between SHAM and SHAM+PTX groups (P40.05). There was a signi®cant increase in all biochemical parameters in the AOR group (P50.05). Administration of PTX signi®cantly attenuated the levels of all biochemical parameters in the AOR+PTX group (P50.05). Conclusion: PTX pretreatment attenuated ischemia-reperfusion induced spinal cord injury in a rabbit model, in terms of biochemical parameters of ischemia and reperfusion.

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[43] Malondialdehyde determination as index of lipid Peroxidation

Cited by 3,621 publications

References 22 publications

The use of honeybees reared in a thermostatic chamber for aging studies

The use of honeybees reared in a thermostatic chamber for aging studies

Alleviating effects of melatonin on oxidative changes in the testes and pituitary glands evoked by subacute chlorpyrifos administration in Wistar rats

Pentoxifylline reduces biochemical markers of ischemia-reperfusion induced spinal cord injury in rabbits

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