446: Successful Subzero Non-Freezing Preservation of Rat Lungs with Supercooling Technology

Okamoto, Toshihiro; Nakamura, Takayuki; Zhang, J.; Aoyama, Akihiro; Satoda, Naoki; Takahashi, Ayuko; Zhao, Xiangdong; Chen, F.; Fujinaga, Takuji; Hamakawa, Hiroshi; Tanaka, Shōji; Sakai, Hiroaki; Wada, Hiromi; Bando, Toru

doi:10.1016/j.healun.2007.11.458

Cited by 3 publications

(5 citation statements)

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“…All the lungs were reperfused successfully in 80 min. Thus, consistent with previous reports [12][13][14][15][16][17], our isolated rat lung perfusion model remained stable throughout the observation period. Airway resistance Airway resistance in the control group increased and was significantly higher than that in the sham group (P < 0.001 at 80 min after reperfusion).…”

Section: Physiological Parameterssupporting

confidence: 91%

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Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model

Ohsumi¹,

Chen²,

Nakajima³

et al. 2012

Transplant International

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Summary Warm ischemia‐reperfusion injury related to donation after cardiac death donors is a crucial and inevitable issue. As surfactant function is known to deteriorate during warm ischemia, we hypothesized that surfactant inhalation during warm ischemia would mitigate warm ischemia‐reperfusion injury. We used an isolated rat lung perfusion model. The rats were divided into three groups: sham, control, and surfactant. In the control and surfactant groups, cardiac arrest was induced by ventricular fibrillation. Ventilation was restarted 110 min later; subsequently, the lungs were flushed, and heart and lung block was recovered. In the surfactant group, a natural bovine surfactant Surfacten® was inhaled for 3 min at the end of warm ischemia. Then, the lungs were reperfused for 80 min. Surfactant inhalation significantly improved graft functions, effectively increased lung tissue ATP levels, and significantly decreased mRNA levels of IL‐6 and IL‐6/IL‐10 ratio at the end of reperfusion. Histologically, lungs in the surfactant group showed fewer signs of interstitial edema and hemorrhage, and significantly less neutrophilic infiltration than those in the control group. Our results indicated that surfactant inhalation in the last phase of warm ischemia maintained lung tissue energy levels and prevented cytokine production, resulting in the alleviation of warm ischemia‐reperfusion injury.

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Section: Physiological Parameterssupporting

confidence: 91%

“…Isolated rat lung perfusion (Model 829; Hugo-Sachs Elektronik-Harvard Apparatus) was performed as previously reported [12][13][14][15][16][17]. Rats were anesthetized with an intraperitoneal injection of sodium pentobarbital (50 mg/kg), intubated after tracheotomy, and ventilated with ambient air at positive pressure.…”

Section: Isolated Rat Lung Perfusion Modelmentioning

confidence: 99%

Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model

Ohsumi¹,

Chen²,

Nakajima³

et al. 2012

Transplant International

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“…There have been several reported studies to date that are similar to the present one on organ preservation in a subzero environment with the application of magnetic fields or voltage (8,9). In the report of Okamoto et al (9), 3000 V was applied in a preservation of a rat lung in a j2-C environment, and the preservation state 60 min after perfusion was reported to be better than with conventional lung preservation at 4-C. In a report by Monzen et al (8), a heart and liver were preserved for 24 hr and a kidney for 72 hr at j4-C with an application of 100 V and 500 V, respectively.…”

Section: Discussionsupporting

confidence: 64%

“…Several organ preservation experiments at subzero temperatures have been reported using cryoprotectants such as polyethylene glycol, 2,3-butanediol, or antifreeze protein (5Y7). On the other hand, other groups have reported subzero organ preservation using a special supercooling refrigerator (8,9). However, the functional advantage of heart preservation in these refrigerators has not been reported.…”

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confidence: 99%

Subzero 24-hr Nonfreezing Rat Heart Preservation

Kato

Tomita²,

Yamaguchi³

et al. 2012

Transplantation

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“…for transplantation from less than 6 h to 21 h [19], [20,21]. Okamoto et al show that preservation of a lung in a supercooled state at À2 C, has substantial benefits over the preservation at 4 C [22]. Lungs stored using a method of lung preservation in which electric fields are facilitate supercooling, show better organ function after preservation at À2 C than conventional storage at 4 C. A recent paper has shown that liver can be transplanted after four days of preservation at À6 C with perfusion supercooling [23].…”

Section: Methodsmentioning

confidence: 99%

The nematode Caenorhabditis elegans survives subfreezing temperatures in an isochoric system

Mikus

Miller

Năstase

et al. 2016

Biochemical and Biophysical Research Communications

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TitleThe nematode Caenorhabditis elegans survives subfreezing temperatures in an isochoric system a b s t r a c tThis study is the first experimental evidence showing that a living multicellular organism, the nematode Caenorhabditis elegans, can survive subfreezing temperatures in an isochoric (constant volume) thermodynamic system, while immersed in a simple isotonic solution, without the addition of cryoprotectants. Some of the test conditions were more extreme than those found at the ice/water interface of the Antarctic subglacial Vostok lake. On earth, life takes place in an isobaric (constant pressure) environment. In isobaric systems, subfreezing temperature survival of organisms in nature and subfreezing temperature preservation of living material for biotechnology and medicine, is made possible by use of cryoprotective chemicals additives. Our theoretical thermodynamic studies suggested that in an isochoric system, living biological material could survive subfreezing temperatures, without any cryoprotective chemicals. By confirming the theoretical predictions, this paper suggests a new technology for subfreezing preservation of cells, organs and organisms of possible value for biotechnology and medicine as well as new possible mechanisms of living organism survival in nature.

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446: Successful Subzero Non-Freezing Preservation of Rat Lungs with Supercooling Technology

Cited by 3 publications

References 0 publications

Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model

Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model

Subzero 24-hr Nonfreezing Rat Heart Preservation

The nematode Caenorhabditis elegans survives subfreezing temperatures in an isochoric system

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