45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity

Abstract

In the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass(®)) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass(®)/SeNp and 45S5Bioglass(®)/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.

Poly (lactic acid)-based biomaterials for orthopaedic regenerative engineering

“…In addition to silver nanoparticles that have been widely studied for anti-bacterial/microbial applications, graphene oxide [208] and selenium nanoparticles have been demonstrated to be excellent candidates for this purpose [211, 212]. One recent study indicated a significant decrease in long term proliferation of osteoblast-like sarcoma cells, while the proliferation of osteoblasts were two times higher, indicating the dual purpose of these nanoparticles.…”

Controlled release of drugs in electrosprayed nanoparticles for bone tissue engineering

“…Stevanovic et al prepared uniform, amorphous, stable selenium nanoparticles immobilized inside spherical PLGA particles (PLGA/Se). Bioactive glass based constructs coated with PLGA/Se nanoparticles using foam replica method proved to be a promising candidate in bone tissue repair and rejuvenation by promoting bone regeneration and eliminating possible infections and inflammations[58]. In another study, nanosized mesoporous bioactive glass (NMBG) nanoparticles were prepared and coated onto PLGA-calcium silicate scaffolds for better mechanical strength, drug delivery and bioactivity in BTE.…”

Polyurethane/58S bioglass nanofibers: synthesis, characterization, and in vitro evaluation

“…Several studies have shown that BG is angiogenic, 16 osteoconductive, 17,18 and osteoinductive. 19 BG also supports osteoblasts, which are vital for adhesion, growth, differentiation, and new bone formation in bone tissue engineering.…”