5-Aminolevulinic acid modulates antioxidant defense systems and mitigates drought-induced damage in Kentucky bluegrass seedlings

Niu, Kuiju; Ma, Xiaopeng; Liang, Guoling; Ma, Haiching; Zhou, Jia; Liu, Wenhui; Qianqian, Yu

doi:10.1007/s00709-017-1101-4

Cited by 37 publications

(24 citation statements)

References 44 publications

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“…The promotive effect of ALA on plant drought tolerance was first reported in wheat (Al‐Thabet ) and barley (Al‐Khateeb ), and then tobacco (Cheng et al ). Thereafter, ALA‐promoted drought tolerance of Glycyrrhiza uralensis (Zhang et al ), Sarcandra glabra (Zhang et al ), Fagopyrum dibotrys (Wei et al ), Banana (He et al ), Brassica napus (Liu et al ), Dalbergia odorifera (Wang et al ), Leymus chinensis (Song et al ) and Poa pratensis (Niu et al ) were successively reported. However, this effect of ALA is still very mystery because many studies have shown that ALA promotes leaf stomatal opening and increases transpiration (Wang et al ; Chen et al ; An et al ).…”

Section: Discussionmentioning

confidence: 99%

Exogenous 5‐aminolevulinic acid improves strawberry tolerance to osmotic stress and its possible mechanisms

Cai

et al. 2019

Physiologia Plantarum

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Cultivated strawberry, one of the major fruit crops worldwide, is an evergreen plant with shallow root system, and thus sensitive to environmental changes, including drought stress. To investigate the effect of 5-aminolevulinic acid (ALA), a new environment-friendly plant growth regulator, on strawberry drought tolerance and its possible mechanisms, we treated strawberry (Fragaria × annanasa Duch. cv. 'Benihoppe') with 15% polyethylene glycol 6000 to simulate osmotic stress with or without 10 mg l −1 ALA. We found that ALA significantly alleviated PEG-inhibited plant growth and improved water absorption and xylem sap flux, indicating ALA mitigates the adverse effect of osmotic stress on strawberry plants. Gas exchange and chlorophyll fluorescence analysis showed that ALA mitigated PEG-induced decreases of P n , G s , T r , P n /C i , photosystem I and II reaction center activities, electron transport activity, and photosynthetic performance indexes. Equally important, ALA promoted PEG-increased antioxidant enzyme activities and repressed PEG-increased malondialdehyde and superoxide anion in both leaves and roots. Specially, ALA repressed H 2 O 2 increase in leaves, but stimulated it in roots. Furthermore, ALA repressed abscisic acid (ABA) biosynthesis and signaling gene expressions in leaves, but promoted those in roots. In addition, ALA blocked PEG-downregulated expressions of plasmalemma and tonoplast aquaporin genes PIP and TIP in both leaves and roots. Taken together, ALA effectively enhances strawberry drought tolerance and the mechanism is related to the improvement of water absorption and conductivity. The tissue-specific responses of ABA biosynthesis, ABA signaling, and H 2 O 2 accumulation to ALA in leaves and roots play key roles in ALA-improved strawberry tolerance to osmotic stress.

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Section: Discussionmentioning

confidence: 99%

Exogenous 5‐aminolevulinic acid improves strawberry tolerance to osmotic stress and its possible mechanisms

Cai

et al. 2019

Physiologia Plantarum

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“…5‐aminolevulinic acid (ALA) is a key precursor in tetrapyrrole biosynthesis in all organisms, such as chlorophyll and heme in plants. It has been recognized as a vital plant growth regulator due to its various roles in several key physiological processes such as promoting plant growth and conferring abiotic stress tolerance in plants (Liu et al , Xu et al , Niu et al , Sun et al , Akram et al , Liu et al , Wu et al ). Increasing attention has been paid to ALA because of its environmental‐friendly characteristic and great application potential in agriculture (Wang et al , Akram and Ashraf ) and horticulture (Feng et al , An et al , Zheng et al , An et al , Zheng et al ).…”

Section: Introductionmentioning

confidence: 99%

“…Introduction 5-aminolevulinic acid (ALA) is a key precursor in tetrapyrrole biosynthesis in all organisms, such as chlorophyll and heme in plants. It has been recognized as a vital plant growth regulator due to its various roles in several key physiological processes such as promoting plant growth and conferring abiotic stress tolerance in plants , Niu et al 2017, Sun et al 2017, Akram et al 2018, Liu et al 2018, Wu et al 2018. Increasing attention has been paid to ALA because of its environmental-friendly Abbreviations -ABA, abscisic acid; ALA, 5-aminolevulinic acid; AsA, ascorbic acid; BR, brassinosteroid; CalA, calyculin A; CAT, catalase; DPI, diphenylene iodonium; GFP-TUA6, green fluorescent protein-tagged -tubulin 6 transgenic Arabidopsis; H 2 O 2 , hydrogen peroxide; MeJA, methyl jasmonate; MS, Murashige and Skoog; MTs, microtubules; OA, okadaic acid; OE, overexpression; PP2A, type 2A protein phosphatase; PP2A-C2, C2 subunit of type 2A protein phosphatase; PP2A-C2-OE, PP2A-C2 overexpression; PPFD, photosynthetic photo flux density; ROS, reactive oxygen species; VBT, vinblastine. characteristic and great application potential in agriculture (Wang et al 2003, Akram andAshraf 2013) and horticulture (Feng et al 2016, An et al 2016b, Zheng et al 2017, Zheng et al 2018.…”

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confidence: 99%

PP2A and microtubules function in 5‐aminolevulinic acid‐mediated H₂O₂ signaling in Arabidopsis guard cells

Xiong

Shu

et al. 2019

Physiologia Plantarum

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5‐aminolevulinic acid (ALA), a plant growth regulator with great application potential in agriculture and horticulture, induces stomatal opening and inhibits stomatal closure by decreasing guard cell H2O2. However, the mechanisms behind ALA‐decreased H2O2 in guard cells are not fully understood. Here, using type 2A protein phosphatase (PP2A) inhibitors, microtubule‐stabilizing/disrupting drugs and green fluorescent protein‐tagged α‐tubulin 6 transgenic Arabidopsis (GFP‐TUA6), we find that PP2A and cortical microtubules (MTs) are involved in ALA‐regulated stomatal movement. Then, we analyze stomatal responses of Arabidopsis overexpressing C2 catalytic subunit of PP2A (PP2A‐C2) and pp2a‐c2 mutant to ALA and abscisic acid (ABA) under both light and dark conditions, and show that PP2A‐C2 participates in ALA‐induced stomatal movement. Furthermore, using pharmacological methods and confocal studies, we reveal that PP2A and MTs function upstream and downstream, respectively, of H2O2 in guard cell signaling. Finally, we demonstrate the role of H2O2‐mediated microtubule arrangement in ALA inhibiting ABA‐induced stomatal closure. Our findings indicate that MTs regulated by PP2A‐mediated H2O2 decreasing play an important role in ALA guard cell signaling, revealing new insights into stomatal movement regulation.

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“…Puyang et al (2015) reported high degradation of the chloroplast after ROS generation, which was attributed to the chloroplast being the principal site of ROS production. Niu et al (2017) reported the production of MDA, formed from lipid oxidation, as an indication of cell membrane damage due to ROS accumulation.…”

Section: Introductionmentioning

confidence: 99%

Physiological Responses to Hypoxia and Manganese in Eucalyptus Clones with Differential Tolerance to Vale do Rio Doce Shoot Dieback

Harguindeguy

Castro

Novais

et al. 2018

Rev. Bras. Ciênc. Solo

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ABSTRACT:Vale do Rio Doce shoot dieback (VRDSD) is an anomaly whose cause seems to be associated with hypoxic conditions and their consequences (excess Mn and Fe) triggered by elevation of the water table in areas with poor drainage. Different plants have distinct survival strategies under this form of stress. The objective of this study was to understand the physiological responses involved in the differential tolerance of eucalyptus clones to VRDSD and their relationship to hypoxia and excess Mn. A hydroponic experiment was carried out using a 2 × 2 × 2 factorial arrangement, two eucalyptus clones with different levels of tolerance to VRDSD (sensitive Urograndis hybrid -1213; and the tolerant Rio Claro hybrid -Eucalyptus grandis x unknown -2719), two concentrations of O 2 (8 and 4 mg L -1 ), and two Mn concentrations (1.39 and 300 mg L -1 ) in a randomized block design (RBD) with three replicates. Forty-day-old clones were maintained in Clark nutrient solution for 30 days. After this period, the treatments were applied for 11 days. Plant gaseous exchange shoot and root production, and the quantity of enzymes related to oxidative stress in leaves and roots were evaluated. In the tolerant clone, reactive oxygen species (ROS) were produced under hypoxic conditions, accompanied by reduction in production of dry matter, malondialdehyde (MDA), and in activity of the enzyme alcohol dehydrogenase (ADH). However, this clone had greater production of superoxide dismutase (SOD) under these conditions, an enzyme responsible for detoxification of ROS, which acts as part of the Low Oxygen Quiescence Syndrome (LOQS). In contrast, sensitive clones did not exhibit expressive reductions in growth or changes in the leaf/root ratio. These clones formed large quantities of adventitious roots and had high levels of MDA and ADH and low levels of SOD. Therefore, sensitive clones appear not to be prepared for detoxification of ROS and other toxic metabolites, but rather adopt morphological escape mechanisms, the Low Oxygen Escape Syndrome (LOES), in response to hypoxia. Thus, the period of soil waterlogging may cause the death of large numbers of roots in sensitive clones, limiting their ability to absorb water and nutrients and culminating in the death of these plants. Excess Mn seems to aggravate the damage caused by hypoxia, but it is not the causal agent of VRDSD.

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5-Aminolevulinic acid modulates antioxidant defense systems and mitigates drought-induced damage in Kentucky bluegrass seedlings

Cited by 37 publications

References 44 publications

Exogenous 5‐aminolevulinic acid improves strawberry tolerance to osmotic stress and its possible mechanisms

Exogenous 5‐aminolevulinic acid improves strawberry tolerance to osmotic stress and its possible mechanisms

PP2A and microtubules function in 5‐aminolevulinic acid‐mediated H₂O₂ signaling in Arabidopsis guard cells

Physiological Responses to Hypoxia and Manganese in Eucalyptus Clones with Differential Tolerance to Vale do Rio Doce Shoot Dieback

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5-Aminolevulinic acid modulates antioxidant defense systems and mitigates drought-induced damage in Kentucky bluegrass seedlings

Cited by 37 publications

References 44 publications

Exogenous 5‐aminolevulinic acid improves strawberry tolerance to osmotic stress and its possible mechanisms

Exogenous 5‐aminolevulinic acid improves strawberry tolerance to osmotic stress and its possible mechanisms

PP2A and microtubules function in 5‐aminolevulinic acid‐mediated H2O2 signaling in Arabidopsis guard cells

Physiological Responses to Hypoxia and Manganese in Eucalyptus Clones with Differential Tolerance to Vale do Rio Doce Shoot Dieback

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PP2A and microtubules function in 5‐aminolevulinic acid‐mediated H₂O₂ signaling in Arabidopsis guard cells