5-Aza-2′-deoxycytidine treatment induces skeletal myogenic differentiation of mouse dental pulp stem cells

Nakatsuka, Ryusuke; Nozaki, Tadashige; Uemura, Yukari; Matsuoka, Yoshikazu; Sasaki, Yutaka; Shinohara, Mitsuko; Ohura, Kiyoshi; Sonoda, Yoshiaki

doi:10.1016/j.archoralbio.2010.03.003

Cited by 62 publications

(60 citation statements)

References 35 publications

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“…Recently, DPSCs have become a rapidly developing technology, which can be used in biology and regenerative medicine. DPSCs have the potential to differentiate into several lineages including osteogenic, adipocyte, muscle, neurons and cartilage (Gronthos et al 2000;Lee et al 2013;Nakatsuka et al 2010;Arthur et al 2008). Comparison of their multipotency with bone marrow stem cells (BMSCs), has demonstrated that proliferation, availability, and cell number of DPSCs were more elevated than for bone marrow MSCs (Huang et al 2009;Alge et al 2010).…”

Section: Introductionmentioning

confidence: 99%

The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway

et al. 2016

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Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the selfrenewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 family cytokines, inhibits adipogenic differentiation and stimulates osteogenic differentiation of human bone marrow mesenchymal stem cells. However, the effect of OSM on DPSCs is unclear. We found that OSM induced osteogenic differentiation of DPSCs, promoting matrix mineralization as measured by Alizarin Red S staining. OSM also increased expression of osteogenesis-associated gene products Alkaline phosphatase, Bone morphogenetic protein 2 (BMP2), Runt-related transcription factor 2 and Osteocalcin (OCN) as assessed by immunoblotting. We also found that OSM activated the Signal Transducer And Activator Of Transcription 3 (STAT3) pathway during the osteogenic differentiation of DPSCs. Blocking the osteogenic differentiation by silencing of STAT3 can significantly inhibit OSMinduced osteogenic differentiation of DPSCs and the expression of related genes, furthermore matrix mineralization was also suppressed. In summary, OSM promotes osteoblastic differentiation of DPSCs and osteogenesis-related genes expression through the JAK3/STAT3 signaling pathway which may be useful for the autologous transplantation of DPSCs.

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Section: Introductionmentioning

confidence: 99%

The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway

et al. 2016

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“…Dental pulp is a nonhematopoietic tissue, although blood vessels are extended into the dental pulp. Previous studies have reported that MSC-like dental pulp stem cells (dental pulp-derived MSCs, DPSCs) exist in the dental pulp (16,25). One of the major roles of DPSCs is thought to be their ability to supply hard tissue components by differentiating into odontoblasts (10).…”

Section: Introductionmentioning

confidence: 99%

Mouse Dental Pulp Stem Cells Support Human Umbilical Cord Blood-Derived Hematopoietic Stem/Progenitor Cells in Vitro

Nakatsuka

Matsuoka

Uemura³

et al. 2015

Cell Transplant

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It is well documented that specialized mesenchymal stem/stromal cells (MSCs) constitute the hematopoietic stem cell (HSC) niche in the bone marrow (BM), and these MSCs support/maintain the HSCs in an undifferentiated state. A number of studies have demonstrated that BM-derived MSCs (BM-MSCs) can support HSCs in vitro. However, it remains unclear whether nonhematopoietic tissue-derived MSC-like cells, such as dental pulp stem cells (DPSCs), have the ability to support HSCs. In this study, we prospectively isolated DPSCs from mouse mandibular incisors by fluorescence-activated cell sorting (FACS) using BM-MSC markers, such as PDGFRa and Sca-1. The PDGFRa and Sca-1 double-positive DPSCs and BM-MSCs showed similar morphologies and expression patterns of MSC markers. The ability of the DPSCs to support hematopoietic stem/progenitor cells (HSPCs) was then analyzed by an in vitro coculture system. Moreover, their HSC-supporting activity was evaluated by in vivo xenotransplantation assays using NOD/Shi-scid/IL-2Rg c null (NOG) mice. Interestingly, the DPSCs supported human cord blood (CB)-derived CD34-positive (CD34

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“…We also demonstrated the possibility that these cells could differentiate into a glial lineage by detecting the expression of Gfap, Nes, Mtap2, and Tubb3 (10). We recently reported the MAPC-like differentiation potential of mouse dental pulp-derived cells, suggesting that DNA demethylation might trigger myogenic differentiation based on myosin heavy chain expression and myotube formation following 5-aza-2′-deoxycytidine treatment of these mouse cells (11). To further evaluate the multipotency of the cells and to identify the genes regulated during differentiation, we examined the in vitro differentiation of these cells into an adipocyte lineage.…”

Section: Rps18mentioning

confidence: 76%

Gene Expression Profile of Dental Pulp Cells During Differentiation Into an Adipocyte Lineage

Nozaki¹,

Ohura²

2011

J Pharmacol Sci

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Abstract. Gene regulation during in vitro differentiation into adipocytes was examined in rat dental pulp-derived cells. Insulin, 3-isobutyl-1-methylxanthine, and dexamethasone were added to induce adipogenesis. Cells containing lipid droplets were observed after induction as in 3T3 L1 cells. Rat dental pulp-derived cells showed their potential to differentiate into adipocytes in vitro. In both types of cells, the pluripotent markers Oct-3/4 and Sox2 were downregulated during differentiation, whereas the expression of Nanog was not significantly changed during differentiation. Interestingly, in the dental pulp-derived cells, the level of Oct-3/4 was transiently induced at 1 week after induction and then significantly decreased during differentiation. Based on the expression profiles determined using GeneChip Arrays, 3418 probes across 10 clusters showed a difference in expression at 1, 2, and 3 weeks after induction versus before induction. Notably, genes in the PPAR signaling pathway including Pparγ, Fabp4, and the C/EBP family were upregulated by more than 3-fold. Upregulation of the PPAR pathways seems to be a critical signal transduction pathway in this differentiation system. These findings indicate that dental pulp-derived cells are a potential source of adipogenic cells, and their gene expression profile could be useful in future regenerative medicine applications.

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5-Aza-2′-deoxycytidine treatment induces skeletal myogenic differentiation of mouse dental pulp stem cells

Cited by 62 publications

References 35 publications

The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway

The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway

Mouse Dental Pulp Stem Cells Support Human Umbilical Cord Blood-Derived Hematopoietic Stem/Progenitor Cells in Vitro

Gene Expression Profile of Dental Pulp Cells During Differentiation Into an Adipocyte Lineage

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