2005
DOI: 10.1111/j.1460-9568.2005.04504.x
|View full text |Cite
|
Sign up to set email alerts
|

5‐Bromo‐2′‐deoxyuridine is selectively toxic to neuronal precursors in vitro

Abstract: The effect of 5-bromo-2'-deoxyuridine (BrdU) incorporation on the phenotype of progeny derived from expanded E18 rat striatal precursors was examined. BrdU was administered to cultures for 24 h prior to differentiation. Results revealed that there was selective toxicity of this compound to developing TuJ1+ neurons, but not glia, at concentrations used in most labelling studies. Therefore, a BrdU dose-response curve from 0.2 microM to 10 microM was established. The optimum dose of BrdU for labelling cells was 0… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

3
44
1
3

Year Published

2006
2006
2020
2020

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 45 publications
(51 citation statements)
references
References 29 publications
(32 reference statements)
3
44
1
3
Order By: Relevance
“…There have been suggestions that the former may be toxic to dividing cells (Caldwell et al, 2005), but we find that there is a high correlation between this marker and Ki-67. Experiments 2 and 3 therefore relied on Ki-67 labelling alone to detect dividing progenitor cells.…”
Section: Discussionmentioning
confidence: 49%
“…There have been suggestions that the former may be toxic to dividing cells (Caldwell et al, 2005), but we find that there is a high correlation between this marker and Ki-67. Experiments 2 and 3 therefore relied on Ki-67 labelling alone to detect dividing progenitor cells.…”
Section: Discussionmentioning
confidence: 49%
“…This could be a possible explanation for ORNs death in the OE and the subsequent decrease in OMP labeling. Caldwell et al [27] have observed an increase in the number of apoptotic cells in cultures of rat neuronal progenitor cells after treatment with increasing BrdU concentrations. The neuronal population decays to 30% when compared to controls and the decrease is proportional to the BrdU concentration [27].…”
Section: Discussionmentioning
confidence: 97%
“…Caldwell et al [27] have observed an increase in the number of apoptotic cells in cultures of rat neuronal progenitor cells after treatment with increasing BrdU concentrations. The neuronal population decays to 30% when compared to controls and the decrease is proportional to the BrdU concentration [27]. We obtained a similar result, with 10 mM BrdU detecting an increase in the number of apoptotic cells measured by aCasp-3 and TUNEL.…”
Section: Discussionmentioning
confidence: 97%
“…For those rats, starting on Day 13, intraperitoneal (ip) BrdU injections were given twice per week (Mondays and Thursdays) through Day 37 (see Table 1). In order to determine whether BrdU injections themselves produced changes in other cell markers, a subset of rats from each group did not receive BrdU injections [3,5].…”
Section: Quantity and Phenotype Of Newly Born Cellsmentioning
confidence: 99%