2011
DOI: 10.1016/j.bmc.2011.08.035
|View full text |Cite
|
Sign up to set email alerts
|

5-Fluoro-4-thiouridine phosphoramidite: New synthon for introducing photoaffinity label into oligodeoxynucleotides

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

1
13
0

Year Published

2012
2012
2024
2024

Publication Types

Select...
4
1
1

Relationship

2
4

Authors

Journals

citations
Cited by 12 publications
(14 citation statements)
references
References 29 publications
1
13
0
Order By: Relevance
“…Consistent with our previous observations,14 irradiation of duplex D1 resulted in the concomitant disappearance of signals for both the modified oligonucleotide ( ODN1 ) and the unmodified complementary strand ( ODN4 ) and the formation of one major ( 1 b ) and one minor ( 1 a ) fluorescent photoproduct (Figure 1). Complete conversion of ODN1 was observed within 1 min of irradiation.…”
Section: Methodssupporting
confidence: 92%
See 2 more Smart Citations
“…Consistent with our previous observations,14 irradiation of duplex D1 resulted in the concomitant disappearance of signals for both the modified oligonucleotide ( ODN1 ) and the unmodified complementary strand ( ODN4 ) and the formation of one major ( 1 b ) and one minor ( 1 a ) fluorescent photoproduct (Figure 1). Complete conversion of ODN1 was observed within 1 min of irradiation.…”
Section: Methodssupporting
confidence: 92%
“…Based on the observation of a fluorophore created by photo‐crosslinking it was concluded that in order to induce such a reaction in a DNA duplex, FS U should be placed between two adenosines. As we have previously demonstrated, selective photoexcitation of the FS U residue in an oligodeoxynucleotide duplex with an A FS U A motif in one strand leads to fluorescent interstrand crosslink formation with one of the thymines of TAT in the complementary target strand 14. The interstrand photoadducts of FS U and T are identical to those formed when monomeric FS U is irradiated in the presence of thymidine.…”
Section: Methodsmentioning
confidence: 60%
See 1 more Smart Citation
“…13 C ribose-labelled phosphoramidites were purchased from Pitsch Nucleic Acids. 4thiouridine phosphoramidites were synthesised as described previously 76,77 . All other chemicals were obtained from Sigma-Aldrich, Fluorochem, TCI, and Fisher Scientific.…”
Section: Preparation Of Rnamentioning
confidence: 99%
“…This methodology was previously successfully applied for the synthesis of an electron deficient 4-thiopyrimidine nucleoside. 35 The silyl-protected nucleoside 17 was reacted with The synthesis of 2-methyl-2′-deoxynebularine phosphoramidite 33 (Scheme 4) started from 2-methylpurine (29). Commercially available 5-amino-4,6-dichloro-2-methylpyrimidine (26) was converted to 4,5-diamino-6-chloro-2-methylpyrimidine (27), which, after treatment with triethyl orthoformate, afforded the compound 28.…”
Section: ■ Introductionmentioning
confidence: 99%