[51] Mitochondrial ATPase inhibitor: Properties and applications

Chemical and Chemiosmotic Aspects of Electron Transport-Linked Phosphorylation

“…Studies in our laboratory (87,88) of the quantitative relationship between oligomycin-induced respiratory control and energy-linked enhancement of ANS fl uorescence and binding in submitochondrial particles have given results consistent with the conclusion that the three energy-coupling sites of the respiratory chain are located in differe . q t environments of the mem brane.…”

ATP synthesis catalyzed by the mitochondrial F1‐F0 ATP synthase is not a reversal of its ATPase activity

“…The central issue of this report concerns the questionable identity of the molecular events during ATP synthesis and hydrolysis by Ft-F 0 ATPase/synthase. It worth noting that F1 ATPase protein inhibitor (IF1) which is an intrinsic part of the mammalian ATP synthase complex is also known to be a 'unidirectional' inhibitor of ATP hydrolysis [39]. Although ApH+ dependence of FI F0-IF~ interaction have been proposed to explain the lack of inhibitory effect of IF, on oxidative phosphorylation [40], such explanation is hard to reconcile with our more recently reported data [41].…”

10N‐Nonyl acridine orange interacts with cardiolipin and allows the quantification of this phospholipid in isolated mitochondria

“…Cardiolipin, one of the three major phospholipids present in the inner membrane [8 -121, has been reported to be essential for the activity of the ADP/ATP carrier protein [I 31, for the phosphate transport protein [14] and for various other enzyme complexes [15,16]. This phospholipid has also been reported to be associated with the F1-FO ATPase [17]. Consequently, the NAO inhibition of the inner-mitochondrial-membrane enzymes [ 181 may be due to the interaction between the positively charged dye and cardiolipin, the main acidic phospholipid present in the inner mitochondrial membrane.…”