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Chen, Kuo-Cheng; Lin, Yun-Huin; Tsai, Chao-Ming; Hsieh, Cheng-Hsiang; Houng, Jer-Yiing

doi:10.1023/a:1014553109425

Cited by 31 publications

(8 citation statements)

References 9 publications

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“…Also, increase in biomass was observed till day 7 after which decrease in biomass and compactin production was observed. The glycerol had been preferably utilized for production of compactin [22] and clavulanic acid [23,24]. In clavulanic acid biosynthesis, it had been observed that a glycerol concentration higher than 2% was inhibitory [25] under submerged fermentation conditions, which had not been the case in present study under SSF conditions.…”

Section: Discussioncontrasting

confidence: 48%

Compactin Production Studies Using Penicillium brevicompactum Under Solid-State Fermentation Conditions

Shaligram

Singh

Pandey

et al. 2008

Appl Biochem Biotechnol

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In the present study, compactin production by Penicillium brevicompactum WA 2315 was optimized using solid-state fermentation. The initial one factor at a time approach resulted in improved compactin production of 905 microg gds(-1) compared to initial 450 microg gds(-1). Subsequently, nutritional, physiological, and biological parameters were screened using fractional factorial and Box-Behnken design. The fractional factorial design studied inoculum age, inoculum volume, pH, NaCl, NH(4)NO(3), MgSO(4), and KH(2)PO(4). All parameters were found to be significant except pH and KH(2)PO(4). The Box-Behnken design studied inoculum volume, inoculum age, glycerol, and NH(4)NO(3) at three different levels. Inoculum volume (p = 0.0013) and glycerol (p = 0.0001) were significant factors with greater effect on response. The interaction effects were not significant. The validation study using model-defined conditions resulted in an improved yield of 1,250 microg gds(-1) compactin. Further improvement in yield was obtained using fed batch mode of carbon supplementation. The feeding of glycerol (20% v/v) on day 3 resulted in further improved compactin yield of 1,406 microg gds(-1). The present study demonstrates that agro-industrial residues can be successfully used for compactin production, and statistical experiment designs provide an easy tool to improve the process conditions for secondary metabolite production.

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Section: Discussioncontrasting

confidence: 48%

Compactin Production Studies Using Penicillium brevicompactum Under Solid-State Fermentation Conditions

Shaligram

Singh

Pandey

et al. 2008

Appl Biochem Biotechnol

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“…In fact, these variables were shown to be the most influencing CA production by other strains [8,[12][13][14][15]. Although such an isolate was not yet characterized, its high performance in CA production with respect to common S. clavuligerus strains constitutes the main novelty of this study.…”

Section: Introductionmentioning

confidence: 94%

“…The types of nutrients, their concentrations, and the operating conditions have different effects on the accumulation of metabolites, which is controlled by intracellular effectors [7][8][9]. Selection of the most suited culture medium composition is of primary importance to increase the productivity of any bioprocess.…”

Section: Introductionmentioning

confidence: 99%

“…By a fractional factorial design, the significant factors and their effects can be studied using only a few experiments, thus reducing the operating costs [11]. Most of previous studies were devoted to the improvement of CA production by S. clavuligerus [6], by selection of the optimum operating conditions, namely pH [12], temperature [13], agitation [14], concentrations of glycerol as a carbon source [8], and of soybean flour as a nitrogen source [15].…”

Section: Introductionmentioning

confidence: 99%

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Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Viana

Carneiro-Cunha

Araújo

et al. 2009

Appl Biochem Biotechnol

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Clavulanic acid (CA) is a beta-lactam antibiotic, which has a potent beta-lactamase inhibiting activity. The influence of five variables, namely pH (6.0, 6.4, and 6.8), temperature (28 degrees C, 30 degrees C, and 32 degrees C), agitation intensity (150, 200, and 250 rpm), glycerol concentration (5.0, 7.5, and 10 g/L) and soybean flour concentration (5.0, 12.5, and 20 g/L), on CA production by a new isolate of Streptomyces (DAUFPE 3060) was investigated in 250-mL Erlenmeyer flasks using a fractional factorial design. Temperature and soybean flour concentration were shown to be the two variables that exerted the most important effects on the production of CA at 95% confidence level. The highest CA concentration (494 mg/L) was obtained after 48 h at 150 rpm, 32 degrees C, pH 6.0, 5.0 g/L glycerol, and 20 g/L soybean flour concentrations. Under these conditions, the yields of biomass and product on consumed substrate were 0.26 g(X)/g(S) and 64.3 mg(P)/g(S), respectively. Fermentations performed in 3.0-L bench-scale fermenter allowed increasing the CA production by about 60%.

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“…These factors include components of the culture medium and/or intermediate compounds of primary metabolism, which in turn participate directly or indirectly in product biosynthesis [25]. Glycerol, for example, is preferentially used as carbon source forCAproduction [5,26], whereas starch is more suitable for the production of cephamycin C [2,25,27]. The regulation of cephamycin C and CA production are interconnected by the pathway specific regulatory gene Ccar [28].…”

Section: Culture Media For Clavulanic Acid Production By the Strain Smentioning

confidence: 99%

Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis

Pinilla

Toro

Rossa

et al. 2018

DYNA

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The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were tested in different culture media, using product yields and culture media costs as selection criteria. On average, ScATCC reached a concentration of CA 1.6-fold higher than ScDSM, using the isolated soybean protein (ISP) culture medium. A central composite experimental design was used to optimize the medium, by studying the influence of ISP and K2HPO4 concentration on the production of CA. The maximum CA concentration obtained in this optimized medium was 56 mg·L -1 , close to the theoretical optimum. Our results confirm that a culture medium containing a rich carbon source such as glycerol and/or starch provides the best conditions for CA production.Keywords: clavulanic acid; culture media design; strain selection; Streptomyces clavuligerus; antibiotics.Selección de una cepa de Streptomyces clavuligerus para la biosíntesis de ácido clavulánico: un estudio basado en los efectos de la composición del medio de cultivo y análisis estadístico Resumen La importancia clínica e industrial del ácido clavulánico (AC) promueve la búsqueda de nuevas estrategias para aumentar su productividad a un menor costo. En esta contribución, se evaluó la producción de AC en dos cepas de referencia de Streptomyces clavuligerus (Sc), ATCC27064 y DSM41826; éstas se probaron en diferentes medios de cultivo; los rendimientos de producto y costo de los medios se usaron como criterios de selección. En promedio, ScATCC alcanzó una concentración de AC 1.6-veces mayor que ScDSM, en el medio de proteína de soya (ISP). Se optimizó el medio con un diseño experimental central compuesto, estudiando la influencia del ISP y K2HPO4 sobre la producción de AC. La concentración máxima de AC obtenida en el medio optimizado (56mg·L -1 ) estuvo cerca del óptimo teórico. Nuestros resultados confirman que medios de cultivo con fuentes de carbono como glicerol y/o almidón proporcionan las mejores condiciones para la producción de AC.Palabras clave: ácido clavulánico; diseño de medios de cultivo; selección de cepa; Streptomyces clavuligerus; antibióticos.

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Untitled

Cited by 31 publications

References 9 publications

Compactin Production Studies Using Penicillium brevicompactum Under Solid-State Fermentation Conditions

Compactin Production Studies Using Penicillium brevicompactum Under Solid-State Fermentation Conditions

Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis

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