Compactin Production Studies Using Penicillium brevicompactum Under Solid-State Fermentation Conditions

Abstract

In the present study, compactin production by Penicillium brevicompactum WA 2315 was optimized using solid-state fermentation. The initial one factor at a time approach resulted in improved compactin production of 905 microg gds(-1) compared to initial 450 microg gds(-1). Subsequently, nutritional, physiological, and biological parameters were screened using fractional factorial and Box-Behnken design. The fractional factorial design studied inoculum age, inoculum volume, pH, NaCl, NH(4)NO(3), MgSO(4), and KH(2)PO(4). All parameters were found to be significant except pH and KH(2)PO(4). The Box-Behnken design studied inoculum volume, inoculum age, glycerol, and NH(4)NO(3) at three different levels. Inoculum volume (p = 0.0013) and glycerol (p = 0.0001) were significant factors with greater effect on response. The interaction effects were not significant. The validation study using model-defined conditions resulted in an improved yield of 1,250 microg gds(-1) compactin. Further improvement in yield was obtained using fed batch mode of carbon supplementation. The feeding of glycerol (20% v/v) on day 3 resulted in further improved compactin yield of 1,406 microg gds(-1). The present study demonstrates that agro-industrial residues can be successfully used for compactin production, and statistical experiment designs provide an easy tool to improve the process conditions for secondary metabolite production.

Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus

“…RSM is a sequential procedure with an initial objective to lead the experimenter rapidly and efficiently along a path of improvement toward the general vicinity of the optimum. Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing mevastatin production [10] , [11] , [12] , [1] , [2] , [3] . 2 5 full factorial central composite design and RSM were applied to determine the optimal for each significant variable.…”

Comparative studies on the production of statins using different microbial strains

“…The production of mevastatin by P. brevicompactum WA 2315 has been previously optimized using SSF (Shaligram et al, 2009). The feeding of glycerol (20% v/v) into the growth medium on day 3 resulted in further improvement of mevastatin yield to 1406 μg/gds.…”

Microbial surfactants: Fundamentals and applicability in the formulation of nano-sized drug delivery vectors

“…This is due to changes in the physical membrane structure or through the disruption of protein conformations which alter important membrane functions such as transport and energy generation [22]. The molecular mechanisms of surfactin interactions with membrane structures were described by [40,27]. An important step for membrane destabilization and leakage is the dimerization of surfactin into the bilayer [41].…”