[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe

Moreno, Sergio; Klar, Amar J. S.; Nurse, Paul

doi:10.1016/0076-6879(91)94059-l

Cited by 3,596 publications

(3,561 citation statements)

References 23 publications

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“…Standard media and genome engineering methods for fission yeast were used as described previously (Moreno et al, 1991). Strains and plasmids used in this study are listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

The unfolded protein response in fission yeast modulates stability of select mRNAs to maintain protein homeostasis

Kimmig

Diaz

Zheng

et al. 2012

eLife

134

162

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The unfolded protein response (UPR) monitors the protein folding capacity of the endoplasmic reticulum (ER). In all organisms analyzed to date, the UPR drives transcriptional programs that allow cells to cope with ER stress. The non-conventional splicing of Hac1 (yeasts) and XBP1 (metazoans) mRNA, encoding orthologous UPR transcription activators, is conserved and dependent on Ire1, an ER membrane-resident kinase/endoribonuclease. We found that the fission yeast Schizosaccharomyces pombe lacks both a Hac1/XBP1 ortholog and a UPR-dependent-transcriptional-program. Instead, Ire1 initiates the selective decay of a subset of ER-localized-mRNAs that is required to survive ER stress. We identified Bip1 mRNA, encoding a major ER-chaperone, as the sole mRNA cleaved upon Ire1 activation that escapes decay. Instead, truncation of its 3′ UTR, including loss of its polyA tail, stabilized Bip1 mRNA, resulting in increased Bip1 translation. Thus, S. pombe uses a universally conserved stress-sensing machinery in novel ways to maintain homeostasis in the ER.DOI: http://dx.doi.org/10.7554/eLife.00048.001

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“…Standard media and genome engineering methods for fission yeast were used as described previously (Moreno et al, 1991). Strains and plasmids used in this study are listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

The unfolded protein response in fission yeast modulates stability of select mRNAs to maintain protein homeostasis

Kimmig

Diaz

Zheng

et al. 2012

eLife

134

162

View full text Add to dashboard Cite

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“…Diploid cells were selected on solid agar containing EMM2 medium (lacking adenine). For Agn2p expression regulation, diploid cells first were grown to exponential phase in EMM2 medium containing 1% w/v ammonium chloride (conditions that inhibit sporulation; Moreno et al, 1991), with or without 10 µM thiamine. To induce sporulation synchronously, cells were shifted to EMM2 medium containing 0.5% w/v sodium glutamate (a poor nitrogen source) and 0.5% glucose, with or without 10 µM thiamine.…”

Section: Escherichia Coli Strains and Culture Conditionsmentioning

confidence: 99%

Role of the α‐glucanase Agn2p in ascus‐wall endolysis following sporulation in fission yeast

Dekker

Rijssel

Distel

et al. 2007

Yeast

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During sporulation in the ascomyceteous fungus Schizosaccharomyces pombe, diploid cells undergo differentiation into asci containing four haploid ascospores, which are highly resistant to environmental stresses. Although the morphogenetic processes involved in ascospore formation have been studied extensively, little is known about the molecular mechanism that ensures the release of mature ascospores from the ascus, allowing their dispersal into the environment. Recently, we identified Agn2p as the paralogue of the characterized endo-(1,3)-α-glucanase Agn1p, and observed that asci deleted for agn2 are defective in ascospore dispersal. Here, we focus on the cellular and biochemical functions of Agn2p. By placing agn2 under the control of an inducible promoter, we show that expression of agn2 is required for the efficient release of ascospores from their asci. Furthermore, we characterize the enzyme activity of purified recombinant Agn2p and show that Agn2p, like Agn1p, is an endo-(1,3)-α-glucanase that produces predominantly (1,3)-α-glucan pentasaccharides. Finally, we demonstrate that exogenous addition of purified Agn2p liberated the ascospores from asci deleted for agn2. We propose that Agn2p participates in the endolysis of the ascus wall by hydrolysing its (1,3)-α-glucan, thereby assisting in the release of ascospores.

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“…Different methods were used to characterize the phenotype of paal disrupted cells (Moreno et al 1991;Ohkura & Yanagida 1991).…”

Section: Phenotypic Analysis Ofpaal Gene Disrupted Cellsmentioning

confidence: 99%

The regulatory subunits of fission yeast protein phosphatase 2A (PP2A) affect cell morphogenesis, cell wall synthesis and cytokinesis

et al. 1996

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Backggvound: Protein phosphatase 2A (PP2A) holoenzymes have a trimeric structure, consisting of a catalytic subunit C and two regulatory subunits A (PR65) and B (PR55). In fission yeast the C subunits, being 80% identical to their mammalian counterparts, are essential for viability and negatively regulate the entry into mitosis. Genetic analyses in budding yeast and Drosophila show that the regulatory subunits are implicated in chromosome segregation, cell morphogenesis and/or cytokinesis. Results:We isolated fission yeast genes p a a l + and p a b l + encoding the regulatory subunits PR65 and PR55, respectively. Gene disruption showed that the paa1'-gene was essential for viability while p a b l + was not required at 26-33°C. Microtubule

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[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe

Cited by 3,596 publications

References 23 publications

The unfolded protein response in fission yeast modulates stability of select mRNAs to maintain protein homeostasis

The unfolded protein response in fission yeast modulates stability of select mRNAs to maintain protein homeostasis

Role of the α‐glucanase Agn2p in ascus‐wall endolysis following sporulation in fission yeast

The regulatory subunits of fission yeast protein phosphatase 2A (PP2A) affect cell morphogenesis, cell wall synthesis and cytokinesis

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