561 Topical application of anacardic acid (6-nonadecyl salicylic acid) reduces UV-induced AcH3, γ-H2AX, MMP-1 and IL-6 expressions in human skin

Abstract

Our previous study showed that anacardic acid (AA) suppressed ultraviolet (UV)-induced histone modification, matrix metalloproteinase (MMP)-13, MMP-9, cyclooxygenase-2 and tumor necrosis factor-alpha expressions in hairless mice skin. To determine the photoprotective and anti-inflammatory effects of AA in human skin, the buttock of young subjects (age 21-39 yr, n¼9) was irradiated with 2 minimal erythema dose (MED) of UV, and topically treated with 0.1% AA or its vehicle after UV irradiation. Topical treatment with AA did not only reduce UV-induced increases in erythema index as measured by Mexameter TM , but also prevented UV-induced increased expression of MMP-1 and interleukin-6. In addition, AA inhibited UV-induced expression of acetyl-H3 and g-H2AX, a DNA damage marker. Our data suggest that the topical application of AA, an inhibitor of p300 histone acetyltransferase, may prevent UV-induced untoward skin responses via epigenetic regulation, suggesting that epigenetic modifications may provide effective therapeutic measures for the prevention and treatment of photoaging.