6-Acetonyl-5,6-dihydrosanguinarine (ADS) from Chelidonium majus L. triggers proinflammatory cytokine production via ROS–JNK/ERK–NFκB signaling pathway

Kim, Dong Hyeok; Lee, Ji Hye; Park, Soojong; Oh, Sang-Ho; Kim, Suk; Kim, Dae Wook; Park, Ki Hun; Kim, Kwang Dong

doi:10.1016/j.fct.2013.04.051

Cited by 22 publications

(8 citation statements)

References 35 publications

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“…7,[13][14][15]17,26,[51][52][53][54][55][56] To establish the level of implication of these signaling pathways in reducing the macrophage activation by nanotubular structure, the effect of selective MAPK inhibitors on the secretion of TNF-α, MCP-1, and NO was investigated. Upon treatment of RAW 264.7 cells with LPS and the ERK1/2 and p38 inhibitors, the production of all three analyzed proinflammatory mediators was significantly reduced, while the JNK inhibitor (SP600125) significantly suppressed only the TNF-α production.…”

mentioning

confidence: 99%

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

Neacsu¹,

Mazare²,

Schmuki³

et al. 2015

IJN

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Biomaterial implantation in a living tissue triggers the activation of macrophages in inflammatory events, promoting the transcription of pro-inflammatory mediator genes. The initiation of macrophage inflammatory processes is mainly regulated by signaling proteins of mitogen-activated protein kinase (MAPK) and by nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways. We have previously shown that titania nanotubes modified Ti surfaces (Ti/TiO 2 ) mitigate the immune response, compared with flat Ti surfaces; however, little is known regarding the underlying mechanism. Therefore, the aim of this study is to investigate the mechanism(s) by which this nanotopography attenuates the inflammatory activity of macrophages. Thus, we analyzed the effects of TiO 2 nanotubes on the activation of MAPK and NF-κB signaling pathways in standard and lipopolysaccharide-evoked conditions. Results showed that the Ti/TiO 2 significantly reduce the expression levels of the phosphorylated forms of p38, ERK1/2, c-Jun NH 2 -terminal kinase (JNK), IKKβ, and IkB-α. Furthermore, a significant reduction in the p65 nuclear accumulation on the nanotubular surface was remarked. Following, by using specific MAPK inhibitors, we observed that lipopolysaccharide-induced production of monocyte chemotactic protein-1 and nitric oxide was significantly inhibited on the Ti/TiO 2 surface via p38 and ERK1/2, but not via JNK. However, the selective inhibitor for JNK signaling pathway (SP600125) was effective in reducing tumor necrosis factor alpha release as well as monocyte chemotactic protein-1 and nitric oxide production. Altogether, these data suggest that titania nanotubes can attenuate the macrophage inflammatory response via suppression of MAPK and NF-κB pathways providing a potential mechanism for their anti-inflammatory activity.

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mentioning

confidence: 99%

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

Neacsu¹,

Mazare²,

Schmuki³

et al. 2015

IJN

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“…The nuclear condensation was determined by 4′,6-diamidino-2-phenylindole (DAPI) staining. HepG2 cells (5 × 10 4 cells/well) cultured in 12-well plates were incubated with and without fungal EDT for 24 h. The cells were then fixed with 3.7% ( v /v) paraformaldehyde, permeabilized with 0.1% Triton X-100 and stained with DAPI (1 mg ml −1 in PBS) [ 44 ]. After washing twice with PBS, cells were observed under fluorescence microscope (Olympus, BX51, Japan) at 10× magnification using 485 nm excitation and 535 nm emission filter sets.…”

Section: Methodsmentioning

confidence: 99%

“…The DNA fragmentation was studied as described earlier [ 44 ]. HepG2 cells were cultured in 60 mm dishes to 70% confluence prior to drug treatment.…”

Section: Methodsmentioning

confidence: 99%

Fungal 7-epi-10-deacetyltaxol produced by an endophytic Pestalotiopsis microspora induces apoptosis in human hepatocellular carcinoma cell line (HepG2)

Kamalraj

Singh

Subramani

et al. 2017

BMC Complement Altern Med

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BackgroundPaclitaxel (taxol) is a potent anticancer drug that is used in the treatment of a wide variety of cancerous. In the present study, we identified a taxol derivative named 7-epi-10-deacetyltaxol (EDT) from the culture of an endophytic fungus Pestalotiopsis microspora isolated from the bark of Taxodium mucronatum. This study was carried out to investigate the effects of fungal EDT on cell proliferation, the induction of apoptosis and the molecular mechanisms of apoptosis in human hepatoma HepG2 cells in vitro.MethodsThe endophytic fungus was identified by traditional and molecular taxonomical characterization and the fungal EDT was purified using column chromatography and confirmed by various spectroscopic and chromatographic comparisons with authentic paclitaxel. We studied the in vitro effects of EDT on HepG2 cells for parameters such as cell cycle distribution, DNA fragmentation, reactive oxygen species (ROS) generation and nuclear morphology. Further, western blot analysis was used to evaluate Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), p38-mitogen activated protein kinase (MAPK) and poly [ADP-ribose] polymerase (PARP) expression.ResultsWe demonstrate that the fungal EDT exhibited significant in vitro cytotoxicity in HepG2 cells. We investigated cytotoxicity mechanism of EDT in HepG2 cells. The results showed nuclear condensation and DNA fragmentation were observed in cells treated with fungal EDT. Besides, the fungal EDT arrested HepG2 cells at G2/M phase of cell cycle. Furthermore, fungal EDT induced apoptosis in HepG2 cells in a dose-dependent manner associated with ROS generation and increased Bax/Bcl-2 ratio, p38 MAPKs and PARP cleavage.ConclusionsOur data show that EDT induced apoptotic cell death in HepG2 cells occurs through intrinsic pathway by generation of ROS mediated and activation of MAPK pathway. This is the first report for 7-epi-10-deacetyltaxol (EDT) isolated from a microbial source.

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“…By targeting these antiviral cytokines, myriad natural small molecules have been screened. For instances, quercetin, baicalein and sanguinarine, strengthen antiviral ability, which followed by cytokines regulation (Johari et al, 2012;Kim et al, 2013;Nair et al, 2002). However, the coherent relationships between antiviral ability and up-regulating cytokines haven't been identified.…”

Section: Introductionmentioning

confidence: 92%

Anti-influenza virus effects of crude phenylethanoid glycosides isolated from ligustrum purpurascens via inducing endogenous interferon-γ

Shao

Song

et al. 2016

Journal of Ethnopharmacology

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6-Acetonyl-5,6-dihydrosanguinarine (ADS) from Chelidonium majus L. triggers proinflammatory cytokine production via ROS–JNK/ERK–NFκB signaling pathway

Cited by 22 publications

References 35 publications

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

Fungal 7-epi-10-deacetyltaxol produced by an endophytic Pestalotiopsis microspora induces apoptosis in human hepatocellular carcinoma cell line (HepG2)

Anti-influenza virus effects of crude phenylethanoid glycosides isolated from ligustrum purpurascens via inducing endogenous interferon-γ

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6-Acetonyl-5,6-dihydrosanguinarine (ADS) from Chelidonium majus L. triggers proinflammatory cytokine production via ROS–JNK/ERK–NFκB signaling pathway

Cited by 22 publications

References 35 publications

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-&kappa;B pathways

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-&kappa;B pathways

Fungal 7-epi-10-deacetyltaxol produced by an endophytic Pestalotiopsis microspora induces apoptosis in human hepatocellular carcinoma cell line (HepG2)

Anti-influenza virus effects of crude phenylethanoid glycosides isolated from ligustrum purpurascens via inducing endogenous interferon-γ

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Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways