6-Oxy-(acetyl piperazine) fluorescein as a new fluorescent labeling reagent for free fatty acids in serum using high-performance liquid chromatography

Development of a novel fluorescent tag O-2-[aminoethyl]fluorescein for the electrophoretic separation of oligosaccharides

“…by labeling with fatty acids. The outcomes of the research showed that the fluorescence of the labelled fatty acids was higher than of the derivatisation agent itself which is in contrast with the current study [25]. Even though the labelled heptose was found to be less fluorescent it was still suitable for LIF detection at 488 nm.…”

Simultaneous determination of phytohormones containing carboxyl in crude extracts of fruit samples based on chemical derivatization by capillary electrophoresis with laser-induced fluorescence detection

“…Although the derivitization chemistry of APF with fatty acids has been investigated in our previous work [25], the derivitization conditions of APF with the seven phytohormones have been optimized in order to achieve the best derivatization yield.…”

“…6-Oxy-(acetyl piperazine) fluorescein (APF) is a new derivatizing reagent for carboxyl compounds synthesized in our group [25]. As a fluorescein-based label, it has high fluorescence quantum yield (0.41 in pH9.0 Na 2 HPO 4 -H 3 Cit 3 buffer) and suitable wavelength ( ex/ em = 467/512 nm) compatible with the argon ion LIF detector.…”

The encapsulation of an amphiphile into polystyrene microspheres of narrow size distribution

“…Fluorescein has been of great interest to the scientific community due to its interesting chemical properties, its high quantum yield, availability and low costs, as well as its lack of toxicity and approval for in vivo clinical applications [12-14]. Fluorescein is an organic fluorescent molecule with molecular weight 332.3 Da, consisting of 3,6-Dihydroxyxanthane, of which the hydroxyl groups give the molecule lipophobic character and two hydrogen bond (H-bond) donors, and phthalolactone, which despite the H-bond acceptors, is largely hydrophobic (structure shown in Figure 2).…”