Simultaneous determination of phytohormones containing carboxyl in crude extracts of fruit samples based on chemical derivatization by capillary electrophoresis with laser-induced fluorescence detection

Chen, Hao; Guo, Xiao‐Feng; Zhang, Hua‐Shan; Wang, Hong

doi:10.1016/j.jchromb.2011.05.002

Cited by 59 publications

(38 citation statements)

References 28 publications

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“…Many technologies can be used for determining phytohormones, such as capillary gas chromatographic (GC), 10 capillary electrophoresis (CE), 11 radioimmunoassay, 12 high performance liquid chromatography (HPLC), 13 uorescence spectrometry 14,15 and enzyme linked immunosorbent assay (ELISA), etc. However, these methods oen require expensive apparatus or lengthy proceeding.…”

Section: Introductionmentioning

confidence: 99%

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid

Wang

et al. 2020

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid

Wang

et al. 2020

RSC Adv.

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“…Besides MS detection, ECL and LIF detections have also been reported as detection methods for CE separation . However, chemical derivatization with a proper derivatizing reagent is always indispensable for those detections to achieve the high sensitivity.…”

Section: Analytical Techniquesmentioning

confidence: 99%

“…It is noticed that GAs has a very rich fragmentation patterns, which implied that MS/MS can be used for structure elucidation of GAs. Besides MS detection, ECL and LIF detections have also been reported as detection methods for CE separation [34,58]. However, chemical derivatization with a proper derivatizing reagent is always indispensable for those detections to achieve the high sensitivity.…”

Section: Detectionsmentioning

confidence: 99%

Progress and development of analytical methods for gibberellins

Pan

Tan

Yong

et al. 2016

J of Separation Science

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Gibberellins, as a group of phytohormones, exhibit a wide variety of bio-functions within plant growth and development, which have been used to increase crop yields. Many analytical procedures, therefore, have been developed for the determination of the types and levels of endogenous and exogenous gibberellins. As plant tissues contain gibberellins in trace amounts (usually at the level of nanogram per gram fresh weight or even lower), the sample pre-treatment steps (extraction, pre-concentration, and purification) for gibberellins are reviewed in details. The primary focus of this comprehensive review is on the various analytical methods designed to meet the requirements for gibberellins analyses in complex matrices with particular emphasis on high-throughput analytical methods, such as gas chromatography, liquid chromatography, and capillary electrophoresis, mostly combined with mass spectrometry. The advantages and drawbacks of the each described analytical method are discussed. The overall aim of this review is to provide a comprehensive and critical view on the different analytical methods nowadays employed to analyze gibberellins in complex sample matrices and their foreseeable trends.

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“…They can influence and regulate organism growth, metabolism, cell division and differentiation, leaf morphologies, and senescence . Up to date, several methods have been reported for the detection of phytohormones, including spectroscopy , CE , CE–LIF , impedance immunosensor , GC–MS , HPLC , nano‐HPLC–MS/MS , HPLC–MS/MS , and UHPLC–MS/MS . Among them, spectroscopic methodology has the lowest accuracy.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of ten phytohormones in Sargassum horneri by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

Zhou

Yan

et al. 2016

J of Separation Science

107

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Phytohormones have attracted wide attention due to their important biological functions. However, their detection is still a challenge because of their complex composition, low abundance and diverse sources. In this study, a novel method of high-performance liquid chromatography with electrospray ionization tandem mass spectrometry was developed and validated for the simultaneous determination of ten phytohormones including indole-3-acetic acid, isopentenyladenine, isopentenyl adenosine, trans-zeatin riboside, zeatin, strigolactones, abscisic acid, salicylic acid, gibberellin A3, and jasmonic acid in Sargassum horneri (S. horneri). The phytohormones were extracted from freeze-dried S. horneri with methanol/water/methanoic acid (15:4:1, v/v/v) analyzed on a Hypersil Gold C18 column and detected by electrospray ionization tandem triple quadrupole mass spectrometry in the multiple reaction monitoring mode. The experimental conditions for the extraction and analysis of phytohormones were optimized and validated in terms of reproducibility, linearity, sensitivity, recovery, accuracy, and stability. Distributions of the phytohormones in the stems, blades, and gas bladder of the S. horneri in drift, fixed, and semi-fixed growing states were investigated for the first time. The observed contents of the phytohormones in S. horneri range from not detected to 5066.67 ng/g (fresh weight). Most phytohormones are distributed mainly in the stems of S. horneri in drift and semi-fixed states.

show abstract

Simultaneous determination of phytohormones containing carboxyl in crude extracts of fruit samples based on chemical derivatization by capillary electrophoresis with laser-induced fluorescence detection

Cited by 59 publications

References 28 publications

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid

Progress and development of analytical methods for gibberellins

Simultaneous analysis of ten phytohormones in Sargassum horneri by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

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