[60]Fullerene for Medicinal Purposes, A Purity Criterion towards Regulatory Considerations

Keykhosravi, Sanaz; Rietveld, Ivo B.; Couto, Diana; Tamarit, J. Ll.; Céolin, R.; Moussa, Fathi

doi:10.3390/ma12162571

Cited by 10 publications

(6 citation statements)

References 39 publications

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“…The method is based on transferring C 60 fullerenes from organic solution into the aqueous phase by ultrasonic treatment. The purity of the prepared C 60 FAS (i.e., the presence/absence of any residual impurities such as carbon black and toluene phase) was determined by high-performance liquid chromatography (HPLC) and GC/MS analysis (Keykhosravi et al, 2019). The state of C 60 fullerene in aqueous solution was monitored using atomic force microscopy (AFM "Solver Pro M" system, NT-MDT, Russia) as well as the dynamic light scattering (DLS) method (Zetasizer Nano-ZS90, Malvern, Worcestershire, UK) at room temperature.…”

Section: Materials Preparation and Characterizationmentioning

confidence: 99%

C60 fullerenes increase the intensity of rotational movements in non-anesthetized hemiparkinsonic rats

Maznychenko¹,

Mankivska²,

Верещака³

et al. 2020

Acta Neurobiologiae Experimentalis

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The effect of C60 fullerene aqueous colloid solution (C60FAS) on the intensity of long-lasting (persisting for one hour) rotational movements in non-anesthetized rats was investigated. For this purpose, an experimental hemiparkinsonic animal model was used in the study. Rotational movements in hemiparkinsonic animals were initiated by the intraperitoneal administration of the dopamine receptor agonist apomorphine. It was shown that a preliminary injection of C60FAS (a substance with powerful antioxidant properties) in hemiparkinsonic rats induced distinct changes in animal motor behavior. It was revealed that fullerene-pretreated animals, in comparison with non-pretreated or vehicle-pretreated rats, rotated for 1 h at an approximately identical speed until the end of the experiment, whereas the rotation speed of control rats gradually decreased to 20-30% of the initial value. One can assume that the observed changes in the movement dynamics of the hemiparkinsonic rats after C60FAS pretreatment presumably can be induced by the influence of C60FAS on the dopaminergic system, although the isolated potentiation of the action of apomorphine C60FAS cannot be excluded. Nevertheless, earlier data on the action of C60FAS on muscle dynamics has suggested that C60FAS can activate a protective action of the antioxidant system in response to long-lasting muscular activity and that the antioxidant system in turn may directly decrease fatigue-related changes during long-lasting muscular activity.

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Section: Materials Preparation and Characterizationmentioning

confidence: 99%

C60 fullerenes increase the intensity of rotational movements in non-anesthetized hemiparkinsonic rats

Maznychenko¹,

Mankivska²,

Верещака³

et al. 2020

Acta Neurobiologiae Experimentalis

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“… 13 Therefore, C60 is informally sold as a “health food” ingredient in the United States and the European Union. 14 …”

Section: Introductionmentioning

confidence: 99%

“…13 Therefore, C60 is informally sold as a "health food" ingredient in the United States and the European Union. 14 C60 dissolved in olive oil functions as a very strong antioxidant and has a therapeutic effect on inflammatory skin diseases, cancers, and intestinal diseases. [11][12][13][14][15][16][17][18] The authors of this study discovered that cosmetic-grade C60 dissolved in grape seed oil could quickly and noticeably relieve skin itching and pain in muscles, tendons, and joints when applied and massaged on the skin (patent applied for 19 ).…”

Section: Introductionmentioning

confidence: 99%

“…14 C60 dissolved in olive oil functions as a very strong antioxidant and has a therapeutic effect on inflammatory skin diseases, cancers, and intestinal diseases. [11][12][13][14][15][16][17][18] The authors of this study discovered that cosmetic-grade C60 dissolved in grape seed oil could quickly and noticeably relieve skin itching and pain in muscles, tendons, and joints when applied and massaged on the skin (patent applied for 19 ). In this study, C60 dissolved in grape seed oil (3 mg/mL) was used as the research object, and its anti-inflammatory and antioxidant effects were investigated at the cellular, molecular, and wholeanimal levels.…”

Section: Introductionmentioning

confidence: 99%

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Anti-Inflammatory and Antioxidant Effects of Liposoluble C60 at the Cellular, Molecular, and Whole-Animal Levels

Hui¹,

Jia²,

Li³

et al. 2023

JIR

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Introduction Liposoluble carbon-60 (C60) has potential applications in many fields, including cosmetics, medical devices, and medicine, but its specific mechanism of action remains unclear. This study explored whether liposoluble C60 could be delivered to human organs, tissues, and cells through blood, extracellular fluid, and cell culture fluid and whether it exerts anti-inflammatory and antioxidant effects at the molecular, cellular, and whole-animal levels. Methods At the cellular level, we mixed C60 dissolved in grape seed oil with cell culture medium containing 10% serum and investigated its effects on tumor necrosis factor-α (TNF-α) release, migration, phagocytosis, respiratory burst, and apoptosis in freshly isolated human neutrophils. At the molecular level, we mixed a trace amount of C60 dissolved in grape seed oil with aqueous and ethanolic solutions and studied its antioxidant effect. At the animal level, we investigated the inhibitory effect of C60 on the serum inflammatory marker C-reactive protein (CRP) in beagle dogs after oral administration of C60 dissolved in grape seed oil. Results The results showed that the trace amount of C60 dissolved in grape seed oil significantly inhibited TNF-α release, cell migration, phagocytosis, and respiratory burst in freshly isolated human neutrophils. In addition, the trace amount of C60 dissolved in grape seed oil had a significant scavenging effect on superoxide free radicals and 1,1-diphenyl-2-trinitrophenylhydrazine free radicals. Oral administration of C60 dissolved in grape seed oil markedly reduced the level of the serum inflammatory marker CRP in beagle dogs. Conclusion In summary, a trace amount of hydrophobic C60 in hydrophilic media effectively produced anti-inflammatory and antioxidant effects in cells and animals. C60 dissolved in grape seed oil is a novel anti-inflammatory and antioxidant drug candidate.

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“…Extensive research into the use of carbon materials in biological and biomedical studies has been carried out for several years. These materials include carbon nanotubes, 1,2 fullerene, 3 graphene, graphene derivatives, particularly graphene oxide (GO) and reduced GO. [4][5][6][7] GO is currently being tested in engineered tissue, [8][9][10] implants, 11 diagnostic tools and chips, enhancing biological images, 12 drug-delivery carriers, 13 bone regeneration, 14 and antibacterial materials.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide as a Collagen Modifier of Amniotic Membrane and Burnt Skin

Pielesz¹,

Ślusarczyk²,

Sieradzka³

et al. 2021

NSA

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Introduction:The aim of this interdisciplinary study was to answer the question of whether active antioxidants as graphene oxide (GO), sodium ascorbate, and L-ascorbic acid modify at a molecular and supramolecular level the tissue of pathological amnion and the necrotic eschar degraded in thermal burn. We propose new solutions of modifiers based on GO that will become innovative ingredients to be used in transplants (amnion) and enhance regeneration of epidermis degraded in thermal burn. Methods: A Nicolet 6700 spectrophotometer with Omnic software and the EasiDiff diffusion accessory were used in FTIR spectroscopic analysis. A Nicolet Magna-IR 860 spectrometer with an FT Raman accessory was used to record the Raman spectra of the samples. The surface of the samples was examined using a Phenom ProX scanning electron microscope with an energy-dispersive X-ray spectroscopy detector to diagnose and illustrate morphological effects on skin and amnion samples. SAXS measurements were carried out with a compact Kratky camera equipped with the SWAXS optical system. Results: Characterisation of amide I-III regions, important for molecular structure, on both FTIR and FTR spectra revealed distinct shifts, testifying to organization of protein structure after GO modification. A wide lipid band associated with ester-group vibrations in phospholipids of cell membranes and vibrations of the carbonyl group of GO in the 1,790-1,720 cm −1 band were observed in the spectra of thermally degraded and GO-modified epidermis and pathological amnion. SAXS studies revealed that GO caused a significant change in the structure of the burnt skin, but its influence on the structure of the amnion was weak. Conclusion: Modification of burn-damaged epidermis and pathological amnion by means of GO results in stabilization and regeneration of tissue at the level of molecular (FTIR, FTR) and supramolecular (SAXS) interactions.

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[60]Fullerene for Medicinal Purposes, A Purity Criterion towards Regulatory Considerations

Cited by 10 publications

References 39 publications

C60 fullerenes increase the intensity of rotational movements in non-anesthetized hemiparkinsonic rats

C60 fullerenes increase the intensity of rotational movements in non-anesthetized hemiparkinsonic rats

Anti-Inflammatory and Antioxidant Effects of Liposoluble C60 at the Cellular, Molecular, and Whole-Animal Levels

Graphene Oxide as a Collagen Modifier of Amniotic Membrane and Burnt Skin

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