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Abstract

Mutations in the myosin XVA gene (MYO15A) cause congenital non-syndromic deafness in humans and mice. Therefore, the M YO15A gene represents a candidate gene for hereditary hearing loss in dogs. Using a human cDNA to screen a dog BAC library, we isolated a canine BAC clone. Sequencing of the BAC ends confirmed homology to the human gene. To facilitate future linkage studies, we report the physical mapping of the canine MYO15A gene to CFA5q23-q24 by FISH and RH mapping.

A genome-wide association study of deafness in three canine breeds

“…In addition to microarray GWAS studies, sequencing studies of several candidate genes in deaf vs. hearing dogs have been performed, resulting in reported elimination of the genes EDNRB and KIT [45], MYO15A [46], PAX3 [47], TMC1 and TMIE [48], SILV [49], and ESPN, MYO3A, SLC26A5, and USH1C [50]. However, not all of the listed genes have been eliminated in all three breeds of this study.…”

STRING data mining of GWAS data in canine hereditary pigment-associated deafness

“…Genome wide association studies (GWAS) and other molecular genetic studies of deafness by our lab and others have failed to identify loci significantly associated with deafness ( Cargill, 2004 ; Kluth and Distl, 2013 ; Stritzel et al., 2009 ; Sommerlad et al., 2010 ; Hayward et al, 2020 ), a common outcome with complex disorders ( Altshuler et al., 2008 ; Rudy, 2012 ). Numerous specific candidate genes have been ruled out: EDNRB and KIT ( Metallinos and Rine, 2000 ), MYO15A ( Rak et al., 2002 ), PAX3 ( Brenig et al., 2003 ), TMC1 and TMIE ( Mieskes and Distl, 2006 ), SILV ( Stritzel et al., 2007 ), and ESPN, MYO3A, SLC26A5 , and USH1C ( Mieskes and Distl, 2007 ). These unsuccessful outcomes indicate the need for different bioinformatics data mining approaches.…”