639 Regulatory function of TRPV4 on the dermal component of inflammatory skin conditions

Abstract

Diabetes Mellitus (DM) can result in chronic non-healing wounds ultimately leading to lower limb amputations. Studies have shown haired punch grafts can accelerate wound closure when compared with non-haired grafts, however the contribution of different populations of fibroblasts from haired skin to wound healing under elevated glucose levels and the effects of high glucose concentrations (as seen in DM) on these cells are unknown. We aimed to determine the effect of basal (1g/L) and high glucose (4.5g/L) concentrations on three different fibroblast subpopulations cultured from haired skin (papillary dermis (DF), hair follicle dermal sheath (DS) and hair follicle dermal papilla (DP)). Cell counts showed proliferation of all cell subtypes decreased in high glucose compared to basal levels in the presence of 10% serum. Ki-67 positive cells were also reduced in high glucose treated cells compared to basal. The