7-Hydroxy Frullanolide, a sesquiterpene lactone, increases intracellular calcium amounts, lowers CD4+ T cell and macrophage responses, and ameliorates DSS-induced colitis

Pathak, Sanmoy; Gokhroo, Abhijeet; Dubey, Ashim Kumar; Majumdar, Shamik; Gupta, Souradeep; Almeida, Asha; Mahajan, Girish; Kate, Abhijeet S.; Prabhu, K. Narayan; Sharma, Rajiv; Kumar, Sanjay; Vishwakarma, Ram A.; Balakrishnan, Arun; Atreya, Hanudatta S.; Nandi, Dipankar

doi:10.1016/j.intimp.2021.107655

Cited by 10 publications

(17 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, the effects of 7-hydroxyfrullanoide in inhibiting CD4 + T cells and peritoneal macrophage responses were investigated. The 7-hydroxyfrullanoide reduced IL-2 and simultaneously induced Ca 2+ (an intracellular chelator, which lowers lactate and rescues CD4 + T cell cycling) [ 46 ]. Moreover, intraperitoneal administration of 7-hydroxyfrullanoide lowers serum inflammatory cytokines IFN- γ, IL-6, reduces the effects of dextran sulfate sodium-induced colitis, and emphasizes the anti-inflammatory potential of 7-hydroxyfrullanoide in lowering immune responses [ 46 ].…”

Section: Sesquiterpene Lactones In Medicine: Immunoregulatory Respons...mentioning

confidence: 99%

“…The 7-hydroxyfrullanoide reduced IL-2 and simultaneously induced Ca 2+ (an intracellular chelator, which lowers lactate and rescues CD4 + T cell cycling) [46]. Moreover, intraperitoneal administration of 7-hydroxyfrullanoide lowers serum inflammatory cytokines IFN-γ, IL-6, reduces the effects of dextran sulfate sodium-induced colitis, and emphasizes the anti-inflammatory potential of 7-hydroxyfrullanoide in lowering immune responses [46]. Abe et al (2015) reports a role of the SL tagitinins isolated of Tithonia diversifolia in activation and survival of human neutrophils [47].…”

Section: Immunomodulatory Effects Of Sesquiterpene Lactones At the Ce...mentioning

confidence: 99%

See 1 more Smart Citation

Anti-Inflammatory and Immunoregulatory Action of Sesquiterpene Lactones

et al. 2022

View full text Add to dashboard Cite

Sesquiterpene lactones (SL), characterized by their high prevalence in the Asteraceae family, are one of the major groups of secondary metabolites found in plants. Researchers from distinct research fields, including pharmacology, medicine, and agriculture, are interested in their biological potential. With new SL discovered in the last years, new biological activities have been tested, different action mechanisms (synergistic and/or antagonistic effects), as well as molecular structure–activity relationships described. The review identifies the main sesquiterpene lactones with interconnections between immune responses and anti-inflammatory actions, within different cellular models as well in in vivo studies. Bioaccessibility and bioavailability, as well as molecular structure–activity relationships are addressed. Additionally, plant metabolic engineering, and the impact of sesquiterpene lactone extraction methodologies are presented, with the perspective of biological activity enhancement. Sesquiterpene lactones derivatives are also addressed. This review summarizes the current knowledge regarding the therapeutic potential of sesquiterpene lactones within immune and inflammatory activities, highlighting trends and opportunities for their pharmaceutical/clinical use.

show abstract

Section: Sesquiterpene Lactones In Medicine: Immunoregulatory Respons...mentioning

confidence: 99%

Section: Immunomodulatory Effects Of Sesquiterpene Lactones At the Ce...mentioning

confidence: 99%

Anti-Inflammatory and Immunoregulatory Action of Sesquiterpene Lactones

et al. 2022

View full text Add to dashboard Cite

show abstract

“…168 On the contrary, other studies have reported that sesquiterpene lactones, such as 7-hydroxy frullanolide, inhibit both CD4 + T cell activation and peritoneal macrophage responses by opening up plasma membrane Ca 2+ channels to increase intracellular Ca 2+ levels. 169 Parthenolide can reduce NF-κB signaling by preventing its binding to DNA and enhancing IκB-kinase activity. 170 The sesquiterpene lactone fraction extracts from Artemissia khorassanica in vitro can inhibit the production of NO and PGE 2 by downregulating COX and iNOS expression in macrophages.…”

Section: Terpenes and Their Derivativesmentioning

confidence: 99%

Plant-based Immunomodulators and Their Potential Therapeutic Actions

Pathak¹,

Fialho²,

Nandi³

2022

J Explor Res Pharmacol

Self Cite

View full text Add to dashboard Cite

Immunomodulation is a diverse process by which immunomodulators enhance or suppress immune responses to control disease progression. Immunomodulators are a broad class of drugs that include immunosuppressants and immunostimulants. These agents have been used to fight against the dysregulated immune responses observed during tissue/organ transplantation and disorders, such as rheumatoid arthritis, ulcerative colitis, and cancers. Immunomodulators obtained from a myriad of plant sources are a major class of compounds that are known to have medicinal properties and are used for the treatment of various diseases. However, the mechanisms underlying the action of plant-derived compounds are poorly understood. Here, we discuss the major classes of plantbased immunomodulators with examples and their effects on the major signaling pathways, such as the nuclear factor kappa light chain enhancer of activated B cells (NF-κB), mitogen-activated protein kinase (MAPK), and mammalian target of rapamycin pathways. Importantly, we discuss the preclinical and clinical research to date to understand the importance of these immunomodulators. Overall, this review highlights the significance of plantbased immunomodulators as an alternative therapeutic strategy for combating various diseases.

show abstract

“…The cell-mediated immune responses that T cells orchestrate are critical in fighting infections caused by intracellular pathogens, including bacteria and viruses, and cancer. Optimal activation of T cells is vital to elicit a protective immune response. − Aberrant activation of T cells causes autoimmune diseases, , whereas suboptimal activation can result in an immunocompromised condition. T cells originate in the bone marrow, mature in the thymus, and migrate to secondary lymphoid organs, namely the spleen and lymph nodes, where they remain naïve until activated .…”

mentioning

confidence: 99%

“…A T cell line or primary T cells isolated from mice can serve as useful models to make a lymph node-mimetic construct ex vivo . Primary T cells, although viable for a short time in vitro in the absence of exogenous stimuli, produce functional responses that are physiologically relevant to the in vivo system and are, therefore, useful in investigating T cell biology in normal and diseased conditions. , In this study, EL4 cells (a mouse T cell lymphoma line) and primary mouse T cells were 3D bioprinted in GelMA to generate cell-laden hydrogels toward engineering an ex vivo lymph node-mimetic tissue microenvironment. The 3D printing parameters were tuned to vary the hydrogel stiffness to closely mimic the biophysical microenvironment of soft tissues.…”

mentioning

confidence: 99%

Mechanical Properties Affect Primary T Cell Activation in 3D Bioprinted Hydrogels

et al. 2023

Self Cite

View full text Add to dashboard Cite

T cells play a critical role in the adaptive immune response of the body, especially against intracellular pathogens and cancer. In vitro, T cell activation studies typically employ planar (two-dimensional, 2D) culture systems that do not mimic native cell-to-extracellular matrix (ECM) interactions, which influence activation. The goal of this work was to study T cell responses in a cell line (EL4) and primary mouse T cells in three-dimensional (3D) bioprinted matrices of varied stiffness. Cell-laden hydrogels were 3D bioprinted from gelatin methacryloyl (GelMA) using a digital light processing (DLP)-based 3D bioprinter operated with visible light (405 nm). Mechanical characterization revealed that the hydrogels had pathophysiologically relevant stiffnesses for a lymph node-mimetic tissue construct. EL4, a mouse T cell lymphoma line, or primary mouse T cells were 3D bioprinted and activated using a combination of 10 ng/mL of phorbol myristate acetate (PMA) and 0.1 μM of ionomycin. Cellular responses revealed differences between 2D and 3D cultures and that the biomechanical properties of the 3D bioprinted hydrogel influence T cell activation. Cellular responses of the 2D and 3D cultures in a soft matrix (19.83 ± 2.36 kPa) were comparable; however, they differed in a stiff matrix (52.95 ± 1.36 kPa). The fraction of viable EL4 cells was 1.3-fold higher in the soft matrix than in the stiff matrix. Furthermore, primary mouse T cells activated with PMA and ionomycin showed 1.35-fold higher viable cells in the soft matrix than in the stiff matrix. T cells bioprinted in a soft matrix and a stiff matrix released 7.4-fold and 5.9-fold higher amounts of interleukin-2 (IL-2) than 2D cultured cells, respectively. Overall, the study demonstrates the changes in the response of T cells in 3D bioprinted scaffolds toward engineering an ex vivo lymphoid tissue-mimetic system that can faithfully recapitulate T cell activation and unravel pathophysiological characteristics of T cells in infectious biology, autoimmunity, and cancers.

show abstract

7-Hydroxy Frullanolide, a sesquiterpene lactone, increases intracellular calcium amounts, lowers CD4+ T cell and macrophage responses, and ameliorates DSS-induced colitis

Cited by 10 publications

References 51 publications

Anti-Inflammatory and Immunoregulatory Action of Sesquiterpene Lactones

Anti-Inflammatory and Immunoregulatory Action of Sesquiterpene Lactones

Plant-based Immunomodulators and Their Potential Therapeutic Actions

Mechanical Properties Affect Primary T Cell Activation in 3D Bioprinted Hydrogels

Contact Info

Product

Resources

About