703 Human recombinant IgE-dependent histamine releasing factor (HrHRF) enhances IL-4 secretion by human basophils

Schroeder, John T.; Lichtenstein, L.M.; Kagey-Sobotka, A.; MacDonald, Susan

doi:10.1016/s0091-6749(96)80921-3

Cited by 41 publications

(56 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, HRF could increase histamine and IL-4 from IgE Ϫ basophils when preincubated with HRF and challenged with anti-IgE Ab (18). Also, HRF did not bind to IgE.…”

Section: Discussionmentioning

confidence: 91%

“…PMB abolished the effects of LPS on Ig production dramatically, except for IgM, but it had no effect on those of rHRF. HRF enhanced IL-4 and IL-13 secretion by human basophils (18), and it was observed that HRF induced IL-6 and IL-10 gene expression in B cells (see below). Its effects on Ig production may be due to the induced cytokines.…”

Section: Effects Of Hrf On the Proliferation And Ig Production Of B Cmentioning

confidence: 94%

“…Subsequent studies have been shown that IgE-HRF plays an important role in perpetuating late phase allergic reaction (15,16). In addition, IgE-HRF stimulates the production of IL-4 and IL-13 from all basophils in the presence of IgE (17,18). However, additional data suggest that IgE-HRF has a unique signaling pathway and binds to a specific receptor other than IgE (19).…”

Section: A Ctivation Of B Cells During the Different Stages Of Immunementioning

confidence: 99%

See 2 more Smart Citations

Molecular Identification of IgE-Dependent Histamine-Releasing Factor as a B Cell Growth Factor

Kang

Lee

Song

et al. 2001

The Journal of Immunology

View full text Add to dashboard Cite

The culture supernatants of LK1 cells, murine erythroleukemia cells, showed B cell-stimulating activity. Purification and NH2-terminal sequence analysis revealed that one of the candidates was murine IgE-dependent histamine-releasing factor (IgE-HRF), which is known to induce histamine from basophils. Recombinant IgE-HRF (rHRF) obtained from Escherichia coli- or 293-transformed embryonal kidney cells was tested for B cell-stimulating activity. Both rHRFs stimulated B cell proliferation in a dose-dependent manner. However, boiling or anti-HRF Ab abolished the B cell stimulatory effects of rHRF. Recombinant HRF showed strong synergistic effects with IL-2, IL-4, and IL-5 for B cell activation, with maximal activity in the presence of anti-CD40 Ab. Recombinant HRF increased MHC class II expression of B cells. It also increased Ig production from B cells. Treatment with polymyxin B, a neutralizing peptide antibiotic of LPS, did not reduce the activity of rHRF. In addition, FACS analysis using PE-conjugated rHRF showed that HRF bound to B cells. Recombinant HRF up-regulated the expression of IL-1 and IL-6 in B cells. In vivo administration of rHRF or the cDNA for rHRF increased total and Ag-specific Ig synthesis. Taken together, these results indicate that HRF stimulates B cell activation and function.

show abstract

“…However, HRF could increase histamine and IL-4 from IgE Ϫ basophils when preincubated with HRF and challenged with anti-IgE Ab (18). Also, HRF did not bind to IgE.…”

Section: Discussionmentioning

confidence: 91%

Section: Effects Of Hrf On the Proliferation And Ig Production Of B Cmentioning

confidence: 94%

Section: A Ctivation Of B Cells During the Different Stages Of Immunementioning

confidence: 99%

See 1 more Smart Citation

Molecular Identification of IgE-Dependent Histamine-Releasing Factor as a B Cell Growth Factor

Kang

Lee

Song

et al. 2001

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Subsequently, TPT1/TCTP was characterized as the histamine releasing factor (HRF) and studied for its extrinsic function in allergy and inflammation [4][5][6][7].…”

Section: Initial Discoveriesmentioning

confidence: 99%

TPT1/ TCTP-regulated pathways in phenotypic reprogramming

Amson

Pece

Marine

et al. 2013

Trends in Cell Biology

106

112

View full text Add to dashboard Cite

“…HrHRF has been shown to prime human basophils for enhanced histamine release after anti-IgE stimulation as well as to directly induce degranulation in a subset of donors (6,7). Therefore, we first tested the ability of HrHRF (8 g/ml GST-tagged HrHRF) to directly induce the secretion of the cytokines IL-2, IL-13, and IFN-␥ after a 24-h incubation.…”

Section: Preincubation With Hrhrf Inhibits Primary T Cell Cytokine Prmentioning

confidence: 99%

Inhibition of Cytokine Gene Transcription by the Human Recombinant Histamine-Releasing Factor in Human T Lymphocytes

Vonakis¹,

Sora²,

Langdon³

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

Human recombinant histamine-releasing factor (HrHRF) preincubation enhances the secretion of histamine, IL-4, and IL-13 from FcεRI-stimulated human basophils. In GM-CSF-primed human eosinophils, HrHRF increases IL-8 production. Our recent experiments were designed to evaluate the effects of HrHRF on human T cell cytokine production. Purified T cells were preincubated with GST-tagged HrHRF, followed by stimulation with PMA and A23187 overnight. A partial inhibition of IL-2 and IL-13 production (30 and 75%, respectively) was detected compared with that in cells treated with PMA/A23187 alone. However, the production of IFN-γ was similar in PMA/A23187 stimulated cells with or without HrHRF. The inhibition of cytokine protein production was dose dependent and specific to the HrHRF portion of GST-HrHRF. The inhibition was not due to endotoxin, since preincubation with polymyxin B and HrHRF gave similar results to that with HrHRF alone. The same pattern and specificity of cytokine regulation were replicated in the Jurkat T cell line as for primary T cells. The PMA/A23187-stimulated activity of a proximal promoter IL-13, IL-4, or IL-2 luciferase construct transfected into Jurkat cells was partially inhibited (60, 32, or 70%, respectively) upon GST-HrHRF preincubation, suggesting that HrHRF functions to inhibit cytokine production in Jurkat cells by preventing gene transcription. The inhibition of IL-2 promoter activation was specific to the HrHRF portion of GST-HrHRF. We conclude that HrHRF, in addition to functioning as a histamine-releasing factor, can differentially modulate the secretion of cytokines from human basophils, eosinophils, T cells, and murine B cells, suggesting that it may induce a complex array of responses at sites of allergic inflammation.

show abstract

703 Human recombinant IgE-dependent histamine releasing factor (HrHRF) enhances IL-4 secretion by human basophils

Cited by 41 publications

References 0 publications

Molecular Identification of IgE-Dependent Histamine-Releasing Factor as a B Cell Growth Factor

Molecular Identification of IgE-Dependent Histamine-Releasing Factor as a B Cell Growth Factor

TPT1/ TCTP-regulated pathways in phenotypic reprogramming

Inhibition of Cytokine Gene Transcription by the Human Recombinant Histamine-Releasing Factor in Human T Lymphocytes

Contact Info

Product

Resources

About