8-Hydroxy-2-Deoxyguanosine as Oxidative DNA Damage Biomarker of Medical Ionizing Radiation: A Scoping Review

AbuArrah, Muhammad; Setianto, Budi Yuli; Faisal, Arif; Sadewa, Ahmad Hamim

doi:10.31661/jbpe.v0i0.2101-1258

Cited by 17 publications

(9 citation statements)

References 24 publications

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“…The state of oxidative stress is a normal phenomenon in cellular functioning, especially during energy production. However, the overexpression of ROS, exceeding the tolerance of redox homeostasis, causes DNA damage [29], such as DNA double-strand breaks (γH2AX) [30,31] and oxidative DNA damage (8-OHdG) [32][33][34]. Consistently, StenBpromoted oxidative stress generates DNA damage (γH2AX and 8-OHdG) (Figures 8 and 9).…”

Section: Discussionmentioning

confidence: 81%

“…Two types of DNA damage were detected and assessed through antibody-based analyses [47], including markers for DNA double-strand breaks (γH2AX) [30,31] and oxidative DNA damage (8-OHdG) [32,33]. Briefly, 75% ethanol-fixed cells were processed with γH2AX primary antibody/secondary antibody/7AAD (5 µg/mL) treatments [40] or the 8-OHdG primary antibody [47] (Santa Cruz Biotechnology, Santa Cruz, CA, USA) to detect the γH2AX and 8-OHdG levels, respectively.…”

Section: Dna Damagesmentioning

confidence: 99%

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Boesenbergia stenophylla-Derived Stenophyllol B Exerts Antiproliferative and Oxidative Stress Responses in Triple-Negative Breast Cancer Cells with Few Side Effects in Normal Cells

Lee

Shiau

Tang

et al. 2023

IJMS

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Triple-negative breast cancer (TNBC) is insensitive to target therapy for non-TNBC and needs novel drug discovery. Extracts of the traditional herb Boesenbergia plant in Southern Asia exhibit anticancer effects and contain novel bioactive compounds but merely show cytotoxicity. We recently isolated a new compound from B. stenophylla, stenophyllol B (StenB), but the impact and mechanism of its proliferation-modulating function on TNBC cells remain uninvestigated. This study aimed to assess the antiproliferative responses of StenB in TNBC cells and examine the drug safety in normal cells. StenB effectively suppressed the proliferation of TNBC cells rather than normal cells in terms of an ATP assay. This preferential antiproliferative function was alleviated by pretreating inhibitors for oxidative stress (N-acetylcysteine (NAC)) and apoptosis (Z-VAD-FMK). Accordingly, the oxidative-stress-related mechanisms were further assessed. StenB caused subG1 and G2/M accumulation but reduced the G1 phase in TNBC cells, while normal cells remained unchanged between the control and StenB treatments. The apoptosis behavior of TNBC cells was suppressed by StenB, whereas that of normal cells was not suppressed according to an annexin V assay. StenB-modulated apoptosis signaling, such as for caspases 3, 8, and 9, was more significantly activated in TNBC than in normal cells. StenB also caused oxidative stress in TNBC cells but not in normal cells according to a flow cytometry assay monitoring reactive oxygen species, mitochondrial superoxide, and their membrane potential. StenB induced greater DNA damage responses (γH2AX and 8-hydroxy-2-deoxyguanosine) in TNBC than in normal cells. All these StenB responses were alleviated by NAC pretreatment. Collectively, StenB modulated oxidative stress responses, leading to the antiproliferation of TNBC cells with little cytotoxicity in normal cells.

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Section: Discussionmentioning

confidence: 81%

Section: Dna Damagesmentioning

confidence: 99%

Boesenbergia stenophylla-Derived Stenophyllol B Exerts Antiproliferative and Oxidative Stress Responses in Triple-Negative Breast Cancer Cells with Few Side Effects in Normal Cells

Lee

Shiau

Tang

et al. 2023

IJMS

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“…Two types of DNA damage (double‐strand breaks and oxidative change) were measured by the markers of γH2AX 40–42 and 8‐OHdG 28,43,44 . In flow cytometry, 75% ethanol‐fixed cells were mixed with primary antibodies (4°C, 1 h) for γH2AX and 8‐OHdG‐FITC (Santa Cruz Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

“…28 2.7 | γH2AX and 8-hydroxy-2-deoxyguanosine (8-OHdG) for DNA damage detection Two types of DNA damage (double-strand breaks and oxidative change) were measured by the markers of γH2AX [40][41][42] and 8-OHdG. 28,43,44 In flow cytometry, 75% ethanol-fixed cells were mixed with primary antibodies (4 C, 1 h) for γH2AX and 8-OHdG-FITC (Santa Cruz Biotechnology). Compared to the 8-OHdG assay, detecting γH2AX required additional steps to add a secondary antibody and 7AAD (5 μg/mL, 30 min).…”

Section: Reactive Oxygen Species (Ros) Mitochondrial Superoxide (Mito...mentioning

confidence: 99%

The neddylation inhibitor MLN4924 inhibits proliferation and triggers apoptosis of oral cancer cells but not for normal cells

Chen,

Chan,

Shiau

et al. 2023

Environmental Toxicology

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Increased neddylation benefits the survival of several types of cancer cells. The inhibition of neddylation has the potential to exert anticancer effects but is rarely assessed in oral cancer cells. This study aimed to investigate the antiproliferation potential of a neddylation inhibitor MLN4924 (pevonedistat) for oral cancer cells. MLN4924 inhibited the cell viability of oral cancer cells more than that of normal oral cells (HGF‐1) with 100% viability, that is, IC50 values of oral cancer cells (CAL 27, OC‐2, and Ca9‐22) are 1.8, 1.4, and 1.9 μM. MLN4924 caused apoptotic changes such as the subG1 accumulation, activation of annexin V, pancaspase, and caspases 3/8/9 of oral cancer cells at a greater rate than in normal oral cells. MLN4924 induced greater oxidative stress in oral cancer cells compared to normal cells by upregulating reactive oxygen species and mitochondrial superoxide and depleting the mitochondrial membrane potential and glutathione. In oral cancer cells, preferential inductions also occurred for DNA damage (γH2AX and 8‐oxo‐2′‐deoxyguanosine). Therefore, this investigation demonstrates that MLN4924 is a potential anti‐oral‐cancer agent showing preferential inhibition of apoptosis and promotion of DNA damage with fewer cytotoxic effects on normal cells.

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“…In previous studies it has been established as a sensitive biomarker for the assessment of oxidative DNA modification. 11 8-OH-dG levels also increase due to other factors such as smoking, aging, or exposure to physical, chemical, and biological substances. 12 Wide range of 8-OH-dG cut-off values was reported in different studies.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Hyperlipidemia Associated with Increased Levels of Oxidized Low-Density Lipoproteins and 8-Hydroxy-2´-Deoxyguanosine

Babakr¹,

Mukhtar²,

Althubiti³

et al. 2023

DMSO

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Background Hyperlipidemia is a common risk factor for atherosclerosis, heart diseases, and other pathological conditions. The factors leading to the oxidation of native low-density lipoproteins remain of valuable importance for a better understanding of the mechanisms leading to these pathologies. The aim of the present study was to evaluate the association between lipid status and the levels of oxidized low-density lipoproteins and 8-hydroxy-2´-deoxyguanosine. Methods One hundred and fourteen participants were enrolled. Lipid profile parameters were measured and used individually to categorize subjects into two groups of normal and hyperlipidemic cases according to the international reference ranges. Oxidized low-density lipoproteins and 8-hydroxy-2´-deoxyguanosine were then compared in normal and high lipid profile groups. The obtained results were then statistically analyzed. Results 8-Hydroxy-2´-deoxyguanosine was found to be positively correlated with hypercholesterolemia, hypertriglyceridemia, and high levels of low-density lipoproteins (r = 0.53, 0.41, and 0.60), respectively ( p <0.001). A positive correlation was observed also between the levels of oxidized low-density lipoproteins and the same lipid profile parameters (r = 0.42, 0.31, and 0.45), respectively ( p <0.001). Conclusion The present study suggests that disturbance in lipid profile may result in increased levels of oxidized low-density lipoproteins and oxidative stress in the study group; however, a larger sample is needed to confirm the present findings.

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8-Hydroxy-2-Deoxyguanosine as Oxidative DNA Damage Biomarker of Medical Ionizing Radiation: A Scoping Review

Cited by 17 publications

References 24 publications

Boesenbergia stenophylla-Derived Stenophyllol B Exerts Antiproliferative and Oxidative Stress Responses in Triple-Negative Breast Cancer Cells with Few Side Effects in Normal Cells

Boesenbergia stenophylla-Derived Stenophyllol B Exerts Antiproliferative and Oxidative Stress Responses in Triple-Negative Breast Cancer Cells with Few Side Effects in Normal Cells

The neddylation inhibitor MLN4924 inhibits proliferation and triggers apoptosis of oral cancer cells but not for normal cells

Investigation of Hyperlipidemia Associated with Increased Levels of Oxidized Low-Density Lipoproteins and 8-Hydroxy-2´-Deoxyguanosine

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