8-Hydroxydeoxyguanosine in leukocyte DNA and urine of quartz-exposed workers and patients with silicosis

Pilger, Alexander; Germadnik, D.; Schäffer, Andreas; Theiler, Arnold; Pils, P; Sluka, F.; Winker, N.; Rüdiger, H. W.

doi:10.1007/s004200000117

Cited by 44 publications

(26 citation statements)

References 27 publications

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“…The lack of correlation between the 8-oxodG concentrations in lymphocytes and urine is in agreement with other studies (26). Correlations may not be expected as 8-oxodG concentration in nuclear cell DNA reflects the balance between formation and repair, whereas the urinary excretion supposedly reflects the summed rates of formation and turnover of 8-oxodG in nuclear and mitochondrial DNA as well as the nucleotide pool in the whole body (14).…”

Section: Discussionsupporting

confidence: 90%

Transition Metals in Personal Samples of PM2.5 and Oxidative Stress in Human Volunteers

Sørensen

Schins²,

Hertel³

et al. 2005

Cancer Epidemiology, Biomarkers &Amp; Prevention

136

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Ambient particulate matter (PM) has been associated with increased risk of lung cancer. One proposed mechanism is that PM induces oxidative stress mediated by transition metals contained within this mixture. We examined the relationship between the personal exposure to watersoluble transition metals in PM 2.5 and oxidative DNA damage. In 49 students from central Copenhagen, we determined PM 2.5 exposure by personal sampling twice in 1 year, and measured in these PM 2.5 samples the concentration of the soluble transition metals vanadium, chromium, iron, nickel, copper, and platinum. Collected lymphocytes and 24-hour urine samples were analyzed for DNA damage in terms of 7-hydro-8-oxo-2V-deoxyguanosine (8-oxodG). We found that the 8-oxodG concentration in lymphocytes was significantly associated with the vanadium and chromium concentrations with a 1.9% increase in 8-oxodG per 1 Mg/L increase in the vanadium concentration and a 2.2% increase in 8-oxodG per 1 Mg/L increase in the chromium concentration. We have previously reported that in this study population the personal exposure to PM 2.5 was associated with an increase in 8-oxodG in lymphocytes. However, vanadium and chromium were associated with the 8-oxodG concentration in lymphocytes independently of the PM 2.5 mass concentration. The four other transition metals were not associated with 8-oxodG in lymphocytes and none of the transition metals was significantly associated with 8-oxodG in urine. Our results could indicate that vanadium and chromium present in PM 2.5 have an effect on oxidative DNA damage that is independent of particle mass and/or other possible toxic compounds contained within this particulate mixture.

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Section: Discussionsupporting

confidence: 90%

Transition Metals in Personal Samples of PM2.5 and Oxidative Stress in Human Volunteers

Sørensen

Schins²,

Hertel³

et al. 2005

Cancer Epidemiology, Biomarkers &Amp; Prevention

136

View full text Add to dashboard Cite

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“…Overall, this has led to urinary measurements of thymine glycol being superseded by those of 8-oxodG and the subsequent widespread use of HPLC ECD. A number of methods based on column-switching HPLC with regular reverse phase (usually C18) separative columns and ECD for assay of 8-oxodG have been developed (24)(25)(26)(27)(28). The pre-purification, or extraction, columns have differed in their packing material, using generally C18 or ion exchange separation approaches.…”

Section: Methods Of Analysismentioning

confidence: 99%

Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine

Cooke

Oliński²,

Loft³

2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

206

147

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Background: Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety of diseases. The noninvasive assessment of such damage, i.e., in urine, and application to large-scale human studies are vital to understanding this role and devising intervention strategies. Methods: We have reviewed the literature to establish the status quo with regard to the methods and meaning of measuring DNA oxidation products in urine. Results: Most of the literature focus upon 8-oxo-7,8-dihydro-2 ¶-deoxyguanosine (8-oxodG), and whereas a large number of these reports concern clinical conditions, there remains (a) lack of consensus between methods, (b) possible contribution from diet and/or cell death, (c) no definitive DNA repair source of urinary 2 ¶-deoxyribonucleoside lesions, and (d) no reference ranges for healthy or diseased individuals. Conclusions: The origin of 8-oxodG is not identified; however, recent cell culture studies suggest that the

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“…In this study no association between 8-OHdG and the individual cumulative dust exposure was found, nor was there a synergistic eVect between smoking and occupational exposure. Pilger et al (2000) determined urinary 8-OHdG and 8-OHdG in leukocyte DNA of 63 workers occupationally exposed to quartz and 42 patients with silicosis. No signiWcant diVerences in 8-OHdG between healthy workers and silicosis patients were observed.…”

Section: Silicamentioning

confidence: 99%

8-Hydroxy-2′-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures

Pilger

Rüdiger

2006

Int Arch Occup Environ Health

Self Cite

300

204

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Oxidative DNA damage is considered to play an important role in pathophysiological processes, ageing and cancer. So far major interest has been on measuring 8-hydroxy-2'-deoxyguanosine (8-OHdG), the preferred methods relying on HPLC or GC-mass spectrometry. The high biological relevance of 8-OHdG is due to its ability to induce G-->T transversions, which are among the most frequent somatic mutations found in human cancers. Effects of workplace exposures on the level of white blood cell 8-OHdG or urinary 8-OHdG have been reported with controversial results. Exposures examined include asbestos, azo-dyes, benzene, fine particulate matter (PM(2.5)), glassworks, polycyclic aromatic hydrocarbons (PAHs), rubber manufacturing, silica, metals, styrene, toluene and xylenes. The available data indicate that there is still a lack of well established dose-response relations between occupational or environmental exposures and the induction of 8-OHdG. Smoking has been most consistently identified as a confounder for 8-OHdG, but various occupational studies did not reveal higher levels of 8-OHdG in smokers. Despite the conflicting results, the reported studies show promise for 8-OHdG as a biomarker of oxidative stress associated with chemical exposure. However, there are critical aspects related to the analytical challenge, artifactual production of 8-OHdG, inter- and intra-individual variation, confounding factors and inter-laboratory differences, implying that further work is needed to reach a consensus on the background level of 8-OHdG.

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8-Hydroxydeoxyguanosine in leukocyte DNA and urine of quartz-exposed workers and patients with silicosis

Abstract: Our findings suggest that a less effective repair of 8-OHdG is associated with a higher degree of pulmonary airway obstruction in patients with silicosis.

Cited by 44 publications

References 27 publications

Transition Metals in Personal Samples of PM2.5 and Oxidative Stress in Human Volunteers

Transition Metals in Personal Samples of PM2.5 and Oxidative Stress in Human Volunteers

Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine

8-Hydroxy-2′-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures

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