[80] Assessment of leukocyte involvement during ischemia and reperfusion of intestine

Grisham, Matthew B.; Benoit, Joseph N.; Granger, D. Neil

doi:10.1016/0076-6879(90)86172-r

Cited by 287 publications

(160 citation statements)

References 10 publications

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“…MPO activity was assessed as a marker of neutrophil infiltration according to the methods of Grisham et al [20] with slight modifications. In all animals one sample from the distal colon was obtained.…”

Section: Assessment Of Leukocyte Involvementmentioning

confidence: 99%

Protective effect of ellagic acid, a natural polyphenolic compound, in a murine model of Crohn's disease

Rosillo

Sánchéz-Hidalgo

Cárdeno

et al. 2011

Biochemical Pharmacology

153

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 of 37A c c e p t e d M a n u s c r i p t

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Section: Assessment Of Leukocyte Involvementmentioning

confidence: 99%

Protective effect of ellagic acid, a natural polyphenolic compound, in a murine model of Crohn's disease

Rosillo

Sánchéz-Hidalgo

Cárdeno

et al. 2011

Biochemical Pharmacology

153

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“…A surrogate marker of inflammation, MPO activity, was also assessed in an intact piece of colon tissue from noninflamed and inflamed mice using a colourimetric assay as described by Grisham et al [9]. Colon tissue from 12 and 25 week old FVB and mdr1a -/-was used.…”

Section: Myeloperoxidase Activitymentioning

confidence: 99%

“…Therefore, for sequential sampling, both mdr1a -/-and FVB control mice were randomly divided into eight sampling groups (9,12,16,22,25,27, 29 and 31 weeks of age). All mice were weighed twice a week and carefully monitored for disease symptoms (weight loss, soft faeces, faecal bleeding, posture, gait and inactivity).…”

Section: Experimental Designmentioning

confidence: 99%

Characterization of intestinal inflammation and identification of related gene expression changes in mdr1a−/− mice

et al. 2007

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Multidrug resistance targeted mutation (mdr1a -/-) mice spontaneously develop intestinal inflammation. The aim of this study was to further characterize the intestinal inflammation in mdr1a -/-mice. Intestinal samples were collected to measure inflammation and gene expression changes over time. The first signs of inflammation occurred around 16 weeks of age and most mdr1a -/-mice developed inflammation between 16 and 27 weeks of age. The total histological injury score was the highest in the colon. The inflammatory lesions were transmural and discontinuous, revealing similarities to human inflammatory bowel diseases (IBD). Genes involved in inflammatory response pathways were upregulated whereas genes involved in biotransformation and transport were down-regulated in colonic epithelial cell scrapings of inflamed mdra1 -/-mice at 25 weeks of age compared to non-inflamed FVB mice. These results show overlap to human IBD and strengthen the use of this in vivo model to study human IBD. The anti-inflammatory regenerating islet-derived genes were expressed at a lower level during inflammation initiation in non-inflamed colonic epithelial cell scrapings of mdr1a -/-mice at 12 weeks of age. This result suggests that an insufficiently suppressed immune response could be crucial to the initiation and development of intestinal inflammation in mdr1a -/-mice.

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“…Myeloperoxidase is stored in the primary granules of neutrophils and the enzyme activity is a common measure of neutrophil accumulation. 32 We have found a significant decrease in testis tissue MPO activity after montelukast administration. These data suggest that the anti-inflammatory effects of Montelukast contribute to its protective role in testicular torsion.…”

Section: Discussionmentioning

confidence: 70%

Montelukast protects against testes ischemia/reperfusion injury in rats

Öztürk

Gideroğlu

et al. 2013

CUAJ

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Introduction: In this study, we investigate the effect of montelukast on histologic damage induced by testicular torsion-detorsion in rats. Methods: Twenty-one male Sprague-Dawley rats were separated into 3 groups, each containing 7 rats. A sham operation was performed in group 1 (control). In group 2 (ischemia-reperfusion [I-R]/untreated), 1-hour detorsion of the testis was performed after 6 hours of unilateral testicular torsion. In group 3 (I-R/dextroamphetamine), after performing the same surgical procedures as in group 2, montelukast was given intraperitoneally. In all experimental rats, ipsilateral orchiectomies were performed for histological examination and tissue malondialdehyde (MDA), glutathione and myeloperoxidase assays. Results: Montelukast treatment significantly decreased the I-Rinduced elevation in testes tissue MDA and glutathione levels were found to be preserved. The level of myeloperoxidase (MPO) activity was significantly increased in the testes tissue of the I-R/untreated group. However, in I-R/montelukast treatment group significantly decreased testes tissue MPO level. Histopathologically, the in the group 2 rats, edema, congestion, hemorrhage between seminiferous tubules and necrosis of the germinal cells were predominant features in sections. However, most of the specimens in the montelukast treated group 3 showed grades-I and II injury. Additionally, the testicular injury score was lower in group 3 rats compared with group 2. Conclusion: The current findings demonstrate that the montelukast decreased the severity of testicular injury by reversing the oxidative effects of testes I-R. RésuméIntroduction : Dans cette étude, nous examinons l'effet du montélukast sur les lésions tissulaires provoquées par torsion-détorsion testiculaire chez le rat. Méthodologie : Vingt-et-un rats Sprague-Dawley mâles ont été répartis en trois groupes de 7 rats. Une opération fictive a été réalisée dans le groupe 1 (groupe témoin). Dans le groupe 2 (ischémie-reperfusion / non traité), une détorsion testiculaire d'une heure a été réalisée après 6 heures de torsion testiculaire unilatérale. Dans le groupe 3 (ischémie-reperfusion / dextroamphét-amine), on a administré du montélukast par voie intrapéritonéale après les mêmes interventions chirurgicales que pour le groupe 2. Chez tous les rats, une orchidectomie homolatérale a été effectuée en vue d'un examen histologique et de mesures du malonaldéhyde, du glutathion et de la myéloperoxidase dans les tissus. Résultats :Le traitement par montélukast a significativement réduit l'élévation dans les tissus testiculaires provoquée par l'ischémie-reperfusion, mais les taux de malonaldéhyde et de glutathion sont demeurés les mêmes. Le niveau d'activité de la myéloperoxidase (MPO) a significativement augmenté dans les tissus testiculaires du groupe ayant subi l'ischémie-reperfusion sans traitement subséquent. Cependant, dans le groupe ayant subi l'ischémie-reperfusion avec traitement par montélukast, le taux de MPO dans les tissus testiculaires avait significativ...

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[80] Assessment of leukocyte involvement during ischemia and reperfusion of intestine

Cited by 287 publications

References 10 publications

Protective effect of ellagic acid, a natural polyphenolic compound, in a murine model of Crohn's disease

Protective effect of ellagic acid, a natural polyphenolic compound, in a murine model of Crohn's disease

Characterization of intestinal inflammation and identification of related gene expression changes in mdr1a−/− mice

Montelukast protects against testes ischemia/reperfusion injury in rats

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