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Elmore, Eugene; Siddiqui, Shazia; Desai, Neeraj; Moyer, Mary Pat; Steele, Vernon E.; Redpath, J. L.

doi:10.1023/a:1024453300493

Cited by 6 publications

(2 citation statements)

References 7 publications

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“…That means the methanolic soluble fraction has more cytotoxic activity since the existence of the polar compounds. According to Elmore et al (2002), the highest concentration of a test agent in cytotoxicity evaluation should be less than 1.000 μg/mL or 1.000 μM. If none of the concentrations of test agents exhibited cytotoxic effect in excess of 50% of cell populations, the test agent is considered non-toxic against the tested cell line.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity and apoptosis induction of avocado Persea americana Mill. seed extract on MCF-7 cancer cell line

Widiyastuti¹,

Pratiwi

Riyanto

et al. 2018

Indones. J. Biotechnol.

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Avocado Persea Americana Mill. is a commercially important crop and studies have shown that the pulp may have benefits to cardiovascular health, dermatological health and possibly anti-cancer activity. Avocado seeds have several medicinal properties such as anti-hyperglycemic, antimicrobial, antioxidant and anti-inflammation. This study aim to evaluate the effect of avocado seed extract on viability and apoptosis of breast cancer cell line MCF-7. The anticancer effect was evaluated by cytotoxic test using MTT assay and the effect on apoptosis and cell cycle was examined by flow cytometry method. The cytotoxic test showed that chloroform extract had strong cytotoxic activity against MCF-7 cell lines with IC50 value of 94.87 µg/mL. Furthermore, the chloroform extract was partitioned with methanol and yield of soluble methanol fraction (FLM) and non soluble methanol fraction (FTLM). The cytotoxic activity of the methanol soluble fraction (FLM) and non soluble methanol fraction (FTLM) against MCF-7 cell lines was increased with IC50 of 34.52 and 66.03 µg/mL, respectively. Flow cytometry analysis using annexin-V and propidium iodide staining revealed that methanol soluble fraction could induce apoptosis and modulating the cell cycle arrest in MCF-7 cell. This research indicated that avocado seed has a potency to induce apoptosis and as anti-proliferative to MCF-7 cells lines.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic activity and apoptosis induction of avocado Persea americana Mill. seed extract on MCF-7 cancer cell line

Widiyastuti¹,

Pratiwi

Riyanto

et al. 2018

Indones. J. Biotechnol.

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“…To determine the selectivity of the zafirlukast derivatives synthesized, we tested the most active compounds 22b, 23a, and 23b against immortalized oral keratinocyte cell line OKF6, along with zafirlukast as a control. [40][41][42][43] A preliminary LIVE/DEAD assay was performed after incubating the cells with compounds 22b, 23a, and 23b for 24 h (Fig. 5).…”

Section: Effect Of the Zafirlukast Derivatives On The Cell Viability mentioning

confidence: 99%

Novel zafirlukast derivatives exhibit selective antibacterial activity againstPorphyromonas gingivalis

et al. 2019

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Physical properties and biological activity of poly(butyl acrylate–styrene) nanoparticle emulsions prepared with conventional and polymerizable surfactants

Garay-Jimenez

Gergeres

Young

et al. 2009

Nanomedicine: Nanotechnology, Biology and Medicine

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Recent efforts in our laboratory have explored the use of polyacrylate nanoparticles in aqueous media as stable emulsions for potential applications in treating drug-resistant bacterial infections. These emulsions are made by emulsion polymerization of acrylated antibiotic compounds in a mixture of butyl acrylate and styrene (7:3 w:w) using sodium dodecyl sulfate (SDS) as a surfactant. Prior work in our group established that the emulsions required purification to remove toxicity associated with extraneous surfactant present in the media. This paper summarizes our investigations of poly(butyl acrylate-styrene) emulsions made using anionic, cationic, zwitterionic, and non-charged (amphiphilic) surfactants, as well as attachable surfactant monomers (surfmers), comparing the cytotoxicity and microbiological activity levels of the emulsion both before and after purification. Conflict of Interest Statement:Edward Turos is co-inventor on a US patent application by the University of South Florida for the polyacrylate nanoparticle antibiotics, the subject of this publication. Dr. Turos is also co-founder, chief scientific advisor, and shareholder of Nanopharma Technologies, Inc., a University of South Florida spin-out company. Nanopharma Technologies, Inc., has licensed the nanoparticles technology from University of South Florida for potential commercial development.Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptNanomedicine. Author manuscript; available in PMC 2010 December 1. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author ManuscriptOur results show that the attachment of a polymerizable surfmer onto the matrix of the nanoparticle neither improves nor diminishes cytotoxic or antibacterial effects of the emulsion, regardless of whether the emulsions are purified or not, and that the optimal properties are associated with the use of the non-ionic surfactants versus those carrying anionic, cationic, or zwitterionic charge. Incorporation of an N-thiolated β-lactam antibacterial agent onto the nanoparticle matrix via covalent attachment endows the emulsion with antibiotic properties against pathogenic bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), without changing the physical properties of the nanoparticles or their emulsions.

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Untitled

Cited by 6 publications

References 7 publications

Cytotoxic activity and apoptosis induction of avocado Persea americana Mill. seed extract on MCF-7 cancer cell line

Cytotoxic activity and apoptosis induction of avocado Persea americana Mill. seed extract on MCF-7 cancer cell line

Novel zafirlukast derivatives exhibit selective antibacterial activity againstPorphyromonas gingivalis

Physical properties and biological activity of poly(butyl acrylate–styrene) nanoparticle emulsions prepared with conventional and polymerizable surfactants

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