87 Effects of Horse Bone Extracts on Induced Postmenopausal Osteoporosis in Rats

Park, Sun-Soon; Yoon, Weon‐Jong; Lee, Hye-Ja; Kang, Gyeong-Jin; Yang, Eun Jin; Kim, Sung-mi; Choo, Chang-Su; Kang, Hee‐Kyoung; Yoo, Eun-Sook

doi:10.1016/j.cyto.2008.07.128

Cited by 2 publications

(3 citation statements)

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“…말은 우리나라에서 일반적으로 승용(乘用), 태용(駄用), 만 용(輓用), 식용(食用) 등의 목적으로 사용되어 왔으며 일부 유 럽국가와 일본에서 식용으로 소비되고 있다 [22]. 민간요법에 의하면 말은 주로 기억력 감퇴, 건망증, 치매, 신경통, 관절염, 골다공증에 좋다고 알려져 있어서 말고기, 말뼈 분말, 엑기스, 환등의 제품으로 제조되어 시판되고 있으나 이에 대한 연구는 주로 제주마육 사양 및 생산 [18], 말고기의 영양 및 식육가치 [16,28], 유전특성 [15], 번식에 관한 연구 [6]가 주로 진행되어 왔다.…”

Section: 서 론unclassified

“…그러나 최근 말의 뼈를 이용한 연구는 그리 많지 않으나 현재까지 보고된 말뼈의 효 능은 36개월령의 숫말의 넙적다리 뼈를 분쇄하여 50%의 아세 톤을 이용하여 추출한 시료의 급여가 성장기 흰쥐의 성장판의 길이와 장골길이를 유의적으로 증가시키고 말뼈의 무기물 함 량이 칼슘과 인의 비율이 2:1로서 골대사에 바람직한 영향을 주는 이상적인 비율을 갖고 있음이 보고되었다 [19]. 또한 말뼈 추출물을 폐경기 골다공증 흰쥐에 급여 후 골다공증 개선이 되었음을 보고되었다 [22] …”

Section: 서 론unclassified

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Antioxidation Effect of Leg Bone Extracts and Enzyme Hydrolysates from Jeju Crossbred Horses (Jeju Native Horse×Thoroughbred)

Kim¹,

Pak²,

Chae³

et al. 2013

Journal of Life Science

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This study was conducted to evaluate the antioxidation activity of Jeju crossbred horse (Jeju native horse × thoroughbred) leg bone extracts (HLBE) and its enzyme hydrolysates by determination of 1,1-diphenyl-2picryl-hydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzo thiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, ferric reducing/antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). HLBE was extracted with hot water for 24 hr and lyophilized. The lyophilized HLBE was hydrolyzed using multifect PR6L (MP), pepsin (PS), and a pepsin and pancreatin mixture (PSPC) for 4 hr at 60, 50, and 50°C, respectively. The hydrolysates were separated by a molecular weight of 3 kDa more or less. When the yield of HLBE was 100%, the yield of hydrolysates less than 3 kDa of MP, PS, and PSPC was 10.86, 3.26, and 8.00%, respectively. Enzyme hydrolysates with low molecular weight less than 3 kDa in MP and PSPC showed significantly higher DPPH, ABTS radical scavenging activity, and ORAC compared to HLBE and its hydrolysates with more than 3 kDa. However, the FRAP of the hydrolysates less than 3 kDa in PS was significantly higher than in MP. These results suggest that low molecular weight enzyme hydrolysates less than 3 kDa have more powerful antioxidation activity, especially when they are hydrolyzed by MP and PSPC rather than PS. Therefore, low molecular weight enzyme hydrolysates of HLBE hydrolyzed with MP and PSPC have significant potential as antioxidants in the food industry.Further in vivo studies are required to support the antioxidation activities of the hydrolysates in vitro.

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Section: 서 론unclassified

Antioxidation Effect of Leg Bone Extracts and Enzyme Hydrolysates from Jeju Crossbred Horses (Jeju Native Horse×Thoroughbred)

Kim¹,

Pak²,

Chae³

et al. 2013

Journal of Life Science

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“…One of the health benefits of these equine bone extracts is that they cause an increase in bone density and improve bone health. A study found that dietary supplementation with horse leg bone water extracts for 8 weeks significantly increases the uterine/body weight ratio and bone mineral density of female rats with postmenopausal osteoporosis ( Park et al ., 2010 ). In our previous study, a large antioxidant effect was observed in the low molecular weight peptide fraction of less than 3 kDa isolated from leg bone extracts via enzyme hydrolysis ( Kim et al ., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates

Kim

Chae

et al. 2014

Korean Journal for Food Science of Animal Resources

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This study focused on the anti-oxidative and collagenase- and elastase inhibition effects of low molecular weight peptides (LMP) from commercial Jeju horse leg bone hydrolysates (JHLB) on pancreatin, via enzymatic hydrolysis. Cell viability of dermal fibroblasts exposed to UVB radiation upon treatment with LMP from JHLB was evaluated. Determination of the antioxidant activity of various concentrations of LMP from JHLB were carried out by assessing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethybenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). The DPPH radical scavenging activity of LMP from JHLB (20 mg/mL) was 92.21% and ABTS radical scavenging activity (15 mg/mL) was 99.50%. FRAP activity (30 mg/mL) was 364.72 μM/TE and ORAC activity (1 mg/mL) was 101.85 μM/TE. The anti-wrinkle potential was assessed by evaluating the elastase- and collagenase inhibition potential of these LMP. We found that 200 mg/mL of LMP from JHLB inhibited elastase activity by 41.32%, and 100 mg/mL of LMP from JHLB inhibited collagenase activity by 91.32%. The cell viability of untreated HS68 human dermal fibroblasts was 45% when exposed to a UVB radiation dose of 100 mJ/cm2. After 24 h of incubation with 500 μg/mL LMP from JHLB, the cell viability increased to 60%. These results indicate that LMP from JHLB has potential utility as an anti-oxidant and anti-wrinkle agent in the food and cosmetic industry. Additional in vivo tests should be carried out to further characterize these potential benefits.

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87 Effects of Horse Bone Extracts on Induced Postmenopausal Osteoporosis in Rats

Cited by 2 publications

References 0 publications

Antioxidation Effect of Leg Bone Extracts and Enzyme Hydrolysates from Jeju Crossbred Horses (Jeju Native Horse×Thoroughbred)

Antioxidation Effect of Leg Bone Extracts and Enzyme Hydrolysates from Jeju Crossbred Horses (Jeju Native Horse×Thoroughbred)

Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates

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