9-Hydroxypheophorbide α-induced apoptotic death of MCF-7 breast cancer cells is mediated by c-Jun N-terminal kinase activation

Choi, Sung‐E; Sohn, Seonghyang; Cho, Jeong-woo; Shin, Eun-A; Song, Pill‐Soon; Kang, Youn-Bae

doi:10.1016/j.jphotobiol.2003.11.002

Cited by 23 publications

(19 citation statements)

References 20 publications

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“…As shown in Figure 5B, a leftward shift of the fluorescence curve, an As a new photosensitizer, it has been reported that 9-HPbDmediated PDT can inhibit the growth of the MCF-7 human breast cancer cell line and has an apoptosis-inducing effect. 13 In the current study, the photocytotoxicity of 9-HPbD was tested on AMC-HN-3 human head and neck carcinoma cells, which AMC-HN-3 cells treated with either 9-HPbD alone or light in the absence of 9-HPbD (Fig. 2), suggesting that 9-HPbD shared the same characteristic with other photosensitizers, which could minimize the undesirable effects of an antitumor drug towards the normal tissue during treatment.…”

Section: Discussionmentioning

confidence: 87%

“…9,10 A pheophorbide-derived photosensitizer was also developed for PDT to overcome skin photosensitivity. 11,12 In the current study, a novel pheophorbide-based photosensitizer, 9-hydroxypheophorbide α (9-HPbD), 13 was used to assess the photocytoxicity to AMC-HN-3 cancer cells. The mitochondrial events and endoplasmic reticulum (ER) stress induced by 9-HPbD-PDT, which ultimately led to apoptosis, were also investigated herein.…”

Section: Introductionmentioning

confidence: 99%

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Photodynamic therapy with 9-hydroxypheophorbide α on AMC-HN-3 human head and neck cancer cells: Induction of apoptosis via photoactivation of mitochondria and endoplasmic reticulum

Chung

He²,

Shin³

et al. 2009

Cancer Biology & Therapy

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Apoptotic mechanism induced by 9-HPbD-PDT in AMC-HN-3 cellsSkin phototoxicity is one of the main side effects of photodynamic therapy (PDT). To overcome this problem, some new photosensitizers have been developed with longer absorbance wavelengths and shorter half-life in the body. In this study, we investigated the mechanism of PDT mediated by a new chlorophyll derivative photosensitizer, 9-hydroxypheophorbide α (9-HPbD), on AMC-HN-3 cancer cells. Phototoxicity and apoptosis on AMC-HN-3 cells induced by 9-HPbD was exhibited in a time-and dose-dependent manner. Mitochondria and endoplasmic reticulum (ER) were observed as preferential sites of 9-HPbD accumulation. Photoactivation of 9-HPbD-loaded AMC-HN-3 cells led to a rapid generation of reactive oxygen species (ROS) at 30 min, followed by a loss of mitochondrial membrane potential (MMP) at 2 h, translocation of apoptosisinducing factor (AIF) at 2 h, and the release of cytochrome c at 3 h following PDT. Caspase-12, an important caspase involved in ER-induced apoptosis, and C/EBP homologous protein (CHOP), an ER stress inducible transcription factor, were also upregulated after PDT (3-12 h and 6-12 h, respectively). Subsequently, activation of caspase-9 at 6 h, caspase-3 and PARP at 12 h also occurred in PDT-treated AMC-HN-3 cells.The above observations demonstrate that both mitochondria and ER serve not only as the sites of sensitizer binding, but also the subcellular targets of 9-HPbD-PDT, effective activation of which is responsible for 9-HPbD PDT-induced apoptosis in AMC-HN-3 cells.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Photodynamic therapy with 9-hydroxypheophorbide α on AMC-HN-3 human head and neck cancer cells: Induction of apoptosis via photoactivation of mitochondria and endoplasmic reticulum

Chung

He²,

Shin³

et al. 2009

Cancer Biology & Therapy

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“…Up-regulation of caspase-3 and -9 was also observed after a combined modality with 9-HPbD-PDT and carboplatin in AMC-HN-3 cells (13). However, activation of caspase-9 was not detected in 9-HPbD-induced MCF-7 cell death (12). A probable different localization of 9-HPbD in MCF-7 and AMC-HN-3 cells may lead to the distinct activation of caspase-9.…”

Section: Discussionmentioning

confidence: 79%

“…As a new photosensitizer, 9-HPbD-PDT was found to inhibit the growth of the MCF-7 human breast cancer cell line and had an apoptosis-inducing effect (12). In the present study, the photocytotoxicity of 9-HPbD was tested in a doseand time-dependent response in AMC-HN-3 human laryngeal cancer cells.…”

Section: Discussionmentioning

confidence: 89%

“…With a relatively longer absorbance wavelength and shorter half-life in the body, a novel pheophorbide-based photosensitizer, 9-hydroxypheophorbide-· (9-HPbD), displayed an apoptosis-inducing effect and growth suppression in the MFC-7 human breast cancer cell line (12). We recently showed that 9-HPbD-PDT used in combination with carboplatin enhanced cytotoxic and apoptotic effects in AMC-HN-3 laryngeal cancer cells (13).…”

Section: Introductionmentioning

confidence: 99%

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Photoactivation of 9-hydroxypheophorbide α triggers apoptosis through the reactive oxygen species-mediated mitochondrial pathway and endoplasmic reticulum stress in AMC-HN-3 laryngeal cancer cells

Ahn²,

Shin³

et al. 2010

Int J Oncol

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Abstract. Photodynamic therapy (PDT) is a promising treatment modality for a variety of cancers. It utilizes lightabsorbing compounds combined with direct illumination to generate reactive oxygen species in photosensitizer-targeted tumor cells resulting in the final photodamage of tumors. Recently, we demonstrated that a combination modality of 9-hydroxypheophorbide · (9-HPbD)-based PDT and carboplatin exerts enhanced cytotoxic and apoptotic effects on AMC-HN-3 laryngeal cancer cells. The present study aimed to investigate the potential apoptotic pathways initiated by 9-HPbD-PDT-mediated reactive oxygen species (ROS) in AMC-HN-3 cells. Cytotoxicity and apoptosis induced by 9-HPbD-PDT were exhibited in a ROS-dependent manner. Mitochondria and the endoplasmic reticulum (ER) were observed as preferential sites of 9-HPbD accumulation in AMC-HN-3 cells. ROS induced by 9-HPbD-PDT directly led to downregulated expression of Bcl-2, loss of mitochondrial membrane potential, release of cytochrome c from mitochondria, elevation of intracellular calcium due to ER stress, as well as induction of CHOP and activation of caspase-3, -8, -9 and -12. Our results demonstrated that ROS induced by 9-HPbD-PDT play a causative role in triggering mitochondrial events, ER stress and probable involvement of the extrinsic apoptotic pathway in AMC-HN-3 cells.

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Role in Therapeutic Sciences

Kannaujiya

Sundaram

Sinha

2017

Phycobiliproteins: Recent Developments and Future Applications

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9-Hydroxypheophorbide α-induced apoptotic death of MCF-7 breast cancer cells is mediated by c-Jun N-terminal kinase activation

Cited by 23 publications

References 20 publications

Photodynamic therapy with 9-hydroxypheophorbide α on AMC-HN-3 human head and neck cancer cells: Induction of apoptosis via photoactivation of mitochondria and endoplasmic reticulum

Photodynamic therapy with 9-hydroxypheophorbide α on AMC-HN-3 human head and neck cancer cells: Induction of apoptosis via photoactivation of mitochondria and endoplasmic reticulum

Photoactivation of 9-hydroxypheophorbide α triggers apoptosis through the reactive oxygen species-mediated mitochondrial pathway and endoplasmic reticulum stress in AMC-HN-3 laryngeal cancer cells

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