(−)-9′-O-(α-l-Rhamnopyranosyl)lyoniresinol from Lespedeza cuneata suppresses ovarian cancer cell proliferation through induction of apoptosis

Aviculin Isolated from Lespedeza cuneata Induce Apoptosis in Breast Cancer Cells through Mitochondria-Mediated Caspase Activation Pathway

“…Metabolic activity was reduced to 83.08% ± 1.82% after treatment with 100 μM (+)-5′methoxyisolariciresinol-9′-O-α-L-rhamnoside (4) ( Figure 3D). Likewise, kaempferol-7-O-β-Dglucopyranoside (5) and astragaline (6) showed metabolic activity decreased to 94.96% ± 0.43% and 89.05% ± 2.03%, respectively, after treatment with 100 μM ( Figures 3E,F). Orientin 7showed suppressed metabolic activity to 52.94% ± 0.62% after treatment with 100 μM.…”

“…Previous pharmacological studies showed that extracts of Lespedeza cuneata can be used to treat hematuria and insomnia. In addition, extracts of L. cuneata were reported to have hepatoprotective and antidiabetic effects [4], anticancer and antioxidative activity, and inhibitory effects on cell proliferation [5]. The useful cosmetic effects of L. cuneata extracts, such as antiwrinkle, antimelanogenic, anti-aging, and anti-oxidative effects, have also been reported [6,7].…”

“…As part of the continuing research into bioactive compounds from Korean medicinal plant sources [13][14][15][16][17], the MeOH extract of the aerial portion of L. cuneata was found to exhibit cytotoxic effects on human ovarian carcinoma cells [5]. In our recent study, bioassay-guided fractionation of the MeOH extract using repeated chromatography resulted in the isolation of (−)-9 -O-(α-l-rhamnopyranosyl)lyoniresinol, which inhibited the proliferation of A2780 human ovarian carcinoma cells through the induction of apoptosis [5]. In our ongoing study of L. cuneata, the phytochemical analysis of the L. cuneata MeOH extract led to the isolation of two new lignan glycosides along with three known lignan glycosides and nine known flavonoid glycosides, some of which exhibited weak cytotoxicity against four human breast cancer cell lines (Bt549, MCF-7, MDA-MB-231, and HCC70) [18].…”

“…In the current study, we found in a screening test that the MeOH extract of L. cuneata exerted inhibitory effect on metabolic activity in MCF-7 human breast cancer cells, which led us to perform further phytochemical analysis and identify the bioactive compound(s) responsible for these effects. Bioassay-guided fractionation and chemical investigation of the MeOH extract led to the isolation of nine compounds (four lignan glycosides (1)(2)(3)(4), three flavonoid glycosides (5)(6)(7), and two phenolic compounds (8 and 9)). The structures of the isolated compounds were identified by LC-MS analysis and their spectroscopic data and physical data were compared with previously reported values.…”

Lignan Glycosides and Flavonoid Glycosides from the Aerial Portion of Lespedeza cuneata and Their Biological Evaluations

“…As part of a continuing search for bioactive constituents from Korean medicinal plant sources [ 12 , 13 , 14 ], the methanol (MeOH) extract of the aerial portion of L. cuneata was found to exhibit cytotoxic effects on human ovarian carcinoma cells [ 15 ]. In our recent study, bioassay-guided fractionation and repeated chromatography of the MeOH extract of L. cuneata resulted in isolation of (−)-9′- O -(α- l -rhamnopyranosyl)lyoniresinol, which suppresses the proliferation of A2780 human ovarian carcinoma cells through induction of apoptosis [ 15 ]. In the current study investigating bioactive compounds from the aerial portion of L. cuneata , further phytochemical analysis was carried out, which led to the isolation of two new lignan glycosides ( 1 , 2 ) along with three known lignan glycosides ( 3 – 7 ) and nine known flavonoid glycosides ( 8 – 14 ).…”